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This manual, which explains the basic techniques necessary to carry out microbiology experiments safely and effectively, is intended as a guide for teachers and technicians.  \nThe Microbiology Society, in association with the Microbiology in Schools Advisory Committee (MiSAC) has also produced a book of 21 practical investigations which complements the manual – Practical Microbiology for Secondary Schools is suitable for use with Key Stages 3, 4 and post-16 and the equivalent Scottish qualifications. This resource can be downloaded from the Microbiology Society’s dedicated [education website: www.microbiologyonline.org.uk](education website: www.microbiologyonline.org.uk)  \nThe Microbiology Society offers schools membership and runs an advice service on microbiology teaching. For further details, email [education@microbiologysociety.org](education@microbiologysociety.org), telephone +44 (0)20 7685 2682 or write to the address at the bottom of the page.  \nOther useful sources of advice:  \nx CLEAPSS – [www.cleapss.org.uk](www.cleapss.org.uk)  \nThe Gardiner Building, Brunel Science Park, Uxbridge UB8 3PQ  \nx Microbiology in Schools Advisory Committee (MiSAC)– [www.misac.org.uk](www.misac.org.uk)[ ](www.misac.org.uk)[c/o NCBE](c/o NCBE) (see address below)  \nx National Centre for Biotechnology Education (NCBE)– [www.ncbe.reading.ac.uk](www.ncbe.reading.ac.uk)[ ](www.ncbe.reading.ac.uk)Science and Technology Centre, The University of Reading, 2 Earley Gate, Whiteknights, Reading RG6 6AU  \nx Scottish Schools Equipment Research Centre (SSERC)– [www.sserc.org.uk](www.sserc.org.uk)  \n2 Pitreavie Court, South Pitreavie Business Park, Dunfermline KY11 8UB  \nCredits & Acknowledgements  \nThe Microbiology Society gratefully acknowledges the support from the following sources:  \nx Kath Crawford (SAPS Scotland) x John Richardson (SSERC)  \nx Members of MiSAC x John Schollar (NCBE)  \nx John Tranter (CLEAPSS)  \nEditors: Dariel Burdass, John Grainger & Janet Hurst  \nDesign and Production Editor: Ian Atherton  \nPhotographs: Dariel Burdass & Faye Jones  \nFront cover illustration: Wladimir Bulgar/Science Photo Library ISBN 0 95368 383 4  \nCopyright  \nBasic Practical Microbiology – A Manual is copyright. The Microbiology Society asserts its moral right to be identified as copyright holder under Section 77 of the Designs, Patents and Copyright Act (UK) 1988.  \nEducational use: Electronic or paper copies of the resource or individual pages from it may be made for classroom and bona fide educational uses, provided that the copies are distributed free of charge or at the cost of reproduction and that the Microbiology Society is credited and identified as copyright holder.  \nPublished by the Microbiology Society, Charles Darwin House, 12 Roger Street, London WC1N 2JU, UK  \n© 2016 Microbiology Society  \nContents  \nAbout this resource Inside front cover  \nPart 1: The Basics  \nAn introduction to microbiology, aseptic technique and safety  \nPreparation  \nSafety guidelines 1  \nRisk assessment 2  \nGood microbiological laboratory practice (GMLP) 3  \nSpillage management 3  \nAerosols 3  \nResources  \nEquipment 4  \nApparatus 5  \nMaterials 5  \nMedia, sterilisation and disinfection  \nPreparation of culture media 6  \nPouring a plate 6  \nStorage of media 6  \nSterilisation vs disinfection 6  \nSterilisation using the autoclave/pressure cooker 7  \nSterilisation of equipment and materials 7  \nChoice, preparation and use of disinfectants 7  \nInoculation and other aseptic procedures  \nEssential points 8  \nUsing a wire loop 8  \nUsing a pipette 9  \nFlaming the neck of bottles and test tubes 10  \nWorking with bacteria and yeast  \nStreak plate 11  \nPour plate 12  \nUsing a spreader 13  \nSpread plate 14  \nWorking with moulds 15  \nIncubation 16  \nIn conclusion: clearing up 17  \nEssential methods for maintaining, preparing and using cu","cbCaisHhdtEtOqIF","https://ap.wps.com/l/cbCaisHhdtEtOqIF","pdf",2776132,46,"English","# About this resource\n# Part 1: The Basics\n## Introduction to microbiology, aseptic technique and safety\n## Preparation\n## Resources\n## Media, sterilisation and disinfection\n## Inoculation and other aseptic procedures\n## Working with bacteria and yeast\n## Working with moulds\n## Essential methods for maintaining, preparing and using cultures\n# Part 2: Microbiology in Action\n## Practical activities\n## Microscopy\n# Appendices","[{\"question\":\"Who is this manual intended for and what is its purpose?\",\"answer\":\"The manual is intended as a guide for teachers and technicians. Its purpose is to provide practical microbiology techniques so investigations proceed safely and achieve educational aims successfully.\"},{\"question\":\"What safety and compliance topics are covered in the preparation section?\",\"answer\":\"It addresses safety guidelines and notes that activities involving micro-organisms are controlled by COSHH Regulations, with duties under the Health and Safety at Work Act.\"},{\"question\":\"Which laboratory topics are included in Part 1, and what practical activities appear in Part 2?\",\"answer\":\"Part 1 covers introduction, aseptic technique, risk and safety, equipment and materials, media preparation, sterilisation versus disinfection, and aseptic inoculation plus culture maintenance. Part 2 includes practical activities such as antimicrobial sensitivity testing and microscopy methods.\"}]","Basic Practical Microbiology - A Manual - Teaching and laboratory safety guide | PDF",1790931437,116]