[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-297564-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-297564-en":130},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","atcc-medium-2324-preparation-tsa-with-glucose","ATCC Medium 2324 - Preparation - TSA with glucose","","ATCC medium 2324 recipe for preparing TSA supplemented with glucose. Includes the full component list and quantities per liter, including tryptic soy broth, glucose, agar (when needed), and distilled deionized water. Specifies pH adjustment to 7.0 and autoclaving at 121°C for 15 minutes. Provides instructions to add a filtered BSA solution at 50 mL/L and details how to prepare BSA solution from bovine serum albumin (fraction V) using filtration steps before sterilization.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/atcc-medium-2324-preparation-tsa-with-glucose/297564/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/atcc-medium-2324-preparation-tsa-with-glucose/297564.png","ImageObject",300,407,{"name":42,"@type":43},"Rhys","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-23","2026-09-18",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",8,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What are the main components and amounts for ATCC medium 2324 per 1.0 L?","Question",{"text":63,"@type":64},"Tryptic soy broth is listed as 3.0 g, glucose as 10.0 g, agar as 15.0 g if needed, and distilled deionized water to 1.0 L.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How should the medium pH and sterilization be handled?",{"text":68,"@type":64},"Adjust pH to 7.0, then autoclave at 121°C for 15 minutes.",{"name":70,"@type":61,"acceptedAnswer":71},"How do you prepare and add the BSA solution for ATCC medium 2324?",{"text":72,"@type":64},"Prepare BSA solution by dissolving 5 g bovine serum albumin (fraction V) in 50 mL distilled deionized water with vigorous stirring and gentle heating. Pre-filter through a large-pore filter, sterilize via a 0.2-micrometer membrane filter, then add filtered BSA solution at 50 mL/L.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},297564,1790068870,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,110,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":108,"slug":109},7,40,"healthcare",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":111,"show_sort_weight":112,"slug":113},"Research & Report",30,"research-report",{"id":115,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},9,"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":97,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":107,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":22,"language":139,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":12,"update_tm":143,"read_time":30},687207024643,"https://ap-avatar.wpscdn.com/davatar_3d24733baf745e90a7e4bdd5f77d97b2","ATCC medium: 2324 1/10 TSA with glucose  \nTryptic soy broth . . . . . . . . . . . . . . . . . .3 .0 g Glucose . . . . . . . . . . . . . . . . . . . . . . . . . . .10 .0 g Agar if needed . . . . . . . . . . . . . . . . . . . .15 .0 g Distilled deionized water   1 .0 L  \nAdjust pH to 7.0. Autoclave at 121C for 15 minutes . Add filtered BSA solution (50 ml/L) .  \nBSA Solution:  \nDissolve 5 g bovine serum albumin (fraction V) in 50 ml distilled deionized water with vigorous stirring and gentle heating . Pre-filter the solution through a large-pore filter before sterilizing through a 0 .2-micrometer membrane filter .","cbCaissuckBp9ikG","https://ap.wps.com/l/cbCaissuckBp9ikG","pdf",139524,"English","# Medium 2324 Composition\n## Base components and volumes\n## pH adjustment and autoclave conditions\n## Addition of glucose and BSA solution\n## BSA solution preparation and filtration","[{\"question\":\"What are the main components and amounts for ATCC medium 2324 per 1.0 L?\",\"answer\":\"Tryptic soy broth is listed as 3.0 g, glucose as 10.0 g, agar as 15.0 g if needed, and distilled deionized water to 1.0 L.\"},{\"question\":\"How should the medium pH and sterilization be handled?\",\"answer\":\"Adjust pH to 7.0, then autoclave at 121°C for 15 minutes.\"},{\"question\":\"How do you prepare and add the BSA solution for ATCC medium 2324?\",\"answer\":\"Prepare BSA solution by dissolving 5 g bovine serum albumin (fraction V) in 50 mL distilled deionized water with vigorous stirring and gentle heating. Pre-filter through a large-pore filter, sterilize via a 0.2-micrometer membrane filter, then add filtered BSA solution at 50 mL/L.\"}]","ATCC Medium 2324 - Preparation - TSA with glucose | PDF",1789745811]