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This study examined how BRCA1, PPAP2B, and KCNN3 gene polymorphisms relate to sheep litter size and aimed to identify genetic markers for high fecundity. Whole-genome resequencing and Sequenom MassARRAY SNP genotyping assessed polymorphic loci across five breeds, comparing high- and low-prolificacy groups and testing Hardy–Weinberg equilibrium.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/association-analysis-of-brca1-ppap2b-and-kcnn3-gene-polymorphisms-with-litter-size-in-sheep/438039/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/association-analysis-of-brca1-ppap2b-and-kcnn3-gene-polymorphisms-with-litter-size-in-sheep/438039.png","ImageObject",300,407,{"name":92,"@type":93},"Mafia Boss","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-09-30","2026-09-29",true,{"@type":102,"interactionType":103,"userInteractionCount":14},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the main research goal of this study?","Question",{"text":112,"@type":113},"To assess the relationship between BRCA1, PPAP2B, and KCNN3 gene polymorphisms and sheep litter size, and to identify potential genetic markers for high fecundity.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"Which methods were used to analyze the gene polymorphisms?",{"text":117,"@type":113},"Whole-genome resequencing combined with Sequenom MassARRAY SNP genotyping was used to analyze polymorphic loci in the three genes across five sheep breeds.",{"name":119,"@type":110,"acceptedAnswer":120},"What associations were found with litter size?",{"text":121,"@type":113},"BRCA1 and KCNN3 polymorphisms showed significant breed-specific frequency differences, while only the PPAP2B g.77092130 G>A locus demonstrated a significant association with litter size in Small-Tailed Han sheep, with higher frequency of the GG genotype.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},438039,1790794887,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":14,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":34,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144},962090760730,"https://ap-avatar.wpscdn.com/davatar_9964176cb1d06d4a9deccf72a44ae3dc","Wiley  \nVeterinary Medicine International Volume 2025, Article ID 2771616, 7 pages [https://doi.org/10.1155/vmi/2771616](https://doi.org/10.1155/vmi/2771616)  \nResearch Article  \nAssociation Analysis of BRCA1, PPAP2B, and KCNN3 Gene Polymorphisms With Litter Size in Sheep  \nYu He, 1,2 Xiangyu Wang, 1 Chen Liang ,2 and Mingxing Chu 1  \n1 State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China  \n2 College of Animal Science, Shanxi Agricultural University, Taigu, Shanxi, 030801, China  \nCorrespondence should be addressed to Chen Liang; cekiv@163.com and Mingxing Chu; [mxchu@263.net](mxchu@263.net)  \nReceived 21 April 2025; Revised 26 September 2025; Accepted 31 October 2025  \nAcademic Editor: Juan Pablo Dami´an  \nCopyright © 2025 Yu He et al. Veterinary Medicine International published by John Wiley & Sons Ltd. Tis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.  \nBackground: Reproductive efciency is a key economic trait in sheep production, with litter size being a major determinant of productivity. Genetic polymorphisms in candidate genes such as BRCA1, PPAP2B, and KCNN3 may infuence fecundity, but their association with sheep prolifcacy remains to be fully elucidated. Identifying genetic markers linked to high litter size could enhance breeding strategies for improved reproductive performance.  \nObjective: Tis study aimed to (1) investigate the relationship between BRCA1, PPAP2B, and KCNN3 gene polymorphisms and sheep litter size and (2) identify potential genetic markers for high fecundity in sheep.  \nMethods: Whole-genome resequencing combined with Sequenom MassARRAY SNP genotyping was used to analyze fve polymorphic loci in BRCA1, PPAP2B, and KCNN3 across fve sheep breeds, including high-prolifcacy breeds (Small-Tailed Han sheep, Hu Sheep, and Cele Black Sheep) and low-prolifcacy breeds (Sunite sheep and Bamei mutton sheep) . Small-Tailed Han sheep were selected for association analysis with litter size. Chi-square tests assessed Hardy–Weinberg equilibrium (HWE), and association studies evaluated genotype efects on reproductive traits.  \nResults: BRCA1 gene polymorphisms (g.42581575 T > C, g.42560830 A > T, g.42548851 C > T) exhibited signifcant diferences (p \u003C 0.01) in genotype and allele frequencies between high-and low-prolifcacy breeds. All three loci were in HWE (p > 0.05) except in Hu sheep. Te KCNN3 g.103522805 C >A locus also showed signifcant frequency diferences (p \u003C 0.01) between breeds but deviated from HWE in Bamei mutton sheep (p \u003C 0.05).Only the PPAP2B g.77092130 G>A locus demonstrated a signifcant association (p > 0.05) with litter size in Small-Tailed Han sheep, where the GG genotype was more frequent than the AA genotype in the second parity.  \nConclusion: Tis study identifed BRCA1, PPAP2B, and KCNN3 as potential genetic markers infuencing sheep litter size. Te BRCA1 and KCNN3 polymorphisms showed strong breed-specifc associations, while the PPAP2B GG genotype may favor higher fecundity in Small-Tailed Han sheep. Tese fndings provide valuable insights for marker-assisted selection in sheep breeding programs aimed at improving reproductive efciency. Further functional studies are needed to validate the biological mechanisms underlying these genetic associations.  \nKeywords: BRCA1; KCNN3; litter size; PPAP2B; sheep; SNPs  \n1. Introduction  \nReproductive performance stands as one of the most economically signifcant traits in modern sheep production systems [1], with fecundity parameters including estrous  \ncyclicity, breeding seasonality, and ovulation rate serving as critical determinants of fock proftability [2] . Te inherent variability in these physiological traits, particularly in ovulation rate, creates substantial diferences in production efciency across breeds and indivi","cbCaijn9pw0uhXvG","https://ap.wps.com/l/cbCaijn9pw0uhXvG","pdf",705972,"English","# Background\n# Objective\n# Methods\n# Results\n# Conclusion\n# Keywords\n# Introduction","[{\"question\":\"What is the main research goal of this study?\",\"answer\":\"To assess the relationship between BRCA1, PPAP2B, and KCNN3 gene polymorphisms and sheep litter size, and to identify potential genetic markers for high fecundity.\"},{\"question\":\"Which methods were used to analyze the gene polymorphisms?\",\"answer\":\"Whole-genome resequencing combined with Sequenom MassARRAY SNP genotyping was used to analyze polymorphic loci in the three genes across five sheep breeds.\"},{\"question\":\"What associations were found with litter size?\",\"answer\":\"BRCA1 and KCNN3 polymorphisms showed significant breed-specific frequency differences, while only the PPAP2B g.77092130 G\\u003eA locus demonstrated a significant association with litter size in Small-Tailed Han sheep, with higher frequency of the GG genotype.\"}]","Association Analysis of BRCA1, PPAP2B, and KCNN3 Gene Polymorphisms With Litter Size in Sheep | PDF",1790683974,18]