[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-427519-105":59,"doc-detail-427519-en":134},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":127,"head_meta":129,"extra_data":131,"updated_unix":133},105,"en","assessment-of-male-reproductive-aging-implications-of-sertoli-cells-senescence-abstract-citation-id-igaf1222877","ASSESSMENT OF MALE REPRODUCTIVE AGING - IMPLICATIONS OF SERTOLI CELL’S SENESCENCE - Abstract citation ID igaf122.2877","","Cancer survival has increased, creating a growing population of early life cancer survivors, yet the long-term reproductive aging effects of chemotherapy and radiotherapy remain underexplored. This study assessed whether doxorubicin and gamma irradiation induce senescence in Sertoli cells and how this impacts male reproductive aging. Sertoli cells were exposed to doxorubicin and irradiation; senescence markers (CDKN2A, CDKN1A, GDF15, IL-6, IL-8) were validated 10 days later. DIA proteomics on Orbitrap identified 6,304 proteins, with therapy-specific pathway shifts consistent with disrupted Sertoli paracrine function and reduced sexual reproduction.",{"@graph":69,"@context":126},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/assessment-of-male-reproductive-aging-implications-of-sertoli-cells-senescence-abstract-citation-id-igaf1222877/427519/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/assessment-of-male-reproductive-aging-implications-of-sertoli-cells-senescence-abstract-citation-id-igaf1222877/427519.png","ImageObject",300,407,{"name":92,"@type":93},"River Wang","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-10","2026-09-29",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118,122],{"name":109,"@type":110,"acceptedAnswer":111},"What was the purpose of exposing Sertoli cells to doxorubicin and gamma irradiation?","Question",{"text":112,"@type":113},"To evaluate the senescence-inducing potential of doxorubicin and irradiation in Sertoli cells and determine the implications for male reproductive aging.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was Sertoli cell senescence confirmed in the study?",{"text":117,"@type":113},"Senescence was validated 10 days after exposure by upregulating senescence markers including CDKN2A, CDKN1A, GDF15, IL-6, and IL-8.",{"name":119,"@type":110,"acceptedAnswer":120},"What did the proteomic analysis reveal about protein regulation after treatments?",{"text":121,"@type":113},"Proteomics identified 6,304 proteins; doxorubicin downregulated 1,509 and upregulated 1,443 proteins, while irradiation downregulated 1,090 and upregulated 1,125 proteins.",{"name":123,"@type":110,"acceptedAnswer":124},"What biological processes and pathways were enriched, and what does that imply?",{"text":125,"@type":113},"Downregulated proteins were enriched for microtubule-based processes, cell division, and sexual reproduction, while upregulated proteins were enriched for transmembrane/cell transport and immune response pathways, consistent with dysregulated Sertoli cell paracrine functions.","https://schema.org",{"og:url":83,"og:type":128,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":130,"canonical":83},"index,follow",{"doc_id":132,"site_id":62},427519,1790766246,{"code":4,"msg":5,"data":135},{"doc_id":132,"user_id":136,"nickname":92,"user_avatar":137,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":138,"file_id":139,"file_url":140,"file_type":141,"file_size":142,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":14,"language":143,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":144,"faqs":145,"seo_title":146,"seo_description":67,"update_tm":147,"read_time":24},1099514067438,"https://ap-avatar.wpscdn.com/avatar/100002539ee87300030?x-image-process=image/resize,m_fixed,w_180,h_180&k=1780474512215547542","Innovation in Aging, 2025, Vol. 9, No. S2 849  \nAbstract citation ID: igaf122.2877  \nASSESSMENT OF MALE REPRODUCTIVE AGING IMPLICATIONS OF SERTOLI CELL’S SENESCENCE  \nElikanah Olusayo Adegoke1Alexandra Montesinos1,  \nReema Banarjee2, Amit Dey2, and Nathan Basisty2, 1. National Institute on Aging, Baltimore, Maryland, United States,  \n2. National Institute on Aging, Bethesda, Maryland,  \nUnited States  \nAdvances in cancer detection and improved treatment strategies have markedly increased survival rate for cancers (pediatric), leading to a growing population of early life cancer survivors. However, long term reproductive effects of chemotherapy (doxorubicin) and radiotherapy (irradiation) on reproductive aging has received less attention. Sertoli cells support, nourish, and protect spermatogenic cells in the testis. To evaluate the senescence inducing potential of doxorubicin and irradiation on Sertoli cells and the consequent implications in male reproductive aging, Sertoli cells in culture were exposed to doxorubicin and gamma radiation, and senescence was validated ten days after exposure by confirming the upregulation of senescence markers: CDKN2A, CDKN1A, GDF15, IL-6, IL-8 . DIA proteomic analysis was conducted on the Orbitrap mass spectrometer. Data analysis was performed on Spectronaut and R. The proteomic analysis identified 6,304 proteins. Among these, 1,509 proteins were downregulated and 1,443 were upregulated in doxorubicin treatment. After irradiation, 1,090 proteins were downregulated, while 1,125 proteins were upregulated. Microtubule-based process, cell division, and sexual reproduction biological processes were enriched among downregulated proteins following both treatments, suggesting loss of key functions supporting reproduction and phagocytosis associated with Sertoli cells. Furthermore, transmembrane/cell transport pathways and immune response, were enriched among upregulated proteins in both treatments, known senescence associated pathways that are consistent with dysregulation of normal Sertoli cell paracrine functions. 45% of protein changes were overlapping between  \n850 Innovation in Aging, 2025, Vol. 9, No. S2  \nboth treatments. In conclusion, we demonstrate that both doxorubicin and irradiation can induce Sertoli cells to senescence and consequently result in reproductive aging marked by decreased sexual reproduction.","cbCaisZrPw7QrlLy","https://ap.wps.com/l/cbCaisZrPw7QrlLy","pdf",1496232,"English","# Abstract\n## Study aim and rationale\n## Experimental design and senescence validation\n## Proteomics workflow and results\n## Enriched pathways and interpretation\n## Conclusion","[{\"question\":\"What was the purpose of exposing Sertoli cells to doxorubicin and gamma irradiation?\",\"answer\":\"To evaluate the senescence-inducing potential of doxorubicin and irradiation in Sertoli cells and determine the implications for male reproductive aging.\"},{\"question\":\"How was Sertoli cell senescence confirmed in the study?\",\"answer\":\"Senescence was validated 10 days after exposure by upregulating senescence markers including CDKN2A, CDKN1A, GDF15, IL-6, and IL-8.\"},{\"question\":\"What did the proteomic analysis reveal about protein regulation after treatments?\",\"answer\":\"Proteomics identified 6,304 proteins; doxorubicin downregulated 1,509 and upregulated 1,443 proteins, while irradiation downregulated 1,090 and upregulated 1,125 proteins.\"},{\"question\":\"What biological processes and pathways were enriched, and what does that imply?\",\"answer\":\"Downregulated proteins were enriched for microtubule-based processes, cell division, and sexual reproduction, while upregulated proteins were enriched for transmembrane/cell transport and immune response pathways, consistent with dysregulated Sertoli cell paracrine functions.\"}]","ASSESSMENT OF MALE REPRODUCTIVE AGING - IMPLICATIONS OF SERTOLI CELL’S SENESCENCE - Abstract citation ID igaf122.2877 | PDF",1790644072]