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This study evaluates Casiopeínas® copper coordination compounds CasIIIia and CasIIgly for anti-migratory, anti-invasive, anti-tumoral, and antimetastatic effects in vitro and in vivo. The compounds induce apoptosis via reactive oxygen species and DNA damage and reduce TNBC viability dose-dependently, outperforming Cisplatin. CasIIIia and CasIIgly also suppress migration and inhibit tumor growth and metastatic foci in a syngeneic 4T1 model.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/antitumoral-and-antimetastatic-activity-by-mixed-chelate-copperii-compounds-casiopeinas-on-triple-negative-breast-cancer-in-vitro-and-in-vivo-models/339970/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/antitumoral-and-antimetastatic-activity-by-mixed-chelate-copperii-compounds-casiopeinas-on-triple-negative-breast-cancer-in-vitro-and-in-vivo-models/339970.png","ImageObject",300,407,{"name":92,"@type":93},"Theodore","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-29","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":44},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What research question does the study address about TNBC?","Question",{"text":112,"@type":113},"The study evaluates whether mixed chelate copper(II) compounds Casiopeínas® (CasIIIia and CasIIgly) can produce antitumoral and antimetastatic effects against triple-negative breast cancer in vitro and in vivo.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How do Casiopeínas® compounds induce cancer cell death according to the study?",{"text":117,"@type":113},"They induce apoptosis by generating reactive oxygen species (ROS) and causing DNA damage, as described in the abstract.",{"name":119,"@type":110,"acceptedAnswer":120},"How do Casiopeínas® compare with Cisplatin in TNBC experiments?",{"text":121,"@type":113},"Casiopeínas® reduce TNBC cell viability dose-dependently and inhibit migration at subtoxic concentrations; in vivo, CasIIIia and Cisplatin reduce tumor growth and metastatic foci, with Casiopeínas® reported as more efficient in cell viability assays.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},339970,1790038979,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":44,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":56,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144},7971461740886,"https://ap-avatar.wpscdn.com/davatar_3d24733baf745e90a7e4bdd5f77d97b2","Article  \nAntitumoral and Antimetastatic Activity by Mixed Chelate Copper(II) Compounds (Casiopeínas® ) on Triple-Negative Breast Cancer, In Vitro and In Vivo Models  \nMauricio M. González-Ballesteros 1, Luis Sánchez-Sánchez 2, Adrián Espinoza-Guillén 1,  \nJesús Espinal-Enríquez 3, Carmen Mejía 4, Enrique Hernández-Lemus 3 and Lena Ruiz-Azuara 1, *  \nCitation: González-Ballesteros, M.M.; Sánchez-Sánchez, L.;  \nEspinoza-Guillén, A.;  \nEspinal-Enríquez, J.; Mejía, C.;  \nHernández-Lemus, E.; Ruiz-Azuara, L. Antitumoral and Antimetastatic Activity by Mixed Chelate Copper(II) Compounds (Casiopeínas®) on Triple-Negative Breast Cancer, In Vitro and In Vivo Models. Int. J. Mol. Sci. 2024, 25, 8803. [https://doi.org/](https://doi.org/)[ ](https://doi.org/)[10.3390/ijms25168803](10.3390/ijms25168803)  \nAcademic Editors: Ignacio Camacho-Arroyo, Jorge Melendez-Zajgla, Mauricio Rodriguez-Dorantes and Mahara A. Valverde Ramírez  \nReceived: 4 July 2024  \nRevised: 1 August 2024  \nAccepted: 3 August 2024  \nPublished: 13 August 2024  \nCopyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://](https://)[ ](https://)[creativecommons.org/licenses/by/](creativecommons.org/licenses/by/)[ ](creativecommons.org/licenses/by/)[4.0/](4.0/)) .  \n1 Departamento de Química Inorgánica y Nuclear, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México 04510, Mexico; [mauricio.gb.117@outlook.com](mauricio.gb.117@outlook.com) (M.M.G.-B.)  \n2 Laboratorio de Biología Molecular del Cáncer, UMIEZ, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, Ciudad de México 09230, Mexico  \n3 Departamento de Genómica Computacional, Instituto Nacional de Medicina Genómica, Ciudad de México 14610, Mexico  \n4 Laboratorio de Biomedicina Interdisciplinaria, Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Ciudad de México 76230, Mexico  \n* Correspondence: [lenar701@gmail.com](lenar701@gmail.com)  \nAbstract: Triple-negative breast cancer (TNBC), accounting for 15–20% of all breast cancers, has one of the poorest prognoses and survival rates. Metastasis, a critical process in cancer progression, causes most cancer-related deaths, underscoring the need for alternative therapeutic approaches. This study explores the anti-migratory, anti-invasive, anti-tumoral, and antimetastatic effects of copper coordination compounds Casiopeína IIIia (CasIIIia) and Casiopeína IIgly (CasIIgly) on MDAMB-231 and 4T1 breast carcinoma cell lines in vitro and in vivo. These emerging anticancer agents, mixed chelate copper(II) compounds, induce apoptosis by generating reactive oxygen species (ROS) and causing DNA damage. Whole-transcriptome analysis via gene expression arrays indicated that subtoxic concentrations of CasIIIia upregulate genes involved in metal response mechanisms. Casiopeínas® reduced TNBC cell viability dose-dependently and more efficiently than Cisplatin. At subtoxic concentrations (IC 20), they inhibited random and chemotactic migration of MDA-MB-231 and 4T1 cells by 50–60%, similar to Cisplatin, as confirmed by transcriptome analysis. In vivo, CasIIIia and Cisplatin significantly reduced tumor growth, volume, and weight in a syngeneic breast cancer model with 4T1 cells. Furthermore, both compounds significantly decreased metastatic foci in treated mice compared to controls. Thus, CasIIIia and CasIIgly are promising chemotherapeutic candidates against TNBC.  \nKeywords: triple-negative breast cancer; metastasis; metallodrugs; copper; casiopeína  \n1. Introduction  \nCancer is a term that defines a set of multifactorial diseases, where malignant cells possess various phenotypic abnormalities, such as loss of differentiation, a high proliferation rate, and malignant tumors that tend to grow invasively, destroying surrounding as well as distant normal tissues and orga","cbCaipy32BrgOijY","https://ap.wps.com/l/cbCaipy32BrgOijY","pdf",5016875,"English","# Abstract\n# Introduction","[{\"question\":\"What research question does the study address about TNBC?\",\"answer\":\"The study evaluates whether mixed chelate copper(II) compounds Casiopeínas® (CasIIIia and CasIIgly) can produce antitumoral and antimetastatic effects against triple-negative breast cancer in vitro and in vivo.\"},{\"question\":\"How do Casiopeínas® compounds induce cancer cell death according to the study?\",\"answer\":\"They induce apoptosis by generating reactive oxygen species (ROS) and causing DNA damage, as described in the abstract.\"},{\"question\":\"How do Casiopeínas® compare with Cisplatin in TNBC experiments?\",\"answer\":\"Casiopeínas® reduce TNBC cell viability dose-dependently and inhibit migration at subtoxic concentrations; in vivo, CasIIIia and Cisplatin reduce tumor growth and metastatic foci, with Casiopeínas® reported as more efficient in cell viability assays.\"}]","Antitumoral and Antimetastatic Activity by Mixed Chelate Copper(II) Compounds (Casiopeínas®) on Triple-Negative Breast Cancer - In Vitro and In Vivo Models | PDF",1790033425,48]