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Methods: Analyze a subset of 71 7-year-old children from the ELLF cohort using in vitro TLR7/8 agonist stimulation ± anti-IgE, assessed by flow cytometry, cytokines, and pDC transcriptional profiling.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/asthma-and-lower-airway-disease-reduced-type-i-interferon-by-plasmacytoid-dendritic-cells-and-asthma-in-school-aged-children/440633/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/asthma-and-lower-airway-disease-reduced-type-i-interferon-by-plasmacytoid-dendritic-cells-and-asthma-in-school-aged-children/440633.png","ImageObject",300,407,{"name":42,"@type":43},"dawugda","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-11-03","2026-09-29",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",12,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"Why are plasmacytoid dendritic cells (pDC) important in childhood asthma?","Question",{"text":63,"@type":64},"pDC are rare immune cells that produce type I interferons (IFN-I), which are central to antiviral responses. The document links altered pDC function with asthma development and pathology in children.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How did the study assess pDC responses in children?",{"text":68,"@type":64},"The study used an in vitro assay with pDC stimulation by a TLR7/8 agonist, with and without anti-IgE. Responses were evaluated using flow cytometry, multiplex cytokine assays, and transcriptional analysis.",{"name":70,"@type":61,"acceptedAnswer":71},"What was the main finding about IFN-I versus TNF-only pDC responses?",{"text":72,"@type":64},"Individuals who showed an IFN-I response after stimulation had a lower proportion of asthma than those showing a TNF-only response. 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Jones1,2 | Alice A. White3 | Mari Takashima4 | Wen Ray Lee4 | Matthew D. Wong4 | Dwan Vilcins4 | Ulrich Kadolsky5 | Ali Sadiq Cheema5 | Alka Saxena5 | Anthony Bosco2,6,7 | Keith Grimwood8 | Peter D. Sly4 | Deborah H. Strickland1 | Jonatan Leffler1  \n1Wal-Yan Respiratory Research Centre, The Kids Research Institute Australia, University of Western Australia, Perth, Western Australia,  \nAustralia | 2INSiGENe Pty Ltd, Perth, Western Australia, Australia | 3The Kids Research Institute Australia, University of Western Australia, Perth, Western Australia, Australia | 4Child Health Research Centre, The University of Queensland, South Brisbane, Queensland, Australia | 5Genomics WA, Perth, Western Australia, Australia | 6Asthma and Airway Disease Research Center, University of Arizona, Tucson, Arizona, USA | 7Department of Immunobiology, University of Arizona College of Medicine, Tucson, Arizona, USA | 8School of Medicine and Dentistry, Griffith University, Gold Coast, Queensland, Australia  \nCorrespondence: Jonatan Leffler ([jonatan.leffler@thekids.org.au](jonatan.leffler@thekids.org.au))  \nReceived: 17 February 2025 | Revised: 11 June 2025 | Accepted: 29 June 2025  \nFunding: The study was funded by the Wal-yan Respiratory Research Centre, [and J.L. is](and J.L. is) supported by a fellowship from the Stan Perron Charitable Foundation.  \nKeywords: childhood asthma | IgE | interferon response | plasmacytoid dendritic cells  \nABSTRACT  \nBackground: Allergic sensitization and reduced ability to respond to viral infections may contribute to virus-induced wheeze and asthma development in young children. Plasmacytoid dendritic cells (pDC) are rare immune cells that produce type I interferons (IFN-I) and play a key role in orchestrating immune responses against viruses.  \nObjective: To further evaluate the function of pDC in children with asthma.  \nMethods: This study was based on a subset of 71 children from the Early Life Lung Function (ELLF) cohort at the age of 7years. As part ofthe ELLF study, participants were characterized for atopic sensitization, viral infection history, and lung function testing. pDC responses to a TLR7/8 agonist were assessed in the presence or absence of anti-IgE using an in vitro assay. Responses were evaluated utilizing flow cytometry, multiplexed cytokine assays, and transcriptional analysis of isolated pDC.  \nResults: pDC responses varied considerably across individuals, and those who responded with IFN-I following stimulation showed a lower proportion of asthma compared to those who responded with TNF-only. A TNF-only response was associated with increased atopy and reduced upregulation of IFN-associated genes. Anti-IgE stimulation reduced pDC activation, and the reduction was associated with baseline expression of the IgE receptor (FcεR1) . A reduction in a gene module centralized around genes such as TPM2, LILRA4, and CLEC4C was also observed.  \nConclusion: Together, these findings suggest that pDC responses are variable, associated with asthma, and appear influenced by environmental stimuli. This response thus appears to be an important aspect of asthma pathology in children.  \n1 | Introduction  \nIn young children, wheezing during a viral respiratory infection is common and affects almost 50% of children before  \nthe age of 5 years [1] . However, some children experience recurrent episodes of wheeze, and a subset of these are subsequently diagnosed with asthma. Impaired innate immune responses to viruses [2], atopic sensitization [3] and repeated  \nThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.  \n© 2025 Th","cbCaiirehtTFV7jt","https://ap.wps.com/l/cbCaiirehtTFV7jt","pdf",5180766,"English","# Abstract\n## Background\n## Objective\n## Methods\n## Results and Conclusion\n# Introduction","[{\"question\":\"Why are plasmacytoid dendritic cells (pDC) important in childhood asthma?\",\"answer\":\"pDC are rare immune cells that produce type I interferons (IFN-I), which are central to antiviral responses. The document links altered pDC function with asthma development and pathology in children.\"},{\"question\":\"How did the study assess pDC responses in children?\",\"answer\":\"The study used an in vitro assay with pDC stimulation by a TLR7/8 agonist, with and without anti-IgE. Responses were evaluated using flow cytometry, multiplex cytokine assays, and transcriptional analysis.\"},{\"question\":\"What was the main finding about IFN-I versus TNF-only pDC responses?\",\"answer\":\"Individuals who showed an IFN-I response after stimulation had a lower proportion of asthma than those showing a TNF-only response. TNF-only responses were associated with increased atopy and reduced upregulation of IFN-associated genes.\"}]","Asthma and Lower Airway Disease - Reduced Type-I Interferon by Plasmacytoid Dendritic Cells and Asthma in School-Aged Children | PDF",1790692794]