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Integrated analyses across TCGA and GEO datasets evaluated expression, prognosis, and mutation-related epigenetic features. Correlations with immune cell infiltration and pathway activity were assessed, followed by lentiviral overexpression and B4GALNT1 knockdown in HepG2 cells to measure proliferation and apoptosis.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/b4galnt1-regulates-hepatocellular-carcinoma-cell-proliferation-and-apoptosis-via-the-pi3k-akt-mtor-pathway/364562/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/b4galnt1-regulates-hepatocellular-carcinoma-cell-proliferation-and-apoptosis-via-the-pi3k-akt-mtor-pathway/364562.png","ImageObject",300,407,{"name":92,"@type":93},"Noah","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-02","2026-09-23",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",12,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What is the main focus of this study on B4GALNT1 in hepatocellular carcinoma?","Question",{"text":113,"@type":114},"The study investigates whether B4GALNT1 regulates HCC cell proliferation and apoptosis and how it relates to prognosis and immune-related features via the PI3K–AKT–mTOR pathway.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How were dataset analyses used to evaluate B4GALNT1 in HCC?",{"text":118,"@type":114},"Expression differences were compared across TCGA_LIHC and GEO datasets, while prognosis was assessed using Cox regression. Mutation links to CpG island methylation and outcomes were examined using cBioPortal and MethSurv.",{"name":120,"@type":111,"acceptedAnswer":121},"What experimental approach was used to test the functional role of B4GALNT1?",{"text":122,"@type":114},"A lentiviral vector expressing B4GALNT1 was constructed, and B4GALNT1 was knocked down in HepG2 cells. Cell proliferation assays and flow cytometry were then used to measure proliferation and apoptosis effects.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},364562,1790785211,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":145,"read_time":146},8796095462418,"https://ap-avatar.wpscdn.com/avatar/80000253c1241d02b47?x-image-process=image/resize,m_fixed,w_180,h_180&k=1778826106357471780","Journal of Clinical Laboratory Analysis  \nRESEARCH ARTICLE  OPEN ACCESS   \nB4GALNT1 Regulates Hepatocellular Carcinoma Cell Proliferation and Apoptosis via the  \nPI3K–AKT–mTOR Pathway  \nLihan Bie1 | Guangquan Chen2 | Xin Lei1 | Feng Xiao3 | Zheng Xu1 | Zhouhong Xiang1 | Zhicheng Lu1 | Xiudi Jiang1   \n1Department of Laboratory Medicine, The Seventh People's Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China | 2Shanghai Key Laboratory of Maternal-Fetal Medicine, Department of Fetal Medicine and Prenatal Diagnosis Center, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai, China | 3Department of Pathology, The Seventh People's Hospital Affiliated to the  \nShanghai University of Traditional Chinese Medicine, Shanghai, China Correspondence: Xiudi Jiang ([xiu202401@163.com](xiu202401@163.com))  \nReceived: 1 September 2024 | Revised: 15 December 2024 | Accepted: 8 January 2025  \nFunding: This research was funded by the Shanghai Pudong Commission of Health and Family Planning (No. PW2023A-32), the Science and Technology Development Fund of Shanghai Pudong New Area (No. PKJ2023-Y02), the Talents Training Program of the Seventh People's Hospital, Shanghai University of Traditional Chinese Medicine (Grant No. QMX2022-05), and the National Natural Science Foundation of China (No. 82203999) .  \nKeywords: B4GALNT1 | HCC | immune cell infiltration | immune checkpoint genes | PI3K–AKT–mTOR pathway  \nABSTRACT  \nBackground: Hepatocellular carcinoma (HCC) is a ubiquitous malignancy linked to significant mortality. The abnormal expression of β-1,4-N-acetyl-galactosaminyltransferase 1 (B4GALNT1) seemed to be implicated in tumorigenesis. Nonetheless, this enzyme's roles in HCC are unclear.  \nMethods: By analyzing the TCGA_LIHC, GSE77509, and GSE135631 datasets, the levels of B4GALNT1 expression in HCC and surrounding non-cancerous tissues were compared. The prognostic implications of B4GALNT1 were assessed using the Cox regression analysis (CRA) . The relationship of B4GALNT1 mutations with CpG island methylation levels and prognosis was examined by analyzing the cBioPortal and MethSurv databases. We sifted the evidence of B4GALNT1 expression correlating with 28 immune cell types' infiltration by harnessing the “GSVA” R package. To delve into the influences of genes associated with B4GALNT1 on HCC, we implemented gene set enrichment analysis (GSEA) . We constructed a lentiviral vector expressing B4GALNT1 and knocked down B4GALNT1 in HepG2 cells. The resulting effects on HCC cell proliferation and apoptosis were analyzed via cell proliferation assays and flow cytometry.  \nResults: HCC tissues presented significant B4GALNT1 overexpression relative to surrounding non-cancerous tissues, marking it as a standalone risk factor for HCC progression. Methylation levels of two CpG islands were high, suggesting poor prognosis. It was detectable that B4GALNT1 expression interrelated with the infiltration extent of natural killer T cells in HCC tissues. B4GALNT1-fueled cell proliferation and enhanced resistance to apoptosis in HCC cells.  \nConclusion: B4GALNT1 is a strong regulator of HCC progression and holds promise as a marker for prognosis and a hallmark for therapy in HCC.  \nThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.  \n© 2025 The Author(s). Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.  \nJournal of Clinical Laboratory Analysis, 2025; 39:e25155 1 of 14  \n[https://doi.org/10.1002/jcla.25155](https://doi.org/10.1002/jcla.25155)  \n1 | Introduction  \nHepatocellular carcinoma (HCC), a typical primary liver cancer, appears as the 4th globally recognized commonly encountered cancer mortality cause [1, 2]. Mainstay ri","cbCaidC37FWHcf5A","https://ap.wps.com/l/cbCaidC37FWHcf5A","pdf",15080468,14,"English","# Introduction\n## Differential roles and risk factors in HCC\n## Rationale for B4GALNT1\n# Methods and datasets\n## Bioinformatic analyses\n## Immune infiltration and pathway analysis\n## Cell experiments\n# Results\n## Expression, methylation, and prognosis\n## Immune cell infiltration correlations\n## Effects on proliferation and apoptosis\n# Conclusion","[{\"question\":\"What is the main focus of this study on B4GALNT1 in hepatocellular carcinoma?\",\"answer\":\"The study investigates whether B4GALNT1 regulates HCC cell proliferation and apoptosis and how it relates to prognosis and immune-related features via the PI3K–AKT–mTOR pathway.\"},{\"question\":\"How were dataset analyses used to evaluate B4GALNT1 in HCC?\",\"answer\":\"Expression differences were compared across TCGA_LIHC and GEO datasets, while prognosis was assessed using Cox regression. Mutation links to CpG island methylation and outcomes were examined using cBioPortal and MethSurv.\"},{\"question\":\"What experimental approach was used to test the functional role of B4GALNT1?\",\"answer\":\"A lentiviral vector expressing B4GALNT1 was constructed, and B4GALNT1 was knocked down in HepG2 cells. Cell proliferation assays and flow cytometry were then used to measure proliferation and apoptosis effects.\"}]","B4GALNT1 Regulates Hepatocellular Carcinoma Cell Proliferation and Apoptosis via the PI3K-AKT-mTOR Pathway | PDF",1790158177,35]