[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-443396-105":59,"doc-detail-443396-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","antioxidants-rescue-murine-mesangial-cells-from-docosahexaenoic-acid-induced-ferroptosis","Antioxidants rescue murine mesangial cells from docosahexaenoic acid-induced ferroptosis","","Omega-3 polyunsaturated fatty acids (n-3 PUFAs) are linked to anti-inflammatory effects, yet their molecular impact on kidney health remains insufficiently studied. This work examines whether docosahexaenoic acid (DHA) protects murine mesangial cells. DHA alone reduced viability and triggered ferroptosis-associated protein changes with lipid peroxidation and limited apoptosis. Ferrostatin-1 and an antioxidant cocktail preserved metabolism, prevented death, and increased TXNRD1 and GPX4 while lowering pro-ferroptotic factors.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/antioxidants-rescue-murine-mesangial-cells-from-docosahexaenoic-acid-induced-ferroptosis/443396/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/antioxidants-rescue-murine-mesangial-cells-from-docosahexaenoic-acid-induced-ferroptosis/443396.png","ImageObject",300,407,{"name":92,"@type":93},"Theodore","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-01","2026-09-29",true,{"@type":102,"interactionType":103,"userInteractionCount":14},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"Why study omega-3 PUFAs and kidney health at the molecular level?","Question",{"text":112,"@type":113},"Although omega-3 PUFAs provide multiple health benefits, few studies investigate their molecular effects in kidney health, and the mechanisms behind reported benefits are still unclear.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What happens to murine mesangial cells when treated with DHA alone?",{"text":117,"@type":113},"DHA stimulation alone decreases cell viability and leads to cell death, accompanied by ferroptosis-associated protein regulation and pronounced lipid peroxidation.",{"name":119,"@type":110,"acceptedAnswer":120},"How do antioxidants or ferroptosis inhibition affect DHA-induced cytotoxicity?",{"text":121,"@type":113},"The ferroptosis inhibitor ferrostatin-1 maintains metabolic activity and prevents cell death. An antioxidant cocktail similarly attenuates cell death and increases anti-ferroptotic proteins such as TXNRD1 and GPX4 while reducing pro-ferroptotic proteins.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},443396,1790865248,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":14,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},7971461740886,"https://ap-avatar.wpscdn.com/davatar_3d24733baf745e90a7e4bdd5f77d97b2","Saschin et al. Molecular and Cellular Pediatrics (2026) 13:2  \n[https://doi.org/10.1186/s40348-025-00215-y](https://doi.org/10.1186/s40348-025-00215-y)  \nMolecular and Cellular Pediatrics  \nRESEARCH Open Access  \nAntioxidants rescue murine mesangial cells from docosahexaenoic acid-induced ferroptosis  \nLeon Saschin 1, Anna Kowalewski 1, Gregor Fink 1, Jenny Voggel 1, Maria Wohlfarth 1, Lea Quell 1, Jörg Dötsch 1, Miguel A. Alejandre Alcázar 1,2,3,4, Kai-Dietrich Nüsken 1 and Eva Nüsken 1*  \nAbstract  \nBackground Omega-3 polyunsaturated fatty acids (n-3 PUFAs) are associated with anti-inflammatory effects. However, few studies have investigated their molecular effects in the kidney. We have previously shown that intrauterine growth restriction (IUGR) can lead to aggravation of mesangioproliferative glomerulonephritis and that n-3 PUFAs can attenuate long-term effects of IUGR by reversing pro-inflammatory molecular signatures in kidney cortex.  \nResults The original aim of the study was to investigate the potential protective mechanisms of docosahexaenoic acid (DHA) on murine mesangial cells. However, stimulation with DHA alone led to reduced cell viability and ultimately cell death. Proteome analysis revealed significant regulation of ferroptosis-associated proteins. Increased expression of the pro-ferroptotic protein HMOX1 and decreased expression of the pro-ferroptotic proteins TFRC and ACSL4 could indicate the onset of self-protection mechanisms in ferroptosis that is already underway. Interestingly, treatment with the ferroptosis inhibitor ferrostatin-1 maintained cellular metabolic activity and prevented cell death, further supporting a role of ferroptosis in DHA-induced cytotoxicity. Consistently, DHA-treated cells exhibited pronounced lipid peroxidation while showing no relevant activation of apoptosis. Simultaneous treatment with DHA and an antioxidant cocktail significantly attenuated cell death and induced upregulation of several key anti-ferroptotic proteins, including TXNRD1 and GPX4, while pro-ferroptotic proteins such asTFRC and ASCL4 were further reduced. Conclusion Our results provide evidence that DHA-treatment alone may have detrimental effects in susceptible cells, which could partially explain inconsistent results of clinical studies. This emphasizes the importance of a balance between pro-and anti-ferroptotic mechanisms in therapeutic strategies using n-3 PUFAs to promote kidney health. Keywords Ferroptosis, Omega-3 polyunsaturated fatty acids, Kidney health, Antioxidants, Oxidative stress  \n*Correspondence: Eva Nüsken [Eva.Nuesken@uk-koel n.de](Eva.Nuesken@uk-koel n.de)  \n1Department of Pediatric and Adolescent Medicine, Faculty of Medicine, University Hospital Cologne, University of Cologne, Kerpener Str. 62, 50937 Cologne, Germany  \n2Department of Pediatric and Adolescent Medicine, Translational Experimental Pediatrics, Experimental Pulmonology, Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne, Germany  \n3Cologne Excellence Cluster on Cellular Stress Responses in AgingAssociated Diseases (CECAD) and Center for Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, Germany  \n4Institute for Lung Health, University of Giessen and Marburg Lung Center (UGMLC), Member of the German Center for Lung Research (DZL), Gießen, Germany  \n© The Author(s) 2025. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended u","cbCaih20nqpgDPzT","https://ap.wps.com/l/cbCaih20nqpgDPzT","pdf",4484102,17,"English","# Abstract\n## Background\n## Results\n## Conclusion\n# Keywords\n# Background\n## Evidence from clinical and animal studies\n## Rationale and knowledge gaps","[{\"question\":\"Why study omega-3 PUFAs and kidney health at the molecular level?\",\"answer\":\"Although omega-3 PUFAs provide multiple health benefits, few studies investigate their molecular effects in kidney health, and the mechanisms behind reported benefits are still unclear.\"},{\"question\":\"What happens to murine mesangial cells when treated with DHA alone?\",\"answer\":\"DHA stimulation alone decreases cell viability and leads to cell death, accompanied by ferroptosis-associated protein regulation and pronounced lipid peroxidation.\"},{\"question\":\"How do antioxidants or ferroptosis inhibition affect DHA-induced cytotoxicity?\",\"answer\":\"The ferroptosis inhibitor ferrostatin-1 maintains metabolic activity and prevents cell death. An antioxidant cocktail similarly attenuates cell death and increases anti-ferroptotic proteins such as TXNRD1 and GPX4 while reducing pro-ferroptotic proteins.\"}]","Antioxidants rescue murine mesangial cells from docosahexaenoic acid-induced ferroptosis | PDF",1790703880,43]