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Single nucleotide polymorphisms and altered ERCC activity are discussed as contributors to impaired DNA repair, genomic instability, and increased cancer risk. Using publicly available data spanning 33 cancer types, ERCC expression associations are evaluated for patient prognosis and clinical features. ERCC2 is additionally tested in bladder cancer with in vitro functional assays, while multiple testing correction (Benjamini-Hochberg) supports the reliability of identified correlations with prognosis, tumor microenvironment, and immune-related indicators.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/bioinformatics-analysis-of-ercc-family-in-pan-cancer-and-ercc2-in-bladder-cancer/332392/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/bioinformatics-analysis-of-ercc-family-in-pan-cancer-and-ercc2-in-bladder-cancer/332392.png","ImageObject",300,407,{"name":92,"@type":93},"Ezra","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-13","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":52},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What biological problem does the study address?","Question",{"text":112,"@type":113},"The study addresses how DNA repair gene alterations, especially in the ERCC family, may impair nucleotide excision repair, contributing to genetic instability and higher cancer risk.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were ERCC-related associations investigated across cancers?",{"text":117,"@type":113},"Public datasets from 33 cancer types were used to analyze relationships between ERCC gene expression, patient prognosis, and clinical features, with Benjamini-Hochberg correction for multiple testing.",{"name":119,"@type":110,"acceptedAnswer":120},"What experiments were performed to validate ERCC2 in bladder cancer?",{"text":121,"@type":113},"ERCC2’s role in bladder cancer was validated using in vitro assays including CCK-8, colony formation, wound healing, and Transwell assays.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},332392,1790541573,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":52,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":46,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":31,"preview_page_count":4},1099514068035,"https://ap-avatar.wpscdn.com/davatar_276721f389ce27ea32af1340a28f341c","TYPE Original Research PUBLISHED 13 August 2024  \nDOI 10.3389/fimmu.2024.1402548  \nOPEN ACCESS  \nEDITED BY  \nYonghao Zhan,  \nZhengzhou University, China  \nREVIEWED BY Jiwei Huang,  \nShanghai Jiao Tong University, China Chunyang Wang,  \nHarbin Medical University, China  \n*CORRESPONDENCE Hailong Hu  \n [huhailong@tmu.edu.cn](huhailong@tmu.edu.cn)[ ](huhailong@tmu.edu.cn)Jiangping Wang  \n [mingzhujp@126.com](mingzhujp@126.com)  \n†These authors have contributed equally to this work and share first authorship  \nRECEIVED 17 March 2024  \nACCEPTED 25 July 2024  \nPUBLISHED 13 August 2024  \nCITATION  \nZhang S, Guan Z, Xia Q, Shen C, Hu H and Wang J (2024) Bioinformatics analysis of ERCC family in pan-cancer and ERCC2 in bladder cancer.  \nFront. Immunol. 15:1402548 .  \ndoi: 10.3389/fimmu.2024.1402548  \nCOPYRIGHT  \n© 2024 Zhang, Guan, Xia, Shen, Hu and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.  \nBioinformatics analysis of ERCC family in pan-cancer and ERCC2 in bladder cancer  \nSiyang Zhang 1†, Zhenghui Guan 1,2†, Qiangqiang Xia 3†, Chong Shen 4,5, Hailong Hu 4,5* and Jiangping Wang 1*  \n1 Department of Urology, The affiliated Taizhou People’s Hospital of Nanjing Medical University, Taizhou School of Clinical Medicine, Nanjing Medical University, Taizhou, Jiangsu, China,  \n2 Postgraduate Training Base of Dalian Medical University, The affiliated Taizhou People’s Hospital of Nanjing Medical University, Taizhou School of Clinical Medicine, Nanjing Medical University, Taizhou, Jiangsu, China, 3 Emergency Surgery, Yongcheng People’s Hospital of Henan Province, Henan, Shangqiu, China, 4 Department of Urology, Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, Tianjin, China, 5Tianjin Key Laboratory of Urology, Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, Tianjin, China  \nBackground: Single nucleotide polymorphisms (SNPs) in DNA repair genes can impair protein function and hinder DNA repair, leading to genetic instability and increased cancer risk. The Excision Repair Cross-Complementation (ERCC) family plays a crucial role in nucleotide excision repair, yet their comprehensive multi-omics characterization and roles in tumor prognosis and immune microenvironment remain unexplored.  \nMethods and materials: We performed bioinformatics analysis using publicly available data from 33 cancer types to investigate associations between ERCC gene expression, patient prognosis, and clinical features. We also validated the role of ERCC2 in bladder cancer through in vitro assays, including CCK-8, colony formation, wound healing, and Transwell assays.  \nResults: By utilizing the most recent database, we have conducted an analysis that reveals associations between variations in ERCC expression across multiple cancer types and both patient prognosis and the tumor microenvironment. To ensure the reliability of our findings, we applied the Benjamini-Hochberg procedure to adjust for multiple testing. After correction, we identified that ERCC expression levels remained significantly correlated with patient prognosis in various cancer types (p \u003C 0 . 05) . In addition, according to the results of drug sensitivity studies of anticancer drugs, there is a large correlation between ERCC expression and the sensitivity of different anticancer drugs. Finally, in vitro cell behavioral assays determined that knockdown of ERCC2 gene expression significantly inhibited the proliferation, migration and invasion of bladder cancer cells.  \nConclusion: Through in-depth exploration of ERCC differential expression and its","cbCait1syjaps9Tz","https://ap.wps.com/l/cbCait1syjaps9Tz","pdf",32010924,"English","# Background\n# Methods and materials\n# Results\n# Conclusion\n# Key findings and implications","[{\"question\":\"What biological problem does the study address?\",\"answer\":\"The study addresses how DNA repair gene alterations, especially in the ERCC family, may impair nucleotide excision repair, contributing to genetic instability and higher cancer risk.\"},{\"question\":\"How were ERCC-related associations investigated across cancers?\",\"answer\":\"Public datasets from 33 cancer types were used to analyze relationships between ERCC gene expression, patient prognosis, and clinical features, with Benjamini-Hochberg correction for multiple testing.\"},{\"question\":\"What experiments were performed to validate ERCC2 in bladder cancer?\",\"answer\":\"ERCC2’s role in bladder cancer was validated using in vitro assays including CCK-8, colony formation, wound healing, and Transwell assays.\"}]","Bioinformatics analysis of ERCC family in pan-cancer and ERCC2 in bladder cancer | PDF",1789998042]