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Chou–Talalay analysis and HPLC quantify synergistic effects with docetaxel and bioactive molecules, while assessing selectivity toward normal human fibroblasts.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & 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fractions analyzed?",{"text":72,"@type":64},"High-performance liquid chromatography (HPLC) was used for quantitative determination of bioactive molecules present in both phenolic and terpene fractions.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},331420,1790418168,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,111,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & 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cytotoxicity of phenolic and terpene fractions extracted from Iraqi Prunus arabica on AMJ13 and SK-GT-4 human cancer cell lines [version 1; peer review: 1 approved with reservations]  \nMatin Adil Mahmood 1,2, Abdulkareem Hameed Abd2, EnasJawad Kadhim3  \n1 Department of Pharmacology, College of Pharmacy, Al-Kitab University, Altun Kopre, Kirkuk, Iraq  \n2 Department of Pharmacology, College of Medicine, Al-Nahrain University, Kadhimiya, Baghdad, Iraq  \n3 Department of Pharmacognosy and Medicinal Plants, College of Pharmacy, University of Baghdad, Baghdad, Iraq  \nv1  \nFirst published: 21 Apr 2023, 12:433  \n[https://doi.org/10.12688/f1000research.131336.1](https://doi.org/10.12688/f1000research.131336.1)  \nSecond version: 24 Jul 2023, 12:433  \n[https://doi.org/10.12688/f1000research.131336.2](https://doi.org/10.12688/f1000research.131336.2)  \n[Latest published:](Latest published: 10 Sep 2024)[ 10 Sep 2024](Latest published: 10 Sep 2024), 12:433  \n[https://doi.org/10.12688/f1000research.131336.3](https://doi.org/10.12688/f1000research.131336.3)  \nAbstract  \nBackground: Breast and esophageal cancer are the most aggressive and prominent causes of death worldwide. In addition, these cancers showed resistance to current chemotherapy regimens with limited success rates and fatal outcomes. Recently many studies reported the significant cytotoxic effects of phenolic and terpene fractions extracted from various Prunus species against different cancer cell lines. As a result, it has a good chance to be tested as a complement or replacement for standard chemotherapies.  \nMethods: The study aimed to evaluate the cytotoxicity of phenolic and terpene fractions extracted from Iraqi Prunus arabica on breast (AMJ13) and esophageal (SK-GT-4) cancer cell lines by using the MTTassay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide). Analysis using the Chou-Talalay method was performed to assess the synergistic effect between the extracted fractions and chemotherapeutic agent (docetaxel) . Moreover, high-performance liquid chromatography (HPLC) analysis was conducted for the quantitative determination of different bioactive molecules of both phenolic and terpene fractions in the extract.  \nResults: According to the findings, the treatment modalities significantly decreased cancer cell viability of AMJ13 and SK-GT-4 and had insignificant cytotoxicity on the normal cells (normal human fibroblast cell line) (all less than 50% cytotoxicity) . Analysis with ChouTalalay showed a strong synergism with docetaxel on both cancer cell lines (higher cytotoxicity even in low concentrations) and failed to induce cytotoxicity on the normal cells. Important flavonoid glycosidesand terpenoids were detected by HPLC, in particularly, ferulic acid,  \nOpen Peer Review  \n\n| Approval Status  |  |  |\n| --- | --- | --- |\n|  | 1 | 2 |\n| version 3 |  |  |\n| (revision) |  |  |\n| 10 Sep 2024 |  |  |\n| version 2\u003Cbr>(revision) | \u003Cbr>view | \u003Cbr>view |\n| 24 Jul 2023 | |  |\n| version 1 | |  |\n| 21 Apr 2023 | view |  |\n\n1. Serap Sahin-Bolukbasi , Afyonkarahisar Health Sciences University, Afyonkarahisar, Turkey  \n2. Heba A. S. El-Nashar , Afyonkarahisar Health Sciences University, Afyonkarahisar, Turkey  \nAny reports and responses or comments on the  \narticle can be found at the end of the article.  \ncatechin, chlorogenic acid, β-sitosterol, and campesterol. Conclusions: In conclusion, the extracted fractions selectively inhibited the proliferation of both cancer cell lines and showed minimal cytotoxicity on normal cells. These fractions could be naturally derived drugs for treating breast and esophageal cancers.  \nKeywords  \nPrunus arabica, Phenolic, Terpene, Cytotoxicity assay, HPLC, ChouTalalay  \nThis article is included in the Cell & Molecular  \nBiology gateway.  \nCorresponding author: Matin Adil Mahmood ([matin.a.mahmood@uoalkitab.edu.iq](matin.a.mahmood@uoalkitab.edu.iq))  \nAuthor roles: Mahmood MA: Conceptualization, Data Curatio","cbCaivies4knuPXz","https://ap.wps.com/l/cbCaivies4knuPXz","pdf",4324198,"English","# Abstract\n## Methods\n## Results\n## Conclusions\n# Introduction","[{\"question\":\"What is the main purpose of assessing cytotoxicity in this study?\",\"answer\":\"To evaluate how phenolic and terpene fractions from Iraqi Prunus arabica affect viability of AMJ13 and SK-GT-4 cancer cell lines and determine selectivity versus normal cells.\"},{\"question\":\"Which experimental methods were used to measure cytotoxicity and interactions?\",\"answer\":\"Cytotoxicity was measured using the MTT assay, and synergistic effects between the fractions and docetaxel were assessed using the Chou–Talalay method.\"},{\"question\":\"How was the chemical composition of the fractions analyzed?\",\"answer\":\"High-performance liquid chromatography (HPLC) was used for quantitative determination of bioactive molecules present in both phenolic and terpene fractions.\"}]","Assessing the cytotoxicity of phenolic and terpene fractions extracted from Iraqi Prunus arabica on AMJ13 and SK-GT-4 human cancer cell lines | PDF",1789993205]