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Biochemistry 1  \nIntroduction 3  \nExperiment 1 Photometry 15  \nExperiment 2 Chromatography 25  \nExperiment 3 Radioisotope Techniques 45  \nExperiment 4 Electrophoresis 61  \nSection II Proteins and Enzymology 79  \nIntroduction 81  \nExperiment 5 Acid-Base Properties of Amino Acids 105  \nExperiment 6 Sequence Determination of a Dipetide 111  \nExperiment 7 Study of the Properties of ß-Galactosidase 123  \nExperiment 8 Purification of Glutamate Oxaloacetate Transaminase from Pig Heart 135  \nExperiment 9 Kinetic and Regulatory Properties of Aspartate Transcarbamylase 149  \nExperiment 10 Affinity Purificaiton of Glutathione-S-Transferase 157  \nSection III Biomolecules and Biological Systems 163  \nIntroduction 165  \nExperiment 11 Microanalysis of Carbohydrate Mixtures by Isotopic Enzymatic, and Colorimetric Methods 195  \nExperiment 12 Glcose-1-Phosphate: Enzymatic Formation from Starch and Chemical  \nCharacterization 205  \nExperiment 13 Isolation and Characterization of Erythrocyte Membranes 217  \nExperiment 14 Electron Transport 227  \nExperiment 15 Strudy of the Phosphoryl Group Transfer Cascade Goferning Glucose Metabolism  \nAllosteric and Covalent Regulation of Enzyme Activity 243  \nExperiment 16 Experiments in