[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-328226-105":59,"doc-detail-328226-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","bioinformatic-identification-of-tp53-gene-mutation-hotspots-in-colorectal-cancer","Bioinformatic Identification of TP53 Gene Mutation Hotspots in Colorectal Cancer","","Mutations and inactivation of the TP53 gene are commonly seen across malignancies, and identifying mutation hotspots is important because hotspots suggest a higher likelihood of cancer development. This study applies bioinformatic detection of TP53 mutation hotspots in malignant colon neoplasms using self-developed software (version 1). It compares TP53 sequences from 50 healthy individuals and 50 colorectal carcinoma patients, focusing on altered exons and mutation distribution.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/bioinformatic-identification-of-tp53-gene-mutation-hotspots-in-colorectal-cancer/328226/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/bioinformatic-identification-of-tp53-gene-mutation-hotspots-in-colorectal-cancer/328226.png","ImageObject",300,407,{"name":92,"@type":93},"Maeve","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-02","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the goal of the bioinformatic study on TP53 mutation hotspots?","Question",{"text":112,"@type":113},"To detect TP53 gene mutation hotspots in patients diagnosed with malignant colon neoplasms using self-developed software (version 1).","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were healthy and colorectal cancer samples compared in the study?",{"text":117,"@type":113},"TP53 gene sequences from 50 healthy individuals were compared with sequences from 50 patients with colorectal carcinoma.",{"name":119,"@type":110,"acceptedAnswer":120},"Which TP53 exons showed the most frequent mutations in colorectal cancer samples?",{"text":121,"@type":113},"The most frequent mutations were observed in exons 5 and 8, with 12 mutations per exon, among the cancer patient samples.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},328226,1789996555,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":41,"preview_page_count":4},5909877438554,"https://ap-avatar.wpscdn.com/avatar/5600025385ad2bf12a7?_k=1778553567797529272","Article  \nBioinformatic Identification of TP53 Gene Mutation Hotspots in Colorectal Cancer  \nZsolt Kovács 1,2, Haruhiko Sugimura 3, Tamás Attila György 1,2, Eva Osvath 1,2,4, Felix Manirakiza 5 and Simona Gurzu 1,2,6, *  \nCitation: Kovács, Z.; Sugimura, H.; György, T.A.; Osvath, E.; Manirakiza, F.; Gurzu, S. Bioinformatic Identification of TP53 Gene Mutation Hotspots in Colorectal Cancer. Int. J. Mol. Sci. 2024, 25, 6612. [https://](https://)[ ](https://)[doi.org/10.3390/ijms25126612](doi.org/10.3390/ijms25126612)  \nAcademic Editor: Tianhua Niu  \nReceived: 14 April 2024  \nRevised: 12 June 2024  \nAccepted: 13 June 2024  \nPublished: 15 June 2024  \nCopyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://](https://)[ ](https://)[creativecommons.org/licenses/by/](creativecommons.org/licenses/by/)[ ](creativecommons.org/licenses/by/)[4.0/](4.0/)) .  \n1 Department of Pathology,“George Emil Palade” University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540139 Targu Mures, Romania; [zsolt.kovacs@umfst.ro](zsolt.kovacs@umfst.ro) (Z.K.);  \n[gyorgytomims@gmail.com](gyorgytomims@gmail.com) (T.A.G.); [osvath_evi@yahoo.com](osvath_evi@yahoo.com) (E.O.)  \n2 Research Center of Oncopathology and Translational Research (CCOMT), 540139 Targu Mures, Romania  \n3 Sasaki Institute Sasaki Foundation, Tokyo 101-0062, Japan; [hsugimur@po.kyoundo.jp](hsugimur@po.kyoundo.jp)  \n4 Department of Oncology, Clinical County Hospital, 540140 Targu Mures, Romania  \n5 Department of Pathology, School of Medicine and Pharmacy, College of Medicine and Health Sciences, University of Rwanda, Kigali P.O. Box 3286, Rwanda; [manirafelix@gmail.com](manirafelix@gmail.com)  \n6 Romanian Academy of Medical Sciences, 030167 Bucures, ti, Romania  \n* Correspondence: simonagurzu@yahoo.com or [simona.gurzu@umfst.ro](simona.gurzu@umfst.ro)  \nAbstract: Mutations and inactivation of the TP53 gene are frequently observed in various types of malignancies. Precise knowledge of the genetic structure and detection of mutation hotspots are crucial, as these indicate a high probability of developing cancer. The aim of our study was to perform the bioinformatic detection of mutation hotspots in the TP53 gene in patients diagnosed with malignant colon neoplasms using self-developed software (version 1) . We compared TP53 gene sequences from 50 healthy individuals with those from 50 patients diagnosed with colorectal carcinoma. Of the 50 samples from cancer patients, the most frequent mutations were observed in exons 5 and 8 (12 mutations per exon) and gene sequences of 12 samples, which differed from those of the 50 samples from healthy individuals. Based on our results, the distribution of mutations in the TP53 gene structure was not even across different exons. By comparing the gene sequences of healthy individuals with those of colon cancer samples, we conclude that structural changes occurring in similar gene regions are not associated with increases in susceptibility to malignancies in every case, namely, that the pathological mechanism is multifactorial.  \nKeywords: TP53 gene; colorectal cancer; bioinformatics; mutation hotspots  \n1. Introduction  \nCancer is a multifaceted disease characterized by uncontrolled cell proliferation, which is often driven by genetic alterations that disrupt critical cellular processes. Mutations and inactivation of the TP53 gene can be observed in several malignancies. Although the direct mechanism is not yet fully understood, we can nevertheless clearly see that, in a physiologically healthy state, this gene plays a crucial role in providing protection for the body by preventing cancerous cell proliferation [1] . Accordingly, gaining precise knowledge of the genetic structures is important, as is screening for the detection of potential mutation hotspots, the presence of which could in","cbCaiavKBYfamTuw","https://ap.wps.com/l/cbCaiavKBYfamTuw","pdf",5540025,12,"English","# Abstract\n# 1. Introduction\n## TP53 as a genome protector\n## Mechanisms of TP53 mutations in cancer","[{\"question\":\"What is the goal of the bioinformatic study on TP53 mutation hotspots?\",\"answer\":\"To detect TP53 gene mutation hotspots in patients diagnosed with malignant colon neoplasms using self-developed software (version 1).\"},{\"question\":\"How were healthy and colorectal cancer samples compared in the study?\",\"answer\":\"TP53 gene sequences from 50 healthy individuals were compared with sequences from 50 patients with colorectal carcinoma.\"},{\"question\":\"Which TP53 exons showed the most frequent mutations in colorectal cancer samples?\",\"answer\":\"The most frequent mutations were observed in exons 5 and 8, with 12 mutations per exon, among the cancer patient samples.\"}]","Bioinformatic Identification of TP53 Gene Mutation Hotspots in Colorectal Cancer | PDF",1789978978]