[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-376639-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-376639-en":130},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","autophagy-related-microrna-tumor-mir-125b-and-thyroid-cancers","Autophagy-Related MicroRNA: Tumor miR-125b and Thyroid Cancers","","Autophagy operates as a cellular stress response that degrades components; dysfunction links it to multiple pathologies, including cancer. Thyroid cancer is the most prevalent malignant endocrine tumor, motivating a study using bioinformatics to examine autophagy-related genes and proteins and confirm findings in tissue from 103 thyroid patients. Real-time PCR measured hsa-miR-125b, while expression differences and BRAFV600E mutation status were analyzed to connect miR-125b regulation, autophagy mechanisms, tumor invasion, and recurrence risk.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/autophagy-related-microrna-tumor-mir-125b-and-thyroid-cancers/376639/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/autophagy-related-microrna-tumor-mir-125b-and-thyroid-cancers/376639.png","ImageObject",300,407,{"name":42,"@type":43},"Lucas Martin","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-24","2026-09-24",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",5,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What question does the study address about autophagy and thyroid cancer?","Question",{"text":63,"@type":64},"The study aims to explore the role of autophagy-related genes and proteins in thyroid cancers using bioinformatics analysis and tissue-sample detection, with emphasis on miR-125b regulation.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How was miR-125b measured in the patient samples?",{"text":68,"@type":64},"hsa-miR-125b levels were detected by real-time PCR in cancer samples from 103 patients with thyroid pathology.",{"name":70,"@type":61,"acceptedAnswer":71},"What findings connect miR-125b and BRAFV600E with tumor behavior?",{"text":72,"@type":64},"The study reports reduced miR-125b expression in papillary thyroid cancer versus benign tumors, and a further decrease associated with BRAFV600E mutations, linking miR-125b to invasion and recurrence risk.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},376639,1790504910,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,100,105,109,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":97,"show_sort_weight":98,"slug":99},"Comic",60,"comic",{"id":101,"doc_module":4,"doc_module_name":25,"category_name":102,"show_sort_weight":103,"slug":104},6,"Technology",50,"technology",{"id":106,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":107,"slug":108},7,40,"healthcare",{"id":110,"doc_module":4,"doc_module_name":25,"category_name":111,"show_sort_weight":112,"slug":113},8,"Research & Report",30,"research-report",{"id":115,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},9,"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":55,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":106,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":139,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":145},8796095360427,"https://ap-avatar.wpscdn.com/davatar_994ba38a5ba835b3df7d355c54d3ed8d","Article  \nAutophagy-Related MicroRNA: Tumor miR-125b and Thyroid Cancers  \nLiudmila V. Spirina 1,2, *, Irina V. Kovaleva 1,2, Svetlana Yu. Chizhevskaya 1,2, Anastasiya V. Chebodaeva 1 and Nataliya V. Tarasenko 1,3  \nCitation: Spirina, L.V.; Kovaleva, I.V.; Chizhevskaya, S.Y.; Chebodaeva, A.V.; Tarasenko, N.V.  \nAutophagy-Related MicroRNA: Tumor miR-125b and Thyroid Cancers. Genes 2023, 14, 685 . [https://doi.org/10.3390/](https://doi.org/10.3390/)[ ](https://doi.org/10.3390/)genes14030685  \nAcademic Editors: Hsiuying Wang and Y-h. Taguchi  \nReceived: 7 February 2023  \nRevised: 1 March 2023  \nAccepted: 7 March 2023  \nPublished: 9 March 2023  \nCopyright: © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://](https://)[ ](https://)[creativecommons.org/licenses/by/](creativecommons.org/licenses/by/)[ ](creativecommons.org/licenses/by/)[4.0/](4.0/)) .  \n1 Ministry of Health of Russia, Siberian State Medical University, 634050 Tomsk, Russia  \n2 Cancer Research Institute, Tomsk National Research Medical Center of the Russian Academy of Sciences, 634050 Tomsk, Russia  \n3 Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Sciences, 634050 Tomsk, Russia  \n* Correspondence: [spirinalvl@mail.ru](spirinalvl@mail.ru); Tel.: +7-9609758577  \nAbstract: Background: Autophagy is a stress response mechanism that causes cellular components to degrade. Its defects were associated with multiple pathologies, including cancers. Thyroid cancer is known to be the most prevalent form of malignant neoplasm among endocrine tumors. The aim of the study was to seek and comprehensively explore the role of autophagy related genes and proteins play in thyroid cancers through bioinformatics analysis with their detection in the tissue samples. Methods: Bioinformatics analysis was performed to investigate autophagy related proteins and genes involvement in thyroid cancer progression. The experimental verification was done in cancer samples of one hundred and three patients with thyroid pathology included in the study. The miR-125blevel was detected by PCR in real time. Results and discussion: The bioinformatics analysis verified themiR-125b as a regulatory mechanism in autophagy. Its expression in patients with PTC was reduced by 6.75 times in cancer patients compared to the patients with benign tumors. The BRAFV600E mutations were associated with a decrease in hsa-miR-125b expression by 12.67 times compared to tumors with the wild-type gene. Conclusions: Our findings revealed involvement of the autophagy related proteins in cancer progression. The significant mechanisms of regulation are non-coding RNA sequences implicated in a variety of oncogenic processes. We found that miR-125b is a potential maker in thyroid cancer invasion, BRAV600E mutational status and risk of recurrence.  \nKeywords: papillary thyroid cancer; autophagy; hsa-miR-125b; tumor invasion; BRAFV600E; risk of recurrence  \n1. Introduction  \nAutophagy is a stress response mechanism that leads to the degradation of cellular components, the defects of which are associated with multiple conditions [1] . Mounting evidence reveals that autophagy is a factor in thyroid cancer. Modulating autophagy has become a prominent target for improving the monitoring of cancer patients [2] . The principal triggers that determine the induction of autophagy are starvation, reactive oxygen species, endoplasmic stress and hypoxia. There are multiple channels that modulate autophagy, such as AMPK and PI3K orchestrating the protein cascade (ULK1, ATG16L1, MAP1LC3B, PRKAA1, ATG12, ATG14, ATG5, mTOR and BECN1) [2] .  \nPapillary thyroid cancer (PTC) is the most frequent differentiated tumor. The malignant neoplasms rate in the thyroid gland is increasing annually worldwide [3] . The molecular mechanisms underlying the progress of","cbCaipQgUMnLBdYJ","https://ap.wps.com/l/cbCaipQgUMnLBdYJ","pdf",3637737,18,"English","# Abstract\n# 1. Introduction\n## Autophagy and thyroid cancer context\n## Key molecular triggers and pathways\n## Non-coding RNAs and microRNA relevance\n## Autophagy pathway proteins and regulators","[{\"question\":\"What question does the study address about autophagy and thyroid cancer?\",\"answer\":\"The study aims to explore the role of autophagy-related genes and proteins in thyroid cancers using bioinformatics analysis and tissue-sample detection, with emphasis on miR-125b regulation.\"},{\"question\":\"How was miR-125b measured in the patient samples?\",\"answer\":\"hsa-miR-125b levels were detected by real-time PCR in cancer samples from 103 patients with thyroid pathology.\"},{\"question\":\"What findings connect miR-125b and BRAFV600E with tumor behavior?\",\"answer\":\"The study reports reduced miR-125b expression in papillary thyroid cancer versus benign tumors, and a further decrease associated with BRAFV600E mutations, linking miR-125b to invasion and recurrence risk.\"}]","Autophagy-Related MicroRNA: Tumor miR-125b and Thyroid Cancers | PDF",1790220400,45]