[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-439249-105":3,"detail-sidebar-cat-0-en-105":80,"doc-detail-439249-en":130},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":73,"head_meta":75,"extra_data":77,"updated_unix":79},105,"en","assessing-the-role-of-exogenous-no-on-plants-and-microbial-communities-in-soil","Assessing the role of exogenous NO on plants and microbial communities in soil","","Nitric oxide (NO) is a reactive signaling gas affecting plant growth, stress responses, and microbial functions. In soil, NO arises from microbial activity, plant metabolism, and physicochemical processes, yet exogenous NO impacts on plants and root-associated microbiota remain insufficiently characterized. The study tested NO exposure effects on plant physiology, trace gas fluxes, N cycling, and root microbiota abundance, diversity, and composition using Arabidopsis and tomato mesocosms with NO flushing.",{"@graph":14,"@context":72},[15,34,55],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & Report",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/assessing-the-role-of-exogenous-no-on-plants-and-microbial-communities-in-soil/439249/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/assessing-the-role-of-exogenous-no-on-plants-and-microbial-communities-in-soil/439249.png","ImageObject",300,407,{"name":42,"@type":43},"Damian","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-09-30","2026-09-29",true,{"@type":52,"interactionType":53,"userInteractionCount":26},"InteractionCounter",{"@type":54},"ViewAction",{"@type":56,"mainEntity":57},"FAQPage",[58,64,68],{"name":59,"@type":60,"acceptedAnswer":61},"What question does the study address about exogenous NO?","Question",{"text":62,"@type":63},"How NO exposure influences plant physiology and root-associated microbial communities in soil, including related trace gas fluxes and nitrogen cycling.","Answer",{"name":65,"@type":60,"acceptedAnswer":66},"How was NO exposure delivered in the experiments?",{"text":67,"@type":63},"Two 37-day mesocosm experiments used NO flushing for 3–4 day periods at either 0 ppbv or 400 ppbv while monitoring CO2, N2O, and NO fluxes.",{"name":69,"@type":60,"acceptedAnswer":70},"What main outcome was observed for Arabidopsis compared with tomato?",{"text":71,"@type":63},"NO at 400 ppbv increased leaf area in Arabidopsis and modulated plant growth-defense balance gene expression, while no effect on tomato plants or associated microbiota was detected.","https://schema.org",{"og:url":32,"og:type":74,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":76,"canonical":32},"index,follow",{"doc_id":78,"site_id":7},439249,1790742620,{"code":4,"msg":81,"data":82},"success",[83,87,91,95,100,105,110,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":84,"show_sort_weight":85,"slug":86},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":88,"show_sort_weight":89,"slug":90},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":92,"show_sort_weight":93,"slug":94},"Exam",70,"exam",{"id":96,"doc_module":4,"doc_module_name":25,"category_name":97,"show_sort_weight":98,"slug":99},5,"Comic",60,"comic",{"id":101,"doc_module":4,"doc_module_name":25,"category_name":102,"show_sort_weight":103,"slug":104},6,"Technology",50,"technology",{"id":106,"doc_module":4,"doc_module_name":25,"category_name":107,"show_sort_weight":108,"slug":109},7,"Healthcare",40,"healthcare",{"id":111,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":112,"slug":113},8,30,"research-report",{"id":115,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},9,"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":96,"slug":129},19,"General","general",{"code":4,"msg":81,"data":131},{"doc_id":78,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":111,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":26,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":139,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":145},137451208677,"https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d","ISME Communications, 2025, 5(1), ycaf237  \n[https://doi.org/10.1093/ismeco/ycaf237](https://doi.org/10.1093/ismeco/ycaf237)  \nAdvance access publication: 16 December 2025  \nOriginal Article  \nAssessing the role of exogenous NO on plants and microbial communities in soil  \nEduardo Pérez-Valera 1 ,‡, Logapragasan Subramaniam 2 ,‡, Pauline Trapet 1 , Antoine Berger 1 , Marie-Christine Breuil1 , Florian Engelsberger2 , Nicolas Brüggemann 3 , Klaus Butterbach-Bahl 2 ,4 , Michael Dannenmann 2 , David Wendehenne 1 , Laurent Philippot 1 , *  \n1Univ Bourgogne Europe, INRAE, Institut Agro Dijon, Agroécologie, 17 Rue Sully, Dijon 21000, France  \n2 Division “Terrestrial Bio-Geo-Chemistry”, Karlsruhe Institute of Technology (KIT), Institute for Meteorology and Climate Research Atmospheric Environmental Research (IMK-IFU), Kreuzeckbahnstr. 