[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-331977-105":59,"doc-detail-331977-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","assessment-of-the-cytotoxic-effect-of-carboxymethyl-chitosan-loaded-amygdalin-nanoparticles-against-human-normal-and-cancer-cell-lines","Assessment of the Cytotoxic Effect of Carboxymethyl Chitosan-Loaded Amygdalin Nanoparticles Against Human Normal and Cancer Cell Lines","","Development of antitumor drugs increasingly focuses on natural products, and amygdalin is highlighted as a cyanoglycoside with anticarcinogenic potential upon hydrogen cyanide release. This study synthesizes and evaluates carboxymethyl chitosan (CMC) nanoparticles as delivery vehicles for encapsulated amygdalin to cancer and normal cell lines. CMC-Am NPs are characterized by particle size, zeta potential, chemical structure, and dielectric properties, and invitro drug release and cytotoxicity are assessed using MTT assays. Results show ~129 nm particles, zeta potential around −43 mV, sustained release up to 24 h, and enhanced anticancer activity on HCT-116 and MCF-7 with safety on BJ1 normal cells.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/assessment-of-the-cytotoxic-effect-of-carboxymethyl-chitosan-loaded-amygdalin-nanoparticles-against-human-normal-and-cancer-cell-lines/331977/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/assessment-of-the-cytotoxic-effect-of-carboxymethyl-chitosan-loaded-amygdalin-nanoparticles-against-human-normal-and-cancer-cell-lines/331977.png","ImageObject",300,407,{"name":92,"@type":93},"Olivia Brown","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-29","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the main objective of the study?","Question",{"text":112,"@type":113},"To synthesize and evaluate carboxymethyl chitosan nanoparticles as drug-delivery agents for encapsulating amygdalin and delivering it to cancer and normal cell lines.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were the CMC-amygdalin nanoparticles characterized and what release behavior was observed?",{"text":117,"@type":113},"They were characterized by particle size, surface charge (zeta potential), chemical structure, and dielectric properties; invitro release showed sustained amygdalin release over 24 hours.",{"name":119,"@type":110,"acceptedAnswer":120},"What do the cytotoxicity results show compared with free amygdalin?",{"text":121,"@type":113},"Compared with free amygdalin, amygdalin-loaded CMC nanoparticles showed significant anticancer effects on HCT-116 and MCF-7 cells while remaining safe for normal BJ1 cells.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},331977,1790515781,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},16904993612988,"https://ap-avatar.wpscdn.com/davatar_a8503ba1806abce46bf441b54a3ca4cd","Research  \nAssessment of the cytotoxic effect of carboxymethyl chitosan‑loaded amygdalin nanoparticles against human normal and cancer cell lines  \nA. S. El‑Houssiny1 · A. A. F. Soliman2 · K. N. Abdel‑Nour1  \nReceived: 26 January 2024 / Accepted: 17 March 2024  \n© The Author(s) 2024 OPEN  \nAbstract  \nIn recent years, the development of antitumor drugs has been dedicated to natural products. Amygdalin is a natural herbal cyanoglycoside that has anticarcinogenic effect on many types of cancers once hydrogen cyanide (HCN) is released. The main objective of the present study is to synthesize and investigate the potential of carboxymethyl chitosan nanoparticles (CMC NPs) as drug delivery agents for amygdalin encapsulation and its delivery to cancer and normal cell lines. In this study, carboxymethyl chitosan nanoparticles encapsulated with amygdalin (CMC-Am NPs) were prepared and characterized through their particle size, surface charge, chemical structure and dielectric properties. Also, the invitro drug release of amygdalin from CMC NPs was studied. Additionally, the cytotoxcity of the amygdalin and CMC-loaded amygdalin NPs were evaluated through MTT assay. The results showed that the prepared CMC-loaded amygdalin