Clinical Biochemistry and Metabolism 253  \nSection IV Immunochemistry 261  \nIntroduction 263  \nExperiment 17 Partial purification of a Polyclonal Antibody, Determination of Titer, and Quantitation of an Antigen Using the ELISA 279  \nExperiment 18 Western Blot to Identify an Antigen 291  \nSection V Nucleic Acids 301  \nIntroduction 303  \nExperiment 19 Isolation of Bacterial DNA 333  \nExperiment 20 Transformation of a genetic Character with Bacterial DNA 339  \nExperiment 21 Constructing and Characterizing a Recombinant DNA Plasmid 345  \nExperiment 22 In Vitro Transcription from a Plasmid Carrying a T7 RNA Polymerase-Specific Promoter 359  \nExperiment 23 In Vitro Translation: mRNA tRNA, and Ribosomes 369  \nExperiment 24 Amplification of a DNA Fragment Using Polymerase Chain Recation 385  \nSection VI Information Science 397  \nIntroduction 399  \nExperiment 25 Obtaining and Analyzing Genetic and Protein Sequence Information via the World  \nWide Web, Lasergene, and RasMol 405  \nAppendix Supplies and Reagents 409  \nIndex 437  \nSECTION I  \nBasic Techniques of  \nExperimental Biochemistry  \nIntroduction  \n􀀂􀀃􀀄􀀅􀀆􀀇􀀈􀀃􀀉􀀊􀀋􀀌 􀀃􀀉 􀀊􀀆􀀇 􀀅􀀆􀀇􀀈􀀃􀀉􀀊􀀋􀀌 􀀄􀀍 􀀎􀀃􀀄􀀏􀀄􀀐􀀃􀀅􀀑􀀏 􀀉􀀌􀀉􀀒􀀊􀀇􀀈􀀉􀀓 􀀂􀀃􀀄􀀏􀀄􀀐􀀃􀀅􀀑􀀏 􀀉􀀌􀀉􀀊􀀇􀀈􀀉 􀀑􀀋􀀇 􀀅􀀄􀀈􀀔􀀏􀀇􀀕􀀖 􀀔􀀄􀀊􀀇􀀗􀀊􀀃􀀑􀀏􀀏􀀌􀀃􀀗􀀘􀀄􀀏􀀘􀀃􀀗􀀐 􀀑 􀀘􀀑􀀋􀀃􀀇􀀊􀀌 􀀄􀀍 􀀄􀀋􀀐􀀑􀀗􀀃􀀉􀀈􀀉􀀖 􀀊􀀃􀀉􀀉􀀙􀀇􀀉􀀖 􀀅􀀇􀀏􀀏 􀀊􀀌􀀔􀀇􀀉􀀖􀀉􀀙􀀎􀀅􀀇􀀏􀀏􀀙􀀏􀀑􀀋 