[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-377655-105":59,"doc-detail-377655-en":131},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":124,"head_meta":126,"extra_data":128,"updated_unix":130},105,"en","antisense-lncrna-chromr-is-linked-to-glioma-patient-survival","Antisense lncRNA CHROMR is linked to glioma patient survival","","Natural non-coding antisense transcripts (ncNATs) are long non-coding RNAs transcribed from the opposite strand of a gene and can alter the expression of their sense partners through stabilization, miRNA absorption, or protection from degradation, or by promoting degradation and inhibiting translation. This original research investigates CHROMR and its sense gene PRKRA in gliomas, comparing tumor and normal brain expression and assessing survival associations. RNA-seq data from 26 patients were validated using TCGA and GTEx.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/antisense-lncrna-chromr-is-linked-to-glioma-patient-survival/377655/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/antisense-lncrna-chromr-is-linked-to-glioma-patient-survival/377655.png","ImageObject",300,407,{"name":92,"@type":93},"Logic","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-02","2026-09-24",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",11,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What was the study investigating in gliomas?","Question",{"text":113,"@type":114},"The study evaluated expression of the natural antisense lncRNA CHROMR and its sense protein-coding gene PRKRA in glioma and normal brain tissue, and assessed how CHROMR relates to patient survival.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How were gene expression levels measured and validated?",{"text":118,"@type":114},"RNA-seq was performed on post-surgical tumor samples from 26 glioma patients and normal brain tissue. CHROMR and PRKRA expression (TPM values) were then validated using TCGA and GTEx gene expression databases.",{"name":120,"@type":111,"acceptedAnswer":121},"What relationship did the study find between CHROMR and survival?",{"text":122,"@type":114},"CHROMR ncNAT expression was higher in glioma than in healthy brain. Survival analysis showed lower survival rates in patients with a low PRKRA/CHROMR mRNA/lncRNA expression ratio, linking elevated CHROMR levels to poor prognosis.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},377655,1790270452,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":44,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},1099513958762,"https://ap-avatar.wpscdn.com/avatar/1000023916a998db790?x-image-process=image/resize,m_fixed,w_180,h_180&k=1784791008015729253","TYPE Original Research PUBLISHED 06 March 2023  \nDOI 10.3389/fmolb.2023.1101953  \nOPEN ACCESS  \nEDITED BY  \nTominori Kimura,  \nRitsumeikan University, Japan  \nREVIEWED BY  \nMikio Nishizawa, Ritsumeikan University, Japan Tokifumi Odaka,  \nKansai Medical University, Japan  \n*CORRESPONDENCE  \nPaulina Vaitkienė,  \n [paulina.vaitkiene@lsmu.lt](paulina.vaitkiene@lsmu.lt)  \nSPECIALTY SECTION  \nThis article was submitted to RNA  \nNetworks and Biology, a section of the journal  \nFrontiers in Molecular Biosciences  \nRECEIVED 18 November 2022  \nACCEPTED 21 February 2023  \nPUBLISHED 06 March 2023  \nCITATION  \nŠirvinskas D, Steponaitis G, Stakaitis R, Tamašauskas A, Vaitkienė P and Skiriutė D (2023), Antisense lncRNA CHROMR is linked to glioma patient survival.  \nFront. Mol. Biosci. 10:1101953 .  \ndoi: 10.3389/fmolb.2023.1101953  \nCOPYRIGHT  \n© 2023 Širvinskas, Steponaitis, Stakaitis, Tamašauskas, Vaitkienė and Skiriutė . This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.  \nAntisense lncRNA CHROMR is linked to glioma patient survival  \nDovydas Širvinskas1, Giedrius Steponaitis 2, Rytis Stakaitis 2, Arimantas Tamašauskas2, Paulina Vaitkienė1* and Daina Skiriutė2  \n1Laboratory of Molecular Neurobiology, Neuroscience Institute, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania, 2Laboratory of Molecular Neurooncology, Neuroscience Institute, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania  \nBackground: Natural non-coding antisense transcripts (ncNATs) are long noncoding RNAs (lncRNA) transcribed from the opposite strand of a separate protein coding or non-coding gene. As such, ncNATs can increase overlapping mRNA (and the coded protein) levels by stabilizing mRNA, absorbing inhibitory miRNAsand protecting the mRNA from degradation, or conversely decrease mRNA (or protein) levels by directing the mRNA towards degradation or inhibiting protein translation. Recently, growing numbers of ncNATs were shown to be dysregulated in cancerous cells, however, actual impact of ncNATs on cancer progression remains largely unknown. We therefore investigated gene expression levels of natural antisense lncRNA CHROMR (Cholesterol Induced Regulator of Metabolism RNA) and its sense protein coding gene PRKRA (Protein Activator of Interferon Induced Protein Kinase EIF2AK2) in gliomas. Next, we checked CHROMR effect on the survival of glioma patients.  \nMethods: We performed RNA-seq on post-surgical tumor samples from 26 glioma patients, and normal brain tissue. Gene expression in TPM values were extracted for CHROMR and PRKRA genes. These data were validated using the TCGA and GTEx gene expression databases.  \nResults: The gene expression level of ncNAT lncRNA CHROMR in glioma tissue was significantly higher compared to healthy brain tissue, while the expression of its sense counterpart protein coding PRKRA mRNA did not differ between glioma and healthy samples. Survival analysis showed lower survival rates in patients with low mRNA PRKRA/lncRNA CHROMR gene expression ratio compared to high ratio showing a link between lncRNA CHROMR and glioma patient survival prognosis.  \nConclusion: Here we show that elevated levels of lncRNA CHROMR (i. e., low ratio of mRNA PRKRA/lncRNA CHROMR) is associated with poor prognosis for glioma patients.  \nKEYWORDS  \nncNATs, lncRNA, CHROMR, PRKRA, glioma, LGG, GBM, survival  \n1 Introduction  \nNatural antisense transcripts (NATs) are coding or non-coding RNA (ncRNA) sequences that are transcribed from the opposite strand of non-protein coding (nc) or protein coding (pc) genes and are comple","cbCaibrHt8xxyb0a","https://ap.wps.com/l/cbCaibrHt8xxyb0a","pdf",1352770,"English","# Background\n# Methods\n# Results\n# Conclusion\n# Keywords","[{\"question\":\"What was the study investigating in gliomas?\",\"answer\":\"The study evaluated expression of the natural antisense lncRNA CHROMR and its sense protein-coding gene PRKRA in glioma and normal brain tissue, and assessed how CHROMR relates to patient survival.\"},{\"question\":\"How were gene expression levels measured and validated?\",\"answer\":\"RNA-seq was performed on post-surgical tumor samples from 26 glioma patients and normal brain tissue. CHROMR and PRKRA expression (TPM values) were then validated using TCGA and GTEx gene expression databases.\"},{\"question\":\"What relationship did the study find between CHROMR and survival?\",\"answer\":\"CHROMR ncNAT expression was higher in glioma than in healthy brain. Survival analysis showed lower survival rates in patients with a low PRKRA/CHROMR mRNA/lncRNA expression ratio, linking elevated CHROMR levels to poor prognosis.\"}]","Antisense lncRNA CHROMR is linked to glioma patient survival | PDF",1790226473,23]