[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-354354-105":59,"doc-detail-354354-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","apoptotic-signaling-across-breast-cancer-subtypes-and-cryoablation-induced-tissue-injury","Apoptotic Signaling Across Breast Cancer Subtypes and Cryoablation-Induced Tissue Injury","","Apoptosis maintains tissue homeostasis, and its dysregulation drives breast cancer progression and therapeutic resistance. An integrative analysis was performed across five molecular breast cancer subtypes, and systemic apoptotic responses were compared following cryoablation of benign fibroadenomas. Gene expression profiling used mRNA microarrays validated by qRT-PCR and ELISA, with pathway activity assessed via MSigDB HALLMARK_APOPTOSIS. Results showed reduced intrinsic signaling, decreased ABI, pro-apoptotic downregulation, and stress-adaptive upregulation that intensified with tumor aggressiveness, alongside miRNA–mRNA interaction candidates, whereas cryoablation produced transient modulation followed by normalization.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/apoptotic-signaling-across-breast-cancer-subtypes-and-cryoablation-induced-tissue-injury/354354/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/apoptotic-signaling-across-breast-cancer-subtypes-and-cryoablation-induced-tissue-injury/354354.png","ImageObject",300,407,{"name":92,"@type":93},"Franzy","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-27","2026-09-22",true,{"@type":102,"interactionType":103,"userInteractionCount":29},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"How was apoptosis-related signaling analyzed across breast cancer subtypes?","Question",{"text":112,"@type":113},"Apoptosis signaling was assessed by integrative analysis of apoptosis-related signaling in breast cancer tissues using mRNA microarrays, validated by qRT-PCR and ELISA, and pathway activity scoring with MSigDB HALLMARK_APOPTOSIS.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What trends were observed as tumor aggressiveness increased?",{"text":117,"@type":113},"A progressive shift occurred toward reduced pro-apoptotic and enhanced stress-adaptive signaling, including decreased intrinsic pathway activity and ABI, pro-apoptotic gene downregulation, and stress-associated or cytoprotective gene upregulation, strongest in triple-negative and non-luminal HER2-positive cancers.",{"name":119,"@type":110,"acceptedAnswer":120},"How did cryoablation of benign fibroadenomas affect apoptotic responses?",{"text":121,"@type":113},"Fibroadenoma cryoablation was associated with transient systemic modulation of apoptosis-related genes and proteins, followed by gradual normalization, contrasting with malignant tissue dysregulation.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},354354,1790744037,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":29,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":56,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144},2336478945519,"https://ap-avatar.wpscdn.com/davatar_085a072bc5b1113ac321206ff7593b45","Article  \nApoptotic Signaling Across Breast Cancer Subtypes and Cryoablation-Induced Tissue Injury  \nAgata Panfil 1,*, Kacper Boro ´n 2,3,4,5, Tomasz Sirek 1,2,6, Agata Sirek 1, Nikola Zmarzły 1, Michalina Wróbel 1,7, Zbigniew Wróbel 1,7, Dariusz Boro ´n 1,4, Piotr Ossowski 1, Martyna Stefaniak 1, Paweł Ordon 1, Grzegorz Wyrobiec 8, Wojciech Kulej 1, Marcin Opławski 9,10, Bogusław Opławski 9,10, Natalia Lekston 1 and Beniamin Oskar Grabarek 1  \nAcademic Editor: Amelia Casamassimi  \nReceived: 21 April 2026  \nRevised: 4 June 2026  \nAccepted: 5 June 2026  \nPublished: 7 June 2026  \nCopyright: © 2026 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.  \n1 Collegium Medicum, WSB University, 41-300 Da˛browa Górnicza, Poland; [drtstierka@gmail.com](drtstierka@gmail.com) (T.S.); [agatasirek06@gmail.com](agatasirek06@gmail.com) (A.S.); [nikola.zmarzly@wsb.edu.pl](nikola.zmarzly@wsb.edu.pl) (N.Z.); [wrobelmichalina@o2.pl](wrobelmichalina@o2.pl) (M.W.); [zbi.wrobel@gmail.com](zbi.wrobel@gmail.com) (Z.W.); [dariusz@boron.pl](dariusz@boron.pl) (D.B.); [drpiotrossowski15@gmail.com](drpiotrossowski15@gmail.com) (P.O.); [martynastefaniakk@gmail.com](martynastefaniakk@gmail.com) (M.S.); [pawelordon4@gmail.com](pawelordon4@gmail.com) (P.O.); [wojciechkulej87@gmail.com](wojciechkulej87@gmail.com) (W.K.); [lekstonnatalia@gmail.com](lekstonnatalia@gmail.com) (N.L.); [bgrabarek7@gmail.com](bgrabarek7@gmail.com) (B.O.G.)  \n2 Department of Plastic Surgery, Faculty of Medicine, Academia of Silesia, 40-555 Katowice, Poland; [q375@gmail.com](q375@gmail.com)  \n3 Faculty of Medicine and Health Sciences, Andrzej Frycz Modrzewski Krakow University, 30-705 Kraków, Poland  \n4 Department of Gynecology and Obstetrics, TOMMED Specjalisci od Zdrowia, 40-662 Katowice, Poland  \n5 Department of Gynecology and Obstetrics with Gynecologic Oncology, Ludwik Rydygier Memorial Specialized Hospital, 31-826 Kraków, Poland  \n6 Department of Plastic and Reconstructive Surgery, Hospital for Minimally Invasive and Reconstructive Surgery in Bielsko-Biała, 43-316 Bielsko-Biala, Poland  \n7 Medi-Lab SC, Wróbel i Wspólnicy, 58-100 ´Swidnica, Poland  \n8 Department of Histology and Cell Pathology in Zabrze, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 41-808 Zabrze, Poland; [gwyrobiec@sum.edu.pl](gwyrobiec@sum.edu.pl)  \n9 Department of Gynecology and Obstetrics, Faculty of Medicine and Health Sciences, Andrzej Frycz Modrzewski University Kraków, 30-705 Kraków, Poland; [marcin.oplawski@gmail.com](marcin.oplawski@gmail.com) (M.O.); [oplawski.b@gmail.com](oplawski.b@gmail.com) (B.O.)  \n10 Faculty of Medicine, VIZJA University, 01-043 Warszawa, Poland  \n* [Correspondence: agatapanfil875@gmail.com](Correspondence: agatapanfil875@gmail.com)  \nAbstract  \nApoptosis maintains tissue homeostasis, and its dysregulation is closely associated with breast cancer progression and therapeutic resistance. We performed an integrative analysis of apoptosis-related signaling in breast cancer tissues across five molecular subtypes and compared these patterns with systemic apoptotic responses following cryoablation of benign fibroadenomas. Gene expression profiling was conducted using mRNA microarraysand validated by qRT-PCR and ELISA. Apoptosis pathway activity was assessed with the MSigDB HALLMARK_APOPTOSIS gene set, including intrinsic and extrinsic pathway scoring and an apoptotic balance index (ABI) . MicroRNA profiling combined within silico analyses identified potential miRNA–mRNA interactions. A progressive shift toward reduced pro-apoptotic and enhanced stress-adaptive signaling was observed with increasing tumor aggressiveness, most pronounced in triple-negative and non-luminal HER2-positive cancers. This pattern included reduced intrinsic pathway activity, decreased ABI, downregulation of pro-apoptotic genes (BIK, BMF, TXNIP), and upregulati","cbCaijP1L7NUIJiF","https://ap.wps.com/l/cbCaijP1L7NUIJiF","pdf",644091,"English","# Introduction\n## Apoptosis in tissue homeostasis and cancer\n## Intrinsic and extrinsic apoptotic pathways\n## Breast cancer molecular heterogeneity and subtype-specific behavior","[{\"question\":\"How was apoptosis-related signaling analyzed across breast cancer subtypes?\",\"answer\":\"Apoptosis signaling was assessed by integrative analysis of apoptosis-related signaling in breast cancer tissues using mRNA microarrays, validated by qRT-PCR and ELISA, and pathway activity scoring with MSigDB HALLMARK_APOPTOSIS.\"},{\"question\":\"What trends were observed as tumor aggressiveness increased?\",\"answer\":\"A progressive shift occurred toward reduced pro-apoptotic and enhanced stress-adaptive signaling, including decreased intrinsic pathway activity and ABI, pro-apoptotic gene downregulation, and stress-associated or cytoprotective gene upregulation, strongest in triple-negative and non-luminal HER2-positive cancers.\"},{\"question\":\"How did cryoablation of benign fibroadenomas affect apoptotic responses?\",\"answer\":\"Fibroadenoma cryoablation was associated with transient systemic modulation of apoptosis-related genes and proteins, followed by gradual normalization, contrasting with malignant tissue dysregulation.\"}]","Apoptotic Signaling Across Breast Cancer Subtypes and Cryoablation-Induced Tissue Injury | PDF",1790110245,48]