[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-369661-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-369661-en":131},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","bitter-taste-receptor-agonists-induce-apoptosis-in-papillary-thyroid-cancer","Bitter Taste Receptor Agonists Induce Apoptosis in Papillary Thyroid Cancer","","Papillary thyroid carcinoma is a common thyroid malignancy with substantial recurrence. Bitter taste receptors (T2Rs) and their genes (TAS2Rs) have been implicated in survival regulation in solid tumors, motivating investigation of their roles in papillary thyroid cancer cells. The study analyzed T2R expression and function across three PTC cell lines, measuring calcium responses to bitter agonists and assessing viability and apoptosis.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/bitter-taste-receptor-agonists-induce-apoptosis-in-papillary-thyroid-cancer/369661/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/bitter-taste-receptor-agonists-induce-apoptosis-in-papillary-thyroid-cancer/369661.png","ImageObject",300,407,{"name":42,"@type":43},"Đào","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-30","2026-09-23",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",12,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"Which bitter taste receptor gene showed consistent expression across the PTC cell lines?","Question",{"text":63,"@type":64},"TAS2R14 was consistently highly expressed in all three analyzed papillary thyroid cancer cell lines.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How did the bitter agonists affect calcium signaling in PTC cells?",{"text":68,"@type":64},"Five bitter agonists produced significant cytoplasmic and mitochondrial calcium responses across all cell lines.",{"name":70,"@type":61,"acceptedAnswer":71},"What was the relationship between TAS2R expression and patient outcomes?",{"text":72,"@type":64},"Higher TAS2R14 expression correlated with better progression-free survival in patients (p \u003C 0.05).","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},369661,1790330807,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,110,115,120,123,127],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":108,"slug":109},7,40,"healthcare",{"id":111,"doc_module":4,"doc_module_name":25,"category_name":112,"show_sort_weight":113,"slug":114},8,"Research & Report",30,"research-report",{"id":116,"doc_module":4,"doc_module_name":25,"category_name":117,"show_sort_weight":118,"slug":119},9,"Religion & Spirituality",20,"religion-spirituality",{"id":118,"doc_module":4,"doc_module_name":25,"category_name":121,"show_sort_weight":118,"slug":122},"World Cup","world-cup",{"id":124,"doc_module":4,"doc_module_name":25,"category_name":125,"show_sort_weight":124,"slug":126},10,"Lifestyle","lifestyle",{"id":128,"doc_module":4,"doc_module_name":25,"category_name":129,"show_sort_weight":97,"slug":130},19,"General","general",{"code":4,"msg":82,"data":132},{"doc_id":79,"user_id":133,"nickname":42,"user_avatar":134,"doc_module":4,"category_id":107,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":140,"language":141,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":12,"update_tm":145,"read_time":146},1374402968488,"https://ap-avatar.wpscdn.com/davatar_29158cc5080c5b710cf443261637dec0","Head & Neck  \nORIGINAL ARTICLE  OPEN ACCESS   \nBitter Taste Receptor Agonists Induce Apoptosis in Papillary Thyroid Cancer  \nKimberly Wei1  | Brianna L. Hill1 | Joel C. Thompson1 | Zoey A. Miller1 | Arielle Mueller1 | Robert J. Lee1,2 | Ryan M. Carey1   \n1Department of Otorhinolaryngology – Head and Neck Surgery, University of Pennsylvania, Perelman School of Medicine, Philadelphia, Pennsylvania,  \nUSA | 2Department of Physiology, Perelman School of Medicine, Philadelphia, Pennsylvania, USA Correspondence: Ryan M. Carey ([ryan.carey@pennmedicine.upenn.edu](ryan.carey@pennmedicine.upenn.edu))  \nReceived: 15 October 2024 | Revised: 10 February 2025 | Accepted: 11 February 2025  \nFunding: This work was supported by the American Head and Neck Society Pilot Grant (to R.M.C.), a McCabe Foundation Fellowship Grant (to R.M.C.), R01AI167971 (to R.J.L.), and R21DC020041 (to R.J.L.), the National Institutes of Health, National Institute of Allergy and Infectious Diseases.  \nKeywords: apoptosis | calcium | cell signaling | papillary thyroid cancer | taste receptors  \nABSTRACT  \nBackground: Papillary thyroid carcinoma (PTC) is the most common thyroid malignancy, with a 20% recurrence rate. Bitter taste receptors (T2Rs) and their genes (TAS2Rs) may regulate survival in solid tumors. This study examined T2R expression and function in PTC cells.  \nMethods: Three PTC cell lines (MDA-T32, MDA-T68, and MDA-T85) were analyzed for expression using RT-qPCR and immunofluorescence. Live cell imaging measured calcium responses to six bitter agonists. Viability and apoptosis effects were assessed using crystal violet and caspase 3/7 activation assays. Genome analysis of survival was conducted.  \nResults: TAS2R14 was consistently highly expressed in all cell lines. Five bitter agonists produced significant cytoplasmic and mitochondrial calcium responses across all cell lines. All bitter agonists significantly decreased viability and induced apoptosis. Higher TAS2R14 expression correlated with better progression-free survival in patients (p \u003C 0.05) .  \nConclusions: T2R activation by bitter agonists induces apoptosis, and higher TAS2R expression is associated with survival, suggesting potential therapeutic relevance in thyroid cancer management.  \n1 | Introduction  \nPapillary thyroid cancer (PTC) is a differentiated epithelial tumor that arises from the follicular cells of the thyroid. It is the most common thyroid neoplasm, constituting about 80% of all cases, and its incidence and prevalence are on the rise. Risk factors include female gender (3:1 female-to-male ratio), previous exposure to ionizing radiation, and rare hereditary conditions such as Cowden syndrome [1] . In general, PTC has the best overall prognosis out of all thyroid carcinomas, with a 93%  \n10-year survival rate [2] . However, recurrence rates range from 15% to 30% due to PTC's propensity for metastasis through the lymphatics. Patients with identified lymph node (LN) metastases have worse relapse-free and overall survival (OS) [3–5] . In one large-scale study, the recurrence of PTC presenting with LN metastases was reported to be 31.5%, and out of the patients with recurrent PTC, 40% died of thyroid cancer [6] .  \nThe current standard of care is based on the extent of disease and includes surgery (thyroid lobectomy or total thyroidectomy)  \n\n| This study was presented and awarded Best Thyroid Poster at the AHNS 2024 Annual Meeting at COSM. |\n| --- |\n| This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.\u003Cbr>© 2025 The Author(s). Head & Neck published by Wiley Periodicals LLC. |\n\nHead & Neck, 2025; 47:2101–2113 2101  \n[https://doi.org/10.1002/hed.28120](https://doi.org/10.1002/hed.28120)  \npotentially followed by radioactive iodine (RAI) ablation and life-long ","cbCaipBg5uO5NsKO","https://ap.wps.com/l/cbCaipBg5uO5NsKO","pdf",7433251,13,"English","# Abstract\n# Background\n# Methods\n## Cell line analysis and gene expression\n## Calcium imaging and apoptosis assays\n# Results\n# Conclusions\n# Introduction\n## Disease context and clinical management\n## Role of T2Rs in cancers","[{\"question\":\"Which bitter taste receptor gene showed consistent expression across the PTC cell lines?\",\"answer\":\"TAS2R14 was consistently highly expressed in all three analyzed papillary thyroid cancer cell lines.\"},{\"question\":\"How did the bitter agonists affect calcium signaling in PTC cells?\",\"answer\":\"Five bitter agonists produced significant cytoplasmic and mitochondrial calcium responses across all cell lines.\"},{\"question\":\"What was the relationship between TAS2R expression and patient outcomes?\",\"answer\":\"Higher TAS2R14 expression correlated with better progression-free survival in patients (p \\u003c 0.05).\"}]","Bitter Taste Receptor Agonists Induce Apoptosis in Papillary Thyroid Cancer | PDF",1790183963,33]