[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-detail-441851-en":3,"detail-sidebar-cat-0-en-105":31,"doc-seo-441851-105":80},{"code":4,"msg":5,"data":6},0,"success",{"doc_id":7,"user_id":8,"nickname":9,"user_avatar":10,"doc_module":4,"category_id":11,"category_name":12,"doc_title":13,"doc_description":14,"doc_content":15,"file_id":16,"file_url":17,"file_type":18,"file_size":19,"view_count":20,"is_deleted":4,"is_public":21,"is_downloadable":21,"audit_status":21,"page_count":22,"language":23,"language_code":24,"site_id":25,"html_lang":24,"table_of_contents":26,"faqs":27,"seo_title":28,"seo_description":14,"update_tm":29,"read_time":30},441851,2336475401981,"Chumphorn","https://ap-avatar.wpscdn.com/avatar/22000c94efd8d5204d?x-image-process=image/resize,m_fixed,w_180,h_180&k=1786935347598174694",8,"Research & Report","Assessment of Neolignan Profiles during the Growth Period of Magnolia fargesii Leaves and Their MUC5AC Inhibition Potential","The flower buds of Magnolia fargesii (Xinyi) have traditionally been used to treat inflammatory respiratory diseases, including chronic obstructive pulmonary disease (COPD). Prior work identified seven lignans that reduce airway inflammation by suppressing epidermal growth factor receptor (EGFR) activation, but leaf composition across growth stages remains underexplored. Neolignan-nebulous content in leaves collected May–July was linked to anti-inflammatory activity and mucin 5AC (MUC5AC) expression inhibition.","This article is licensed under CC-BY-NC-ND 4.0   \n[http://pubs.acs.org/journal/acsodf](http://pubs.acs.org/journal/acsodf)  Article   \nAssessment of Neolignan Profiles during the Growth Period of Magnolia fargesii Leaves and Their MUC5AC Inhibition Potential  \nSun Ho Kam,⊥ Su-Yeon Lee,⊥ Hyoung-Geun Kim,⊥ Seul Beom Yu, Doo-Young Kim, Seon Min Oh, Hyun-Jae Jang, Jongmin Ahn, Eun Sol Oh, Su Ui Lee, Taehoon Oh, Sung-Kyun Ko, Bang Yeon Hwang,* Sei-Ryang Oh, * and Hyung Won Ryu *  \n Cite This: ACS Omega 2025, 10, 59230−59241  \nRead Online  \n\n|  |  |  |  |  |  |\n| --- | --- | --- | --- | --- | --- |\n| ACCESS   | Metrics & More |  |  Article Recommendations |  | *sı Supporting Information |\n\nABSTRACT: The flower buds of Magnolia fargesii (Xinyi) have traditionally been used to treat inflammatory respiratory diseases, including chronic obstructive pulmonary disease (COPD). While previous studies identified seven lignans in Magnolia that alleviate airway inflammation by inhibiting epidermal growth factor receptor (EGFR) activation, the chemical composition and biological activity of the leaves especially at different growth stages remain understudied. In this study, the relationship between neolignan content, anti-inflammatory activity, and mucin 5AC (MUC5AC) expression inhibition in M. fargesii leaves collected from May to July was investigted. Optimal extraction conditions were established using 80% ethanol (EtOH) at 40 °C for 2 h. Metabolite  \nprofiling was performed using ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS), followed by multivariate statistical analyses including principal component analysis (PCA), orthogonal partial least-squares-discriminant analysis (OPLS-DA), and S-plots. Eight neolignan compounds were identified and structurally confirmed through nuclear magnetic resonance (NMR), circular dichroism (CD) spectroscopy, and mass spectrometry (MS). Quantitative analysis revealed notable variations in metabolite content and bioactivity depending on the collection time. Notably, compounds 2, 5, and 6 increased by 1.3−1.4 fold in July compared with June (mg/g, DW), consistent with the enhanced anti-inflammatory and MUC5AC inhibitory effects observed during this period. In contrast, compounds 4, 7, and 8 peaked in June and then declined.  \n1. INTRODUCTION  \nMagnolia fargesii (Magnoliaceae), traditionally known as“Xinyi”, has been extensively utilized in East Asian medicine for treating respiratory ailments such as allergic rhinitis, sinusitis, and chronic obstructive pulmonary disease (COPD).1 Previous studies have identified several lignans from the flower buds of M. fargesii, including magnolin, epimagnolin, fargesin, and aschantin, which exhibit notable anti-inflammatory activities by inhibiting pathways like EGFR, ERK1/2, and PI3K/Akt.2 These lignans are widely recognized for their broad range of therapeutic activities, such as antioxidant, anti-inflammatory, anticancer, and hormonemodulating effects.3−7 Accordingly, flower buds have been regarded as the principal therapeutic organ of M. fargesii. However, other organs of M. fargesii, particularly the leaves, remain underexplored despite their distinct phytochemical profiles. The chemical composition of leaves, which varies across growth stages, may confer unique pharmacological properties that differ from those of the flower buds. Since phytochemical diversity strongly influences therapeutic efficacy, elucidating these organ-specific and stage-specific  \nmetabolomic variations is essential for defining their respective pharmacological roles and optimizing their use in medicine.  \nPlant secondary metabolite biosynthesis is influenced by both abiotic and biotic factors, leading to dynamic changes in compound abundance. 8−12 Among major pathways, the phenylpropanoid pathway generates lignans and flavonoids that are central to both plant defense and human therapeutic applications. 13−15 These biosynthetic dyna","cbCaipFOxw4sFaLX","https://ap.wps.com/l/cbCaipFOxw4sFaLX","pdf",5312111,2,1,12,"English","en",105,"# Abstract\n## Background and rationale\n## Methods and metabolite profiling\n## Key findings across growth stages\n## Biological interpretation and implications","[{\"question\":\"What was investigated in Magnolia fargesii leaves?\",\"answer\":\"Neolignan content in leaves collected from May to July and how it relates to anti-inflammatory activity and MUC5AC expression inhibition.\"},{\"question\":\"How were optimal extraction and metabolite profiling performed?\",\"answer\":\"Extraction used 80% ethanol at 40 °C for 2 h, and metabolite profiling used UPLC coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) with multivariate analyses.\"},{\"question\":\"Which growth stage changes were most notable for neolignan compounds?\",\"answer\":\"Compounds 2, 5, and 6 increased 1.3–1.4 fold in July versus June, matching stronger anti-inflammatory and MUC5AC inhibitory effects, while compounds 4, 7, and 8 peaked in June then declined.\"}]","Assessment of Neolignan Profiles during the Growth Period of Magnolia fargesii Leaves and Their MUC5AC Inhibition Potential | PDF",1790697833,30,{"code":4,"msg":5,"data":32},[33,38,42,47,52,57,62,64,69,72,76],{"id":21,"doc_module":4,"doc_module_name":34,"category_name":35,"show_sort_weight":36,"slug":37},"Document","Story & 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was investigated in Magnolia fargesii leaves?","Question",{"text":128,"@type":129},"Neolignan content in leaves collected from May to July and how it relates to anti-inflammatory activity and MUC5AC expression inhibition.","Answer",{"name":131,"@type":126,"acceptedAnswer":132},"How were optimal extraction and metabolite profiling performed?",{"text":133,"@type":129},"Extraction used 80% ethanol at 40 °C for 2 h, and metabolite profiling used UPLC coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) with multivariate analyses.",{"name":135,"@type":126,"acceptedAnswer":136},"Which growth stage changes were most notable for neolignan compounds?",{"text":137,"@type":129},"Compounds 2, 5, and 6 increased 1.3–1.4 fold in July versus June, matching stronger anti-inflammatory and MUC5AC inhibitory effects, while compounds 4, 7, and 8 peaked in June then declined.","https://schema.org",{"og:url":100,"og:type":140,"og:title":13,"og:site_name":111,"og:description":14},"article",{"robots":142,"canonical":100},"index,follow",{"doc_id":7,"site_id":25},1790766246]