[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-detail-339939-en":3,"detail-sidebar-cat-0-en-105":31,"doc-seo-339939-105":80},{"code":4,"msg":5,"data":6},0,"success",{"doc_id":7,"user_id":8,"nickname":9,"user_avatar":10,"doc_module":4,"category_id":11,"category_name":12,"doc_title":13,"doc_description":14,"doc_content":15,"file_id":16,"file_url":17,"file_type":18,"file_size":19,"view_count":20,"is_deleted":4,"is_public":21,"is_downloadable":21,"audit_status":21,"page_count":22,"language":23,"language_code":24,"site_id":25,"html_lang":24,"table_of_contents":26,"faqs":27,"seo_title":28,"seo_description":14,"update_tm":29,"read_time":30},339939,7971461740886,"Theodore","https://ap-avatar.wpscdn.com/davatar_3d24733baf745e90a7e4bdd5f77d97b2",7,"Healthcare","Apoptosis induction and inhibition of invasion and migration in gastric cancer cells by Isoorientin studied using network pharmacology","Investigation of Isoorientin’s effects on apoptosis, proliferation, invasion, and migration in human gastric cancer HGC27 cells uses network pharmacology to predict drug and disease targets. Cell proliferation is quantified by CCK-8, while flow cytometry assesses apoptosis and cell-cycle distribution. Scratch and transwell assays evaluate invasion and migration, and qPCR plus western blot examine apoptosis-related gene and protein changes. Isoorientin suppresses proliferation, migration, and invasion, promotes apoptosis via Bax and caspase-3 upregulation, and reduces p-PI3K, p-AKT, and Bcl-2.","Song et al.  \nBMC Complementary Medicine and Therapies (2024) 24:309  \n[https://doi.org/10.1186/s12906-024-04605-z](https://doi.org/10.1186/s12906-024-04605-z)  \nBMC Complementary Medicine and Therapies  \n RESEARCH Open Access  \nApoptosis induction and inhibition of invasion and migration in gastric cancer cells by Isoorientin studied using network pharmacology  \nDan Song1,2, Maosheng Chen1, Xiangjun Chen1, Jiaojiao Xu1, Siqi Wu1, Yaxin Lyu 1 and Qin Zhao1,2*  \nAbstract  \nBackground To investigate the effects of Isoorientin on the apoptosis, proliferation, invasion, and migration of human gastric cancer cells (HGC27 cells) .  \nMethods We used network pharmacology to predict the targets of drugs and diseases. The CCK-8 assay was used to determine the effects of Isoorientin on the proliferation of HGC27 cells. Flow cytometry was employed to analyze the effects of Isoorientin on cell apoptosis and cell cycle distribution of HGC27 cells. Scratch test and transwell chamber test were conducted to assess the effects of Isoorientin on invasion and migration, respectively. Additionally, qPCR and western blot were performed to examine the impact of Isoorientin on apoptosis-related genes and protein expression, respectively.  \nResults The Isoorientin significantly inhibited the proliferation, migration, and invasion of HGC27 cells compared to the control group. Furthermore, Isoorientin induced apoptosis in HGC27 cells by upregulating the relative expression of Bax and caspase-3 while downregulating the relative expression of p-PI3K, p-AKT, and Bcl-2 proteins. Conclusion The Isoorientin exhibits inhibitory effects on the proliferation, invasion, and migration of HGC27 cells, and induces apoptosis in gastric cancer cells.  \nKeywords Gastric cancer, Isoorientin, Network pharmacology, Apoptosis, Invasion and migration  \n*Correspondence: Qin Zhao [qzhao@xzmu.edu.cn](qzhao@xzmu.edu.cn)  \n1 Joint Laboratory for Research on Active Components and Pharmacological Mechanism of Tibetan Materia Medica of Tibetan Medical Research Center of Tibet, School of Medicine, Xizang Minzu University, Xianyang 712082, China  \n2 Engineering Research Center of Tibetan Medicine Detection Technology, Ministry of Education, Xizang Minzu University, Xianyang 712082, China  \nIntroduction  \nGastric cancer (GC) is a common gastrointestinal malignancy worldwide. There were about 397,000 new cases of GC in China in 2016 and approximately 289,000 deaths. According to the National Central Cancer Registry of China, GC mortality is the third highest among all malignant tumors. Early cancer has no evident symptoms, and the early detection rate is low in China, with most patients being diagnosed in the advanced stage [1, 2]. Environmental and genetic factors can affect GC development; these include ethnicity, gender, age, smoking, diets high in nitrates and nitrites, infection with Helicobacter pylori and Epstein-Barr virus, previous stomach  \n© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. 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