[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-501593-105":59,"doc-detail-501593-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","applied-biosystems-stepone-and-steponeplus-real-time-pcr-systems-reagent-guide","Applied Biosystems StepOne and StepOnePlus Real-Time PCR Systems Reagent Guide","","Reagent Guide for the Applied Biosystems StepOne and StepOnePlus Real-Time PCR systems, providing licensing notices and trademark information plus structured guidance for using TaqMan and SYBR Green reagent categories. Includes chapter overviews for preparing experiments, selecting experiment, reagent, and assay types, and practical workflows for quantitation and genotyping studies. Covers design guidelines for quantitation experiments (1-step vs 2-step RT-PCR, singleplex vs multiplex) and genotyping assay selection and customization.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/applied-biosystems-stepone-and-steponeplus-real-time-pcr-systems-reagent-guide/501593/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/applied-biosystems-stepone-and-steponeplus-real-time-pcr-systems-reagent-guide/501593.png","ImageObject",300,407,{"name":92,"@type":93},"Guten Appetit_Joghurt","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-23","2026-10-03",true,{"@type":102,"interactionType":103,"userInteractionCount":19},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the purpose of this Reagent Guide for the StepOne and StepOnePlus systems?","Question",{"text":112,"@type":113},"It organizes information needed to select and use reagents for supported real-time PCR workflows, including guidance tied to experiment and assay choices.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"Which reagent types are covered in the guide?",{"text":117,"@type":113},"The guide includes sections for TaqMan reagents and SYBR Green reagents, along with guidance on selecting the appropriate reagent type.",{"name":119,"@type":110,"acceptedAnswer":120},"What topics are included for quantitation experiments?",{"text":121,"@type":113},"Quantitation coverage includes how to choose quantitation methods, decide between 1-step or 2-step RT-PCR, select singleplex or multiplex PCR, and follow design guidelines for assays and reagent setup.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},501593,1791421107,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":19,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},1374408768420,"https://ap-avatar.wpscdn.com/davatar_6f874abed73319feea01a86fa6f0fab8","Applied Biosystems StepOne ™ and StepOnePlus ™  \nReal-Time PCR Systems  \nApplied Biosystems StepOne ™ and StepOnePlus ™ Real-Time PCR  \nSystems  \nReagent Guide  \n© Copyright 2008, 2010 Applied Biosystems. All rights reserved.  \nInformation in this document is subject to change without notice. Applied Biosystems assumes no responsibility for any errors that may appear in this document.  \nAPPLIED BIOSYSTEMS DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. IN NO EVENT SHALL APPLIED BIOSYSTEMS BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE THEREOF.  \nFor Research Use Only. Not for use in diagnostic procedures.  \nNOTICE TO PURCHASER: Label License  \nThe StepOne™ and StepOnePlus™ Real-Time PCR Systems are covered by US patents and corresponding claims in their non-US counterparts, owned by Applied Biosystems Inc. No right is conveyed expressly, by implication, or by estoppel under any other patent claim, such as claims to apparatus, reagents, kits, or methods such as 5′ nuclease methods. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.  \nNOTICE TO PURCHASER:  \nPLEASE REFER TO THE USER'S GUIDE OR PRODUCT INSERT OF THE REAGENTS NAMED HEREIN FOR LIMITED LABEL LICENSE OR DISCLAIMER INFORMATION.  \nTRADEMARKS:  \nApplied Biosystems, AB (Design), MicroAmp, Primer Express, and VIC are registered trademarks, and FAM, JOE, MicroAmp, MultiScribe, NED, ROX, StepOne, StepOnePlus, TAMRA, TET, and VeriFlex are trademarks of Applied Biosystems or its subsidiaries in the U.S. and/or certain other countries.  \nAmpErase, AmpliTaq Gold, and TaqMan are registered trademarks of Roche Molecular Systems, Inc.  \nSYBR is a registered trademark of Molecular Probes, Inc.  \nAll other trademarks are the sole property of their respective owners.  \nPart Number 4379704 Rev. E 06/2010  \nContents  \nPreface  \nHow to Use This Guide .................................................. vii  \nHow to Obtain More Information ............................................ ix  \nHow to Obtain Support .................................................. xii  \nChapter 1 Introduction About the StepOne™ and StepOnePlus™ Systems ............................ 1-2  \nSupported Consumables ............................................... 1-4  \nPreparing for Experiments ............................................... 1-6  \nSelect the Experiment Type .............................................. 1-7  \nSelect the Reagent Type ................................................ 1-8  \nSelect the Assay Type .................................................. 1-9  \nChapter 2 Reagent Overview  \nOverview ............................................................ 2-2  \nTaqMan® Reagents .................................................... 2-2  \nSYBR® Green Reagents ................................................ 2-4  \nSelecting the Appropriate Reagent Type .................................... 2-6  \nMinimizing DNA Contaminants ........................................... 2-7  \nChapter 3 Quantitation Experiments  \nSection 3.1: About Quantitation Experiments .............................. 3-3  \nOverview ............................................................ 3-4  \nSelect a Quantitation Method ............................................ 3-5  \nSelect 1-Step or 2-Step RT-PCR .......................................... 3-8  \nSelect Singleplex or Multiplex PCR ....................................... 3-10  \nSelect the Reagent Type ............................................... 3-12  \nSelect the Assay Type ................................................. ","cbCaih2EtqO3xsXc","https://ap.wps.com/l/cbCaih2EtqO3xsXc","pdf",2208912,148,"English","# Preface\n## How to Use This Guide\n## How to Obtain More Information\n## How to Obtain Support\n# Chapter 1 Introduction About the StepOne and StepOnePlus Systems\n## Supported Consumables\n## Preparing for Experiments\n## Select the Experiment Type\n## Select the Reagent Type\n## Select the Assay Type\n# Chapter 2 Reagent Overview\n## Overview\n## TaqMan Reagents\n## SYBR Green Reagents\n## Selecting the Appropriate Reagent Type\n## Minimizing DNA Contaminants\n# Chapter 3 Quantitation Experiments\n## About Quantitation Experiments\n## Design Guidelines\n# Chapter 4 Genotyping Experiments\n## About Genotyping Experiments\n## Design Guidelines","[{\"question\":\"What is the purpose of this Reagent Guide for the StepOne and StepOnePlus systems?\",\"answer\":\"It organizes information needed to select and use reagents for supported real-time PCR workflows, including guidance tied to experiment and assay choices.\"},{\"question\":\"Which reagent types are covered in the guide?\",\"answer\":\"The guide includes sections for TaqMan reagents and SYBR Green reagents, along with guidance on selecting the appropriate reagent type.\"},{\"question\":\"What topics are included for quantitation experiments?\",\"answer\":\"Quantitation coverage includes how to choose quantitation methods, decide between 1-step or 2-step RT-PCR, select singleplex or multiplex PCR, and follow design guidelines for assays and reagent setup.\"}]","Applied Biosystems StepOne and StepOnePlus Real-Time PCR Systems Reagent Guide | PDF",1791021092,373]