􀀄􀀋􀀐􀀑􀀗􀀇􀀏􀀏􀀇􀀉􀀖 􀀑􀀗􀀚 􀀉􀀔􀀇􀀅􀀃􀀍􀀃􀀅 􀀊􀀌􀀔􀀇􀀉 􀀄􀀍 􀀈􀀄􀀏􀀇􀀒􀀅􀀙􀀏􀀇􀀉􀀓 􀀛􀀄􀀗􀀉􀀇􀀜􀀙􀀇􀀗􀀊􀀏􀀌􀀖 􀀎􀀃􀀄􀀅􀀆􀀇􀀈􀀃􀀉􀀊􀀉 􀀈􀀙􀀉􀀊 􀀉􀀇􀀔􀀑􀀋􀀑􀀊􀀇􀀑􀀗􀀚 􀀉􀀃􀀈􀀔􀀏􀀃􀀍􀀌 􀀊􀀆􀀇􀀉􀀇 􀀉􀀌􀀉􀀊􀀇􀀈􀀉 􀀊􀀄 􀀚􀀇􀀍􀀃􀀗􀀇 􀀑􀀗􀀚 􀀃􀀗􀀊􀀇􀀋􀀔􀀋􀀇􀀊􀀊􀀆􀀇 􀀎􀀃􀀄􀀅􀀆􀀇􀀈􀀃􀀅􀀑􀀏 􀀔􀀋􀀄􀀅􀀇􀀉􀀉 􀀙􀀗􀀚􀀇􀀋 􀀉􀀊􀀙􀀚􀀌􀀓 􀀝􀀄􀀋 􀀇􀀕􀀑􀀈􀀔􀀏􀀇􀀖􀀎􀀃􀀄􀀅􀀆􀀇􀀈􀀃􀀅􀀑􀀏 􀀉􀀊􀀙􀀚􀀃􀀇􀀉 􀀄􀀗 􀀊􀀃􀀉􀀉􀀙􀀇 􀀉􀀏􀀃􀀅􀀇􀀉 􀀄􀀋 􀀞􀀆􀀄􀀏􀀇 􀀄􀀋􀀒􀀐􀀑􀀗􀀃􀀉􀀈􀀉 􀀑􀀋􀀇 􀀍􀀄􀀏􀀏􀀄􀀞􀀇􀀚 􀀎􀀌 􀀉􀀊􀀙􀀚􀀃􀀇􀀉 􀀄􀀗 􀀅􀀇􀀏􀀏􀀙􀀏􀀑􀀋 􀀉􀀌􀀉􀀊􀀇􀀈􀀉􀀓􀀄􀀔􀀙􀀏􀀑􀀊􀀃􀀄􀀗􀀉 􀀄􀀍 􀀅􀀇􀀏􀀏􀀉 􀀑􀀋􀀇 􀀚􀀃􀀉􀀋􀀙􀀔􀀊􀀇􀀚􀀖 􀀉􀀇􀀔􀀑􀀋􀀑􀀊􀀇􀀚􀀖 􀀑􀀗􀀚􀀊􀀆􀀇􀀃􀀋 􀀉􀀙􀀎􀀅􀀇􀀏􀀏􀀙􀀏􀀑􀀋 􀀄􀀋􀀐􀀑􀀗􀀇􀀏􀀏􀀇􀀉 􀀑􀀋􀀇 􀀉􀀊􀀙􀀚􀀃􀀇􀀚􀀓 􀀂􀀃􀀄􀀏􀀄􀀐􀀃􀀅􀀑􀀏􀀈􀀄􀀏􀀇􀀅􀀙􀀏􀀇􀀉 􀀑􀀋􀀇 􀀉􀀊􀀙􀀚􀀃􀀇􀀚 􀀃􀀗 􀀊􀀇􀀋􀀈􀀉 􀀄􀀍 􀀊􀀆􀀇􀀃􀀋 􀀉􀀔􀀇􀀅􀀃􀀍􀀃􀀅􀀈􀀇􀀅􀀆􀀑􀀗􀀃􀀉􀀈􀀉 􀀄􀀍 􀀑􀀅􀀊􀀃􀀄􀀗􀀓 􀀂􀀌 􀀚􀀃􀀘􀀃􀀚􀀃􀀗􀀐 􀀊􀀆􀀇 􀀉􀀌􀀉􀀊􀀇􀀈 􀀙􀀗􀀒􀀚􀀇􀀋 􀀉􀀊􀀙􀀚􀀌 􀀑􀀗􀀚 􀀇􀀏􀀙􀀅􀀃􀀚􀀑􀀊􀀃􀀗􀀐 􀀊􀀆􀀇 􀀑􀀅􀀊􀀃􀀄􀀗 􀀄􀀍 􀀃􀀊􀀉 􀀅􀀄􀀈􀀔􀀄􀀒􀀗􀀇􀀗􀀊 􀀔􀀑􀀋􀀊􀀉􀀖 􀀃􀀊 􀀃􀀉 􀀔􀀄􀀉􀀉􀀃􀀎􀀏􀀇 􀀊􀀄 􀀊􀀆􀀇􀀗 􀀚􀀇􀀍􀀃􀀗􀀇 􀀊􀀆􀀇 􀀍􀀙􀀗􀀅􀀒􀀊􀀃􀀄􀀗 􀀄􀀍 􀀑 􀀔􀀑􀀋􀀊􀀃􀀅􀀙􀀏􀀑􀀋 􀀎􀀃􀀄􀀏􀀄􀀐􀀃􀀅􀀑􀀏 􀀈􀀄􀀏􀀇􀀅􀀙􀀏􀀇 􀀄􀀋 􀀉􀀌􀀉􀀊􀀇􀀈􀀞􀀃􀀊􀀆 􀀋􀀇􀀉􀀔􀀇􀀅􀀊 􀀊􀀄 􀀊􀀆􀀇 􀀅􀀇􀀏􀀏􀀖 􀀊􀀃􀀉􀀉􀀙􀀇􀀖 􀀑􀀗􀀚/􀀄􀀋 􀀄􀀋􀀐􀀑􀀗􀀃􀀉􀀈 􀀑􀀉  \n􀀑 􀀞􀀆􀀄􀀏􀀇􀀓  \n􀀂􀀃􀀄􀀅􀀆􀀇􀀈􀀃􀀅􀀑􀀏 􀀑􀀔􀀔􀀋􀀄􀀑􀀅􀀆􀀇􀀉 􀀊􀀄 􀀊􀀆􀀇 􀀉􀀃􀀈􀀔􀀏􀀃􀀍􀀃􀀅􀀑􀀊􀀃􀀄􀀗􀀑􀀗􀀚 􀀙􀀗􀀚􀀇􀀋􀀉􀀊􀀑􀀗􀀚􀀃􀀗􀀐 􀀄􀀍 􀀎􀀃􀀄􀀏􀀄􀀐􀀃􀀅􀀑􀀏 􀀉􀀌􀀉􀀊􀀇􀀈􀀉 􀀋􀀇􀀜􀀙􀀃􀀋􀀇􀀊􀀞􀀄 􀀊􀀌􀀔􀀇􀀉 􀀄􀀍 􀀎􀀑􀀅k􀀐􀀋􀀄􀀙􀀗􀀚􀀓 􀀝􀀃􀀋􀀉􀀊􀀖 􀀎􀀃􀀄􀀅􀀆􀀇􀀈􀀃􀀉􀀊􀀉 􀀈􀀙􀀉􀀊􀀎􀀇 􀀊􀀆􀀄􀀋􀀄􀀙􀀐􀀆􀀏􀀌 􀀉k􀀃􀀏􀀏􀀇􀀚 􀀃􀀗 􀀊􀀆􀀇 􀀎􀀑􀀉􀀃􀀅 􀀔􀀋􀀃􀀗􀀅􀀃􀀔􀀏􀀇􀀉 􀀑􀀗􀀚􀀊􀀇􀀅􀀆􀀗􀀃􀀜􀀙􀀇􀀉 􀀄􀀍 􀀅􀀆􀀇􀀈􀀃􀀉􀀊􀀋􀀌􀀖 􀀉􀀙􀀅􀀆 􀀑􀀉 􀀉􀀊􀀄􀀃􀀅􀀆􀀃􀀄􀀈􀀇􀀊􀀋􀀌􀀖􀀔􀀆􀀄􀀊􀀄􀀈􀀇􀀊􀀋􀀌􀀖 􀀄􀀋􀀐􀀑􀀗􀀃􀀅 􀀅􀀆􀀇􀀈􀀃􀀉􀀊􀀋􀀌􀀖 􀀄􀀕􀀃􀀚􀀑􀀊􀀃􀀄􀀗 􀀑􀀗􀀚 􀀋􀀇􀀒􀀚􀀙􀀅􀀊􀀃􀀄􀀗􀀖 􀀅􀀆􀀋􀀄􀀈􀀑􀀊􀀄􀀐􀀋􀀑􀀔􀀆􀀌􀀖 􀀑􀀗􀀚 k􀀃􀀗􀀇􀀊􀀃􀀅􀀉􀀓 S􀀇􀀅􀀄􀀗􀀚􀀖􀀎􀀃􀀄􀀅􀀆􀀇􀀈􀀃􀀉􀀊􀀉 􀀈􀀙􀀉􀀊 􀀎􀀇 􀀍􀀑􀀈􀀃􀀏􀀃􀀑􀀋 􀀞􀀃􀀊􀀆 􀀊􀀆􀀇 􀀊􀀆􀀇􀀄􀀋􀀃􀀇􀀉 􀀑􀀗􀀚􀀔􀀋􀀃􀀗􀀅􀀃􀀔􀀏􀀇􀀉 􀀄􀀍 􀀑 􀀞􀀃􀀚􀀇 􀀘􀀑􀀋􀀃􀀇􀀊􀀌 􀀄􀀍 􀀎􀀃􀀄􀀏􀀄􀀐􀀃􀀅􀀑􀀏 􀀑􀀗􀀚 􀀔􀀆􀀌􀀉􀀒􀀃􀀅􀀑􀀏 􀀚􀀃􀀉􀀅􀀃􀀔􀀏􀀃􀀗􀀇􀀉 􀀄􀀍􀀊􀀇􀀗 􀀙􀀉􀀇􀀚 􀀃􀀗 􀀎􀀃􀀄􀀅􀀆􀀇􀀈􀀃􀀅􀀑􀀏 􀀉􀀊􀀙􀀚􀀃􀀇􀀉􀀖􀀉􀀙􀀅􀀆 􀀑􀀉 􀀐􀀇􀀗􀀇􀀊􀀃􀀅􀀉􀀖 􀀋􀀑􀀚􀀃􀀄􀀃􀀉􀀄􀀊􀀄􀀔􀀇 􀀊􀀋􀀑􀀅􀀃􀀗􀀐􀀖 􀀎􀀑􀀅􀀊􀀇􀀋􀀃􀀄􀀏􀀄􀀐􀀌􀀖􀀑􀀗􀀚 􀀇􀀏􀀇􀀅􀀊􀀋􀀄􀀗􀀃􀀅􀀉􀀓 T􀀆􀀃􀀉 􀀗􀀇􀀇􀀚 􀀋􀀇􀀍􀀏􀀇􀀅􀀊􀀉 􀀊􀀆􀀇 􀀎􀀃􀀄􀀅􀀆􀀇􀀈􀀃􀀉􀀊􀀉’􀀋􀀇􀀑􀀚􀀌 􀀑􀀅􀀅􀀇􀀔􀀊􀀑􀀗􀀅􀀇 