NPs exhibited a small particle size of 129 nm, high zeta potential value of − 43 mV and confirmed the amygdalin stability and compatibility with CMC NPs. Furthermore, the CMC NPs demonstrated sustained release of amygdalin during 24 h. Moreover, compared to free amygdalin, amygdalin-loaded CMC NPs have significant anti-cancerous effect on human colon HCT-116 and breast MCF-7 cancer cell lines while being safe on normal cells BJ1 . In conclusion, CMC NPs can be employed as an efficient drug delivery vehicle for controlled and sustained amygdalin release with enhanced cytotoxicityon malignant cells without harming normal cells.  \nKeywords Carboxymethyl chitosan · Malignant cells · Amygdalin · Nanoparticles · Anticancer  \n1 Introduction  \nAmygdalin, often known as vitamin B17, is an aromatic cyanogenic glycoside that is found in almond, peach, and apricot seeds [1]. It consists of one molecule of benzaldehyde, one molecule of hydrocyanic acid and two molecules of glucose. Amygdalin has been widely utilized as a natural anticancer medication because of the presence of cyanogenic group in its structure, which makes it an effective alternative for traditional cancer treatments [2] . The cancer cell contains enzyme beta-glucosidases which release the benzaldehyde and hydrogen cyanide (HCN) from amygdalin, which is a toxic compound. The cyanide ion decomposing carcinogenic cells by interfering with oxygen utilized by these cells and blocking the nutrient source of tumor cells [3] .  \nMany experiments supported that the amygdalin has potential cytotoxic and anti-tumor effects and can be considered as a potential alternative for anticancer drugs [4–7]. Makarević et al. [8, 9] found that amygdalin reduced tumor cell proliferation in bladder and prostate cancer cell lines resulting in a significant delay in cell cycle progression. Ibrahim  \n* A. S. El-Houssiny, [asmaa_sayedelhoussiny@yahoo.com |](asmaa_sayedelhoussiny@yahoo.com |1Microwave)[1](asmaa_sayedelhoussiny@yahoo.com |1Microwave)[Microwave](asmaa_sayedelhoussiny@yahoo.com |1Microwave) Physics and Dielectrics Department, National Research Centre, Dokki, Cairo, Egypt. 2Pharmacognosy Department, National Research Centre, Dokki, Cairo, Egypt.  \nDiscover Nano  \n(2024) 19:62  \n| [https://doi.org/10.1186/s11671-024-03998-7](https://doi.org/10.1186/s11671-024-03998-7)  \net al. [10] found that the apricot juice has an anticancer effect against colon cancer by reducing colon cancer biomarkers TNF-α, NF-κB, IL-1β and IL6 levels in the serum. Arshi et al. [11] observed that the amygdalin decreased growth and proliferation of stomach cancer (AGS), lung cancer (A549) and breast cancer (MCF7) through down-regulating the expression level of two antiapoptotic genes.  \nVarious targeted thera","cbCainmQ8ryfd93u","https://ap.wps.com/l/cbCainmQ8ryfd93u","pdf",3203131,13,"English","# Abstract\n# Keywords\n# 1 Introduction\n## Background on amygdalin\n## Rationale for nanoformulation and CMC\n# 2 Materials and methods\n## 2.1 Materials\n## 2.2 Preparation of CMC-Amygdalin NPs","[{\"question\":\"What is the main objective of the study?\",\"answer\":\"To synthesize and evaluate carboxymethyl chitosan nanoparticles as drug-delivery agents for encapsulating amygdalin and delivering it to cancer and normal cell lines.\"},{\"question\":\"How were the CMC-amygdalin nanoparticles characterized and what release behavior was observed?\",\"answer\":\"They were characterized by particle size, surface charge (zeta potential), chemical structure, and dielectric properties; invitro release showed sustained amygdalin release over 24 hours.\"},{\"question\":\"What do the cytotoxicity results show compared with free amygdalin?\",\"answer\":\"Compared with free amygdalin, amygdalin-loaded CMC nanoparticles showed significant anticancer effects on HCT-116 and MCF-7 cells while remaining safe for normal BJ1 cells.\"}]","Assessment of the Cytotoxic Effect of Carboxymethyl Chitosan-Loaded Amygdalin Nanoparticles Against Human Normal and Cancer Cell Lines | PDF",1789996105,33]