[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-354971-105":59,"doc-detail-354971-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","b7-h3-and-gd2-overexpression-as-immunotherapeutic-targets-in-retinoblastoma","B7-H3 and GD2 overexpression as immunotherapeutic targets in retinoblastoma","","Retinoblastoma (RB) is the most common malignant eye tumor in children, and improved immunotherapeutic strategies require reliable tumor-specific surface antigens. This study evaluates expression patterns of tumor antigens and immune checkpoint proteins in primary and secondary enucleated RB samples, then examines associations with clinical and pathological data. Immunohistochemical analysis was performed on 94 FFPE RB specimens from Thai patients, assessing B7-H3, GD2, CD171, and PD-L1.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/b7-h3-and-gd2-overexpression-as-immunotherapeutic-targets-in-retinoblastoma/354971/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/b7-h3-and-gd2-overexpression-as-immunotherapeutic-targets-in-retinoblastoma/354971.png","ImageObject",300,407,{"name":92,"@type":93},"Emma Wilson","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-30","2026-09-22",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What was the main purpose of evaluating B7-H3, GD2, CD171, and PD-L1 in retinoblastoma?","Question",{"text":112,"@type":113},"To identify and characterize tumor antigen and immune checkpoint expression in RB samples and to explore how these expressions relate to clinical and pathological features.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were the antigens and immune checkpoint proteins measured in this study?",{"text":117,"@type":113},"By immunohistochemical analysis of 94 formalin-fixed paraffin-embedded retinoblastoma tissue samples.",{"name":119,"@type":110,"acceptedAnswer":120},"Which target proteins showed the highest and lowest expression frequencies?",{"text":121,"@type":113},"B7-H3 showed the highest expression (51.06%), while PD-L1 was the least expressed (4.26%) in the analyzed samples.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},354971,1790740914,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},3848291630094,"https://eur-avatar.wpscdn.com/davatar_085a072bc5b1113ac321206ff7593b45","[www. nature.com/scientificreports](www. nature.com/scientificreports)  \nOPEN  \nB7-H3 and GD2 overexpression as immunotherapeutic targets inretinoblastoma  \nJatuporn Sujjitjoon1,2,6, Wei Loon Ng3,6, Katesara Kongkla1,2, Kridtin Jarutatsanangkoon4, Krittiya Chongsutakawewong3, Subongkoch Subhadhirasakul3, Mongkol Uiprasertkul4, Suthipol Udompunturak5, Mutita Junking1,2, Pa-thai Yenchitsomanus1,2 &  \nLa-ongsri Atchaneeyasakul3􀀍  \nRetinoblastoma (RB) is the most prevalent malignant eye tumor in children. Chimeric antigen receptor T-cell therapy showed significant promise in treating blood cancers and could potentially be effective  \nfor RB. A critical step toward advancing this therapy involves identifying surface antigens on RB cells. This study focuses on evaluating the expression of tumor antigens and immune checkpoint proteins in primary and secondary enucleated RB samples and analyzing their relationship with clinical and pathological data. We performed immunohistochemical analysis on 94 formalin-fixed, paraffinembedded RB tissue samples from Thai patients to assess the expression of B7-H3, GD2, CD171, and PD-L1. B7-H3 was the most frequently expressed antigen, appearing in 51.06% of samples (48/94) with mild to moderate membranous intensity. GD2 was present in 36.17% of samples (34/94) with moderate staining intensity. CD171 was detected in 9.57% of samples (9/94), showing weak to mild membranous intensity. PD-L1 was the least expressed, found in only 4.26% of samples (4/94) with mild to moderate membrane staining. There were no significant correlations between the expression of these antigens and patient characteristics. Our study emphasizes the variability in tumor antigen expression in RB. The notable expression of B7-H3 and GD2 suggests they could be promising targets for immunotherapy. These findings encourage further investigation into multi-specific or combination immunotherapies for treating retinoblastoma.  \nKeywords Retinoblastoma, B7-H3, Cancer immunotherapy, CD171, GD2, PD-L1  \nAbbreviations  \nB7-H3 B7 Homolog 3  \nCAR Chimeric antigen receptor  \nCD171 Cluster of Differentiation 171  \nFFPE Formalin-fixed paraffin-embedded GD2 Disialoganglioside  \nIHC Immunohistochemistry  \nL1CAM L1 cell adhesion molecule  \nMHC Major histocompatibility complex PD-l Programmed death-1  \nPD-L1 Programmed death ligand-1  \nRB Retinoblastoma  \nRetinoblastoma (RB) is the most common intraocular malignant tumor in children1. The primary goals in managing RB are to save the patient’s life and prevent metastasis, followed by preserving the affected eye and, if possible, restoring vision. Although advance treatments for retinoblastoma include intra-ophthalmic artery and  \n1Siriraj Center of Research Excellence for Cancer Immunotherapy (SiCORE-CIT), Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. 2Division of Molecular Medicine, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. 3Department of Ophthalmology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. 4Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. 5Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. 6Jatuporn Sujjitjoon and Wei Loon Ng contributed equally to this work.  \n􀀍 email: [atchanee@hotmail.com](atchanee@hotmail.com); [laongsri.ath@mahidol.ac.th](laongsri.ath@mahidol.ac.th)  \n[www. nature.com/scientificreports/](www. nature.com/scientificreports/)  \nintravitreal chemotherapy were used2,3, patients in developing countries often present with metastatic or recurrent disease, leading to poor outcomes. New treatment strategies are needed to address chemo-resistant or advanced retinoblastoma, aiming to preserve the eye and minimize the side effects of high-dose systemic chemotherapy, particularly the risk of secondary cancers. Targeted immunotherapy, employing both antibodies a","cbCailXxNiVhfnuj","https://ap.wps.com/l/cbCailXxNiVhfnuj","pdf",4551410,14,"English","# Abstract\n## Study objectives and rationale\n## Methods (IHC cohort and targets)\n## Key findings (expression frequencies)\n## Clinical implications and future directions","[{\"question\":\"What was the main purpose of evaluating B7-H3, GD2, CD171, and PD-L1 in retinoblastoma?\",\"answer\":\"To identify and characterize tumor antigen and immune checkpoint expression in RB samples and to explore how these expressions relate to clinical and pathological features.\"},{\"question\":\"How were the antigens and immune checkpoint proteins measured in this study?\",\"answer\":\"By immunohistochemical analysis of 94 formalin-fixed paraffin-embedded retinoblastoma tissue samples.\"},{\"question\":\"Which target proteins showed the highest and lowest expression frequencies?\",\"answer\":\"B7-H3 showed the highest expression (51.06%), while PD-L1 was the least expressed (4.26%) in the analyzed samples.\"}]","B7-H3 and GD2 overexpression as immunotherapeutic targets in retinoblastoma | PDF",1790115186,35]