􀀑􀀗􀀚 􀀙􀀉􀀇 􀀄􀀍 􀀊􀀆􀀇􀀄􀀋􀀃􀀇􀀉 􀀑􀀗􀀚 􀀊􀀇􀀅􀀆􀀗􀀃􀀜􀀙􀀇􀀉􀀍􀀋􀀄􀀈 􀀑􀀏􀀏􀀃􀀇􀀚 􀀑􀀋􀀇􀀑􀀉 􀀑􀀗􀀚 􀀚􀀃􀀉􀀅􀀃􀀔􀀏􀀃􀀗􀀇􀀉􀀓  \nI􀀊 􀀃􀀉 􀀗􀀄􀀊 􀀔􀀄􀀉􀀉􀀃􀀎􀀏􀀇 􀀄􀀋 􀀑􀀔􀀔􀀋􀀄􀀔􀀋􀀃􀀑􀀊􀀇 􀀍􀀄􀀋 􀀊􀀆􀀃􀀉 􀀎􀀄􀀄k 􀀊􀀄􀀉􀀙􀀈􀀈􀀑􀀋","cbCaiaN78RunZhLR","https://ap.wps.com/l/cbCaiaN78RunZhLR","pdf",3548202,425,"English","# Section I Basic Techniques of Experimental Biochemistry\n## Introduction\n## Experiment 1 Photometry\n## Experiment 2 Chromatography\n## Experiment 3 Radioisotope Techniques\n## Experiment 4 Electrophoresis\n# Section II Proteins and Enzymology\n## Introduction\n## Experiment 5 Acid-Base Properties of Amino Acids\n## Experiment 6 Sequence Determination of a Dipetide\n## Experiment 7 Study of the Properties of ß-Galactosidase\n## Experiment 8 Purification of Glutamate Oxaloacetate Transaminase from Pig Heart\n## Experiment 9 Kinetic and Regulatory Properties of Aspartate Transcarbamylase\n## Experiment 10 Affinity Purificaiton of Glutathione-S-Transferase\n# Section III Biomolecules and Biological Systems\n## Introduction\n## Experiment 11 Microanalysis of Carbohydrate Mixtures\n## Experiment 12 Glcose-1-Phosphate: Enzymatic Formation from Starch\n## Experiment 13 Isolation and Characterization of Erythrocyte Membranes\n## Experiment 14 Electron Transport\n## Experiment 15 Phosphoryl Group Transfer Cascade\n## Experiment 16 Experiments in Clinical Biochemistry and