19, Garmisch-Partenkirchen 82467, Germany  \n3Agrosphere (IBG-3), Forschungszentrum Jülich GmbH, Institute of Bio-and Geosciences, Jülich 52428, Germany  \n4 Center for Landscape Research in Sustainable Agricultural Futures-Land-CRAFT, Department of Agroecology, Aarhus University, Ole Worms Allé 3, Aarhus C 8000, Denmark  \n*Corresponding author. Univ Bourgogne Europe, INRAE, Institut Agro Dijon, Agroécologie, 17 Rue Sully, Dijon 21000, France. E-mail: [Laurent.philippot@inrae.fr](Laurent.philippot@inrae.fr)[ ](Laurent.philippot@inrae.fr)‡Eduardo Pérez-Valera and Logapragasan Subramaniam contributed equally to this work.  \nAbstract  \nNitric oxide (NO) is a reactive gas that functions as a signaling molecule regulating plant growth and stress responses, while also exerting various roles for microorganisms. In soil, NO is produced through microbial activity, plant metabolism, and physico-chemical processes. However, the impact of exogenous NO on plant physiology and the associated root microbiota remains unexplored. Here, we evaluated the effects of NO exposure on plant physiology, trace gas fluxes and N cycling, as well as the abundance, diversity, and composition of root-associated microbiota. We conducted two 37-day experiments with either Arabidopsis thaliana or tomato (Solanum lycopersicum) plants using innovative plant–soil mesocosms that allowed NO flushing while monitoring the CO2, N 2O and NO fluxes. The mesocosms were subjected to four NO flushing periods (3–4 days each) at 0 ppbv or 400 ppbv. Our results revealed that exogenous NO400 exerted plant-specific effects. While flushing with NO400 had no effect on tomato plants or associated microbiota, it increased leaf area in Arabidopsis and modulated the expression of two genes involved in plant growth-defense balance compared to flushing with NO0. These changes in Arabidopsis physiology were concomitant with modest alterations in the fungal community and a decrease in the abundance of bacterial ammonia-oxidizers, 15N recovery as NO3 − , and cumulative CO2 fluxes. However, it is still unclear how much of these effects were indirectly driven by plant–soil feedbacks. Our findings offer intriguing insights into the possible, though modest, effects of exogenous NO in shaping plant–microbe interactions.  \nKeywords: nitric oxide; rhizosphere; Arabidopsis thaliana; Solanum lycopersicum; N2O; CO 2; nitrogen cycling; bacteria; fungi  \nIntroduction  \nNitric oxide (NO) is a reactive trace gas which plays a critical role in tropospheric chemistry by affecting ozone and aerosol formation, as well as acid deposition. It originates from fossil fuel combustion, biomass burning, lightning, emissions from soilsand other biogenic activity [1–3] . In the soil atmosphere, NOis primarily produced through both physico-chemical reactions and microbial processes [4 , 5], although it can also be generated by plants [6] . Microorganisms involved in the nitrification and denitrification processes are the main biogenic contributors to NO production [7] . Previous studies showed that NO emitted from soil originates primarily from the uppermost layer of the soil profile, with NO con","cbCaioUJv3UKJBf0","https://ap.wps.com/l/cbCaioUJv3UKJBf0","pdf",2421346,15,"English","# Abstract\n# Introduction\n## Background on NO in atmosphere and soil\n## Biological roles of NO in microorganisms\n## Study rationale and context","[{\"question\":\"What question does the study address about exogenous NO?\",\"answer\":\"How NO exposure influences plant physiology and root-associated microbial communities in soil, including related trace gas fluxes and nitrogen cycling.\"},{\"question\":\"How was NO exposure delivered in the experiments?\",\"answer\":\"Two 37-day mesocosm experiments used NO flushing for 3–4 day periods at either 0 ppbv or 400 ppbv while monitoring CO2, N2O, and NO fluxes.\"},{\"question\":\"What main outcome was observed for Arabidopsis compared with tomato?\",\"answer\":\"NO at 400 ppbv increased leaf area in Arabidopsis and modulated plant growth-defense balance gene expression, while no effect on tomato plants or associated microbiota was detected.\"}]","Assessing the role of exogenous NO on plants and microbial communities in soil | PDF",1790687953,38]