[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-detail-450180-en":59,"doc-seo-450180-105":81},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":5,"data":60},{"doc_id":61,"user_id":62,"nickname":63,"user_avatar":64,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":66,"doc_content":67,"file_id":68,"file_url":69,"file_type":70,"file_size":71,"view_count":72,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":29,"language":73,"language_code":74,"site_id":75,"html_lang":74,"table_of_contents":76,"faqs":77,"seo_title":78,"seo_description":66,"update_tm":79,"read_time":80,"preview_page_count":4},450180,8796095461564,"Liam","https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d","Backbone resonance assignment of the pentraxin domain of the neuronal pentraxin receptor (NPTXRPTX) - Abstract","Neuronal pentraxin receptor (NPTXR) is a synaptic organizing protein crucial for excitatory neurotransmission, but its structural features are insufficiently defined. The conserved C-terminal pentraxin (PTX) domain (NPTXRPTX) is thought to mediate interactions with synaptic partners, yet lacks structural characterization. The study provides near-complete backbone NMR resonance assignments for uniformly 15N/13C-labeled NPTXRPTX, enabling future analyses of NPTXR–ligand interactions and informing molecular mechanisms of synaptic assembly and maintenance.","Biomolecular NMR Assignments (2026) 20:10 [https://doi.org/10.1007/s12104-025-10259-w](https://doi.org/10.1007/s12104-025-10259-w)  \nARTICLE  \nBackbone resonance assignment of the pentraxin domain of the neuronal pentraxin receptor (NPTXR)  \nAlexandra K. Pozhidaeva1 · Yunfeng Li1 · Yulia Pustovalova1 · Jeffrey C. Hoch1 · David C. Martinelli2 · Bernd Simon1 · Bing Hao1  \nReceived: 4 December 2025 / Accepted: 28 December 2025 © The Author(s) 2026, modified publication 2026  \nAbstract  \nNeuronal pentraxin receptor (NPTXR) is a synaptic organizing protein important for excitatory neurotransmission, yet its structural properties remain poorly defined. The conserved C-terminal pentraxin (PTX) domain of NPTXR (NPTXRPTX) is expected to mediate interactions with synaptic partners but has not been structurally characterized. Here, we report nearcomplete backbone NMR resonance assignments of NPTXRPTX using uniformly 15N, 13C-labeled protein. These assignments provide a foundation for further studies of NPTXR-ligand interactions that drive NPTXR-dependent synapse organization and will advance our understanding of the molecular mechanisms underlying synaptic assembly and maintenance.  \nKeywords Long pentraxins · Neuronal pentraxins · NPR · Solution NMR  \nBiological context  \nNeuronal pentraxin receptor (NPTXR) is a type II transmembrane protein of the neuronal pentraxin (NP) family, which also includes neuronal pentraxin 1 (NPTX1) and neuronal pentraxin 2 (NPTX2, also known as Narp) . Members of the NP family were first identified based on their ability to bind the snake venom toxin taipoxin (Schlimgen et al. 1995; Dodds et al. 1997) . They are evolutionarily related to classical immune pentraxins such as C-reactive protein (CRP) and serum amyloid P component (SAP), but have acquired specialized roles in the nervous system (Doddset al. 1997) . Together, they play important roles in synapse formation, remodeling, and elimination, thereby regulating synaptic transmission, plasticity, and behavior (Xu et al. 2003; Bjartmar et al. 2006; Sia et al. 2007; Lee et al. 2017) .  \n􀀍 Bernd Simon [bsimon@uchc.edu](bsimon@uchc.edu)  \n􀀍 Bing Hao [bhao@uchc.edu](bhao@uchc.edu)  \n1 Department of Molecular Biology and Biophysics, University of Connecticut Health Center, Farmington CT 06030, USA  \n2 Department of Neuroscience, University of Connecticut Health Center, Farmington, CT 06030, USA  \nNPTXRis produced predominantly inexcitatory neurons, where it localizes to presynaptic membranes (Kirkpatrick et al. 2000; Sia et al. 2007; Sticco et al. 2021) . When coexpressed with other NP proteins in the same cell, NPTXR acts as a central hub to form heteromeric complexes with the secreted NP paralogs NPTX1 and NPTX2, tethering them to the presynaptic membrane surface to fine-tune their synaptic function (Kirkpatrick et al. 2000; Lee et al. 2017) . Loss of NPTXR results in reduced levels of NPTX1 and NPTX2 in vivo, suggesting that NPTXR contributes to their trafficking and/or stability (Bjartmar et al. 2006) . Through direct or indirect (via NPTX1 and NPTX2) interactions with AMPA-type glutamate receptors, NPTXR promotes AMPA receptor clustering and surface presentation, and subsequently strengthens synaptic transmission (Sia et al. 2007; Lee et al. 2017) . Additional binding partners, including trans-synaptic organizer C1Q-like 3 protein, have further linked NPTXR to synapse assembly and behavior (Martinelli et al. 2016; Chew et al. 2017; Sticco et al. 2021; Caro et al. 2025) . Consistent with its role as a synaptic signaling hub, altered NPTXR expression has been associated with neuropsychiatric and neurodegenerative disorders, including schizophrenia, Alzheimer’s disease, and age-associated cognitive decline (Begcevic et al. 2018; Lim et al. 2020; Dulewicz et al. 2021; Gomez de San Jose et al. 2022) .  \nThe NP family proteins are defined by a characteristic C-terminal pentraxin (PTX) domain of approximately 200  \n1 3  \namino acids, which mediates ligand recogn","cbCaictMRszZMTlk","https://ap.wps.com/l/cbCaictMRszZMTlk","pdf",1461234,3,"English","en",105,"# Abstract\n# Biological context\n# Methods and experiments\n## Construct design\n## Sample preparation","[{\"question\":\"Why are backbone resonance assignments important for NPTXRPTX?\",\"answer\":\"They establish sequence-specific NMR reference points for subsequent structural and interaction studies of the PTX domain’s role in synaptic organization.\"},{\"question\":\"What protein region was studied in the assignments?\",\"answer\":\"The conserved C-terminal pentraxin (PTX) domain of NPTXR, termed NPTXRPTX, corresponding to residues 273–493.\"},{\"question\":\"How was NPTXRPTX prepared for NMR?\",\"answer\":\"NPTXRPTX was expressed using uniformly 15N/13C labeling in E. coli SHuffle cells, with the PTX-encoding region cloned into a modified pET32a vector and purified after TEV cleavage.\"}]","Backbone resonance assignment of the pentraxin domain of the neuronal pentraxin receptor (NPTXRPTX) - Abstract | PDF",1790732363,15,{"code":4,"msg":82,"data":83},"ok",{"site_id":75,"language":74,"slug":84,"title":65,"keywords":85,"description":66,"schema_data":86,"social_meta":139,"head_meta":141,"extra_data":143,"updated_unix":144},"backbone-resonance-assignment-of-the-pentraxin-domain-of-the-neuronal-pentraxin-receptor-nptxrptx-abstract","",{"@graph":87,"@context":138},[88,101,121],{"@type":89,"itemListElement":90},"BreadcrumbList",[91,95,97,99],{"item":92,"name":93,"@type":94,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":96,"name":9,"@type":94,"position":14},"https://docshare.wps.com/document/",{"item":98,"name":35,"@type":94,"position":72},"https://docshare.wps.com/document/healthcare/",{"item":100,"name":65,"@type":94,"position":19},"https://docshare.wps.com/document/backbone-resonance-assignment-of-the-pentraxin-domain-of-the-neuronal-pentraxin-receptor-nptxrptx-abstract/450180/",{"url":100,"name":65,"@type":102,"image":103,"author":108,"headline":65,"publisher":110,"fileFormat":113,"inLanguage":74,"description":66,"dateModified":114,"datePublished":115,"encodingFormat":113,"isAccessibleForFree":116,"interactionStatistic":117},"DigitalDocument",{"url":104,"@type":105,"width":106,"height":107},"https://docshare.wps.com/thumbnails/backbone-resonance-assignment-of-the-pentraxin-domain-of-the-neuronal-pentraxin-receptor-nptxrptx-abstract/450180.png","ImageObject",300,407,{"name":63,"@type":109},"Person",{"url":92,"name":111,"@type":112},"DocShare","Organization","application/pdf","2026-11-06","2026-09-30",true,{"@type":118,"interactionType":119,"userInteractionCount":72},"InteractionCounter",{"@type":120},"ViewAction",{"@type":122,"mainEntity":123},"FAQPage",[124,130,134],{"name":125,"@type":126,"acceptedAnswer":127},"Why are backbone resonance assignments important for NPTXRPTX?","Question",{"text":128,"@type":129},"They establish sequence-specific NMR reference points for subsequent structural and interaction studies of the PTX domain’s role in synaptic organization.","Answer",{"name":131,"@type":126,"acceptedAnswer":132},"What protein region was studied in the assignments?",{"text":133,"@type":129},"The conserved C-terminal pentraxin (PTX) domain of NPTXR, termed NPTXRPTX, corresponding to residues 273–493.",{"name":135,"@type":126,"acceptedAnswer":136},"How was NPTXRPTX prepared for NMR?",{"text":137,"@type":129},"NPTXRPTX was expressed using uniformly 15N/13C labeling in E. coli SHuffle cells, with the PTX-encoding region cloned into a modified pET32a vector and purified after TEV cleavage.","https://schema.org",{"og:url":100,"og:type":140,"og:title":65,"og:site_name":111,"og:description":66},"article",{"robots":142,"canonical":100},"index,follow",{"doc_id":61,"site_id":75},1792879034]