Metabolism\n# Section IV Immunochemistry\n## Introduction\n## Experiment 17 ELISA\n## Experiment 18 Western Blot to Identify an Antigen\n# Section V Nucleic Acids\n## Introduction\n## Experiment 19 Isolation of Bacterial DNA\n## Experiment 20 Transformation of a genetic Character with Bacterial DNA\n## Experiment 21 Constructing and Characterizing a Recombinant DNA Plasmid\n## Experiment 22 In Vitro Transcription from a Plasmid\n## Experiment 23 In Vitro Translation\n## Experiment 24 Amplification of a DNA Fragment Using Polymerase Chain Recation\n# Section VI Information Science\n## Introduction\n## Experiment 25 Obtaining and Analyzing Genetic and Protein Sequence Information\n# Appendix and Index\n## Appendix Supplies and Reagents\n## Index","[{\"question\":\"该文档的实验内容如何分段组织？\",\"answer\":\"文档按 Section I 至 Section VI 进行分段组织，每个部分包含 Introduction，并列出若干具体实验及其对应页码范围。\"},{\"question\":\"Section I 重点包含哪些基础实验技术？\",\"answer\":\"Section I 包含 Photometry、Chromatography、Radioisotope Techniques 和 Electrophoresis 等实验，面向实验生物化学的基础方法训练。\"},{\"question\":\"后续部分如何从免疫到核酸与信息科学展开？\",\"answer\":\"Section IV 给出 ELISA 与 Western blot 等免疫化学实验；Section V 进一步涵盖细菌 DNA 的分离、转化、重组质粒构建、体外转录/翻译与 PCR 扩增；Section VI 则转向通过网络与相关软件获取并分析遗传与蛋白序列信息。\"}]","Basic Techniques of Experimental Biochemistry | PDF",1789975923,1071]