[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-398699-105":59,"doc-detail-398699-en":131},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":124,"head_meta":126,"extra_data":128,"updated_unix":130},105,"en","bacterial-identification-the-essentials-for-plate-round","Bacterial Identification - The Essentials for Plate Round","","Bacterial identification procedures focus on diagnosing bacterial infections using plate-based screening and classic laboratory tests. The workflow starts with Gram staining and assessment on three plates, then distinguishes Gram-positive cocci by morphology and key biochemical reactions such as catalase and coagulase. The guide details methods to separate Staphylococci and Streptococci, including hemolysis patterns, bacitracin (A-disc) susceptibility, PYR testing, optochin susceptibility for Streptococcus pneumoniae, and bile esculin for Group D characterization, followed by CAMP and Hippurate hydrolysis. It also addresses identification of Group C, F, G and differentiation within Group B and non-beta hemolytic groups.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/bacterial-identification-the-essentials-for-plate-round/398699/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/bacterial-identification-the-essentials-for-plate-round/398699.png","ImageObject",300,407,{"name":92,"@type":93},"supergirl","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-30","2026-09-27",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",16,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What is the starting workflow for identifying bacteria in this guide?","Question",{"text":113,"@type":114},"Begin with Gram staining and culture on three plates, then use morphology and targeted tests (e.g., catalase/coagulase, hemolysis assessment, and susceptibility or biochemical assays) to narrow down the organism groups.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How are Staphylococci differentiated from other Gram-positive cocci?",{"text":118,"@type":114},"Use Gram stain morphology and a catalase test, then apply coagulase testing to distinguish S. aureus from coagulase-negative staphylococci; Novobiocin susceptibility helps separate S. saprophyticus from other coagulase-negative species.",{"name":120,"@type":111,"acceptedAnswer":121},"Which tests help identify Streptococcus pneumoniae and Group D organisms?",{"text":122,"@type":114},"Identify S. pneumoniae with an Optochin (P-disc) test, and if that is negative use bile esculin (esculin hydrolysis) to demonstrate Group D streptococci; further differentiation can use NaCl growth and PYR testing.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},398699,1790527789,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":145,"read_time":146,"preview_page_count":4},962088121634,"https://ap-avatar.wpscdn.com/davatar_9964176cb1d06d4a9deccf72a44ae3dc","01/ 12/54  \nBacterial Identification The Essentials for Plate Round  \nRongpong Plongla, MD. MSc.  \nOct 07, 2011  \n[www.idchula.org](www.idchula.org)  \nDiagnosis of Bacterial Infection  \n01/ 12/54  \nIdentification of Bacteria  \nMorphology Growth requirements Biochemistry Enzymes  \nAntigens  \nAlways Starting with Gram Staining and 3 Plates  \n01/ 12/54  \nGram-Positive Cocci  \n• 1. differentiate Staphylococci VS Streptococci  \n– Using Gram stain morphology  \n• Staphylococci: in cluster  \n• Streptococci: in pair or in chain  \n• *Micrococci: in quadruplets/Tetrads with lemonyellow colonies  \n– Catalase test  \n• Staphylococci: positive  \n• Streptococci: negative  \n• This enzyme is responsible for protecting bacteria from hydrogen peroxide (H2O2) accumulation, which can occur during aerobic metabolism.  \n• Catalase breaks H2O2 down into water and O2  \n• * most aerobic organism make catalase i.e Staphylococci, Micrococci, Rothia, Pseudomonas aeruginosa, Serratia marcescens, Aspergillus, Nocardia, Burkhodelria cepacea  \nStaphylococcus  \n• 1. looking at Coagulase test to distinguish S. aureus  \n– Positive: S. aureus  \n• S. aureus colonies is usually yellow.  \n– Negative: Coagulase-Negative Staphylococci  \n01/ 12/54  \nStaphylococcus  \n• 2. distinguish S.saprophyticus from other Coagulase-negative Staphylococci (i.e. S. epidermidis) with Novobiocin Susceptibility  \n– S. saprophyticus: resistant to novobiocin  \n– S. epidermidis: sensitive to novobiocin  \nS. saprophyticus caused UTI  \nStreptococci  \n• 1. looking at hemolysis on horse/sheep blood agar, then grouping into beta vs non-beta hemolysis  \nBeta: complete hemolysis-transparent  \nAlpha: partial hemolysis-greenish  \nGamma: no hemolysis  \n01/ 12/54  \nBeta-hemolysis Streptococci  \n• 1. Streptococcus Group A (S.pyogenes)  \n• 2. Streptococcus Group B (S.agalactiae)  \n• 3. Streptococcus Group C, F, G  \nB eta-hemolytic Streptococci  \n• 1. looking at A-disc (Bacitracin disc) for Streptococcus pyogenes (group A): GAS  \n– Streptococcus group A : sensitive to bacitracin  \n– Also PYR positive  \n01/ 12/54  \nBeta-hemolytic Streptococci  \n• 2. if A-disc is negative, identify Group B  \nStreptococcus using 2 methods  \n– 1. CAMP test  \n– 2. Hippurate Hydrolysis  \n+ -  \nChristie-Atkins, Munch-Petersen (CAMP) test  \n• Detects the production of enhanced hemolysis that occurs when b-lysin and the hemolysins of Group B streptococci come in contact on sheep blood agar  \n• Organisms that positive CAMP test  \n– GBS  \n– Listeria ivanovii  \n+ -  \nGroup B streptococci showing the classical “arrow-shaped hemolysis near the staphylococcus streak  \n– Rhodococcus equi  \n01/ 12/54  \nReverse CAMP test  \nPositive and negative results.  \nGBS is streaked down the center of the plate the test organisms, Clostridium sp., are streaked at right angles to the GBS.  \n• Positive reaction of C.perfringens:“bow-tie\" zone of hemolysis.  \n•Alpha toxin + CAMP factor  \n• Negative reaction of C. septicum:  \nshows no hemolysis.  \nBeta-hemolytic Streptococci  \n• If A-disc, Hippurate test and CAMP test are negative  \n􀃆Streptococcus Group C, F, G  \n􀃆If you want to know the species (which almost not necessary): anti-sera or API  \n01/ 12/54  \nNon-beta hemolytic Streptococci  \n• Streptococcus pneumoniae  \n• Group D Streptococci  \n– Enterococci  \n– S. bovis  \n• Viridans Streptococci  \n– anginosus group (formerly milleri group: small  \ncolonies and small, round shape gram positive bacteria )  \n– sanguinis group  \n– mitis group  \n– mutans group  \nNon-beta hemolytic Streptococci  \n• 1. using P-disc (Optochin disc) to identify S.pneumoniae  \n**note that  \n1. Lancet shaped diplococci with capsule are quite unique for S. pneumoniae  \n2. S.pneumoniae colonies can be mucoid, flat, and umbilicated  \nIf it’s S.pneumoniae There’re the MICs issues about whether meningeal or nonmeningeal isolates  \nAzone of inhibition of 14 mm. or more in diameter will be seen around the disk after incubation if the organism is Streptococcus pneumoniae.  \nOther alpha-hemol","cbCaig9FY8et3zpV","https://ap.wps.com/l/cbCaig9FY8et3zpV","pdf",2767674,25,"English","# Diagnosis of Bacterial Infection\n## Identification of Bacteria\n## Gram-Positive Cocci\n## Staphylococcus\n## Streptococci\n## Beta-Hemolysis Streptococci\n## Non-beta Hemolytic Streptococci\n## PYR test","[{\"question\":\"What is the starting workflow for identifying bacteria in this guide?\",\"answer\":\"Begin with Gram staining and culture on three plates, then use morphology and targeted tests (e.g., catalase/coagulase, hemolysis assessment, and susceptibility or biochemical assays) to narrow down the organism groups.\"},{\"question\":\"How are Staphylococci differentiated from other Gram-positive cocci?\",\"answer\":\"Use Gram stain morphology and a catalase test, then apply coagulase testing to distinguish S. aureus from coagulase-negative staphylococci; Novobiocin susceptibility helps separate S. saprophyticus from other coagulase-negative species.\"},{\"question\":\"Which tests help identify Streptococcus pneumoniae and Group D organisms?\",\"answer\":\"Identify S. pneumoniae with an Optochin (P-disc) test, and if that is negative use bile esculin (esculin hydrolysis) to demonstrate Group D streptococci; further differentiation can use NaCl growth and PYR testing.\"}]","Bacterial Identification - The Essentials for Plate Round | PDF",1790488210,63]