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This research investigates the antibacterial potential and chemical composition of shiitake extracts prepared with 95% ethanol, ethyl acetate, and chloroform. Extraction yield was highest with 95% ethanol (31.16%). Antimicrobial activity was assessed against nine pathogenic isolates using paper disc diffusion, with ethyl acetate showing the strongest inhibition, followed by results supported by MIC and MBC assays. GC–MS identified key antimicrobial compounds including ergosterol and linoleic acid, supporting applications in food preservation, nutraceuticals, and pharmaceuticals.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/antibacterial-potential-and-chemical-composition-of-shiitake-mushroom-extract-against-pathogenic-bacteria/458928/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/antibacterial-potential-and-chemical-composition-of-shiitake-mushroom-extract-against-pathogenic-bacteria/458928.png","ImageObject",300,407,{"name":92,"@type":93},"A glass of water","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-30","2026-09-30",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"Which solvents were used to prepare the shiitake mushroom extracts?","Question",{"text":112,"@type":113},"The study prepared extracts using 95% ethanol, ethyl acetate, and chloroform.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was antibacterial activity evaluated against the bacterial isolates?",{"text":117,"@type":113},"Antibacterial activity was measured using the paper disc diffusion method against nine pathogenic bacterial isolates, with incubation at 37°C for 24 h.",{"name":119,"@type":110,"acceptedAnswer":120},"What key compounds were identified by GC–MS and how do they relate to antimicrobial effects?",{"text":121,"@type":113},"GC–MS identified bioactive compounds such as ergosterol and linoleic acid, which are associated with antimicrobial properties and support the extracts’ antibacterial potential.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},458928,1791078196,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":52,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144,"preview_page_count":4},962090760832,"https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d","Wiley  \nScientifica  \nVolume 2025, Article ID 6089332, 10 pages [https://doi.org/10.1155/sci5/6089332](https://doi.org/10.1155/sci5/6089332)  \nResearch Article  \nAntibacterial Potential and Chemical Composition of Shiitake Mushroom (Lentinus edodes (Berk.) Sing.) Extract Against Pathogenic Bacteria  \nWaraporn Sutthisa , 1 Pearploy Kamlangmak , 1 and Nattaya Srisawad 2  \n1 Department of Biology, Faculty of Science, Mahasarakham University, Kantarawichai,  \nMahasarakham Province 44150, Tailand  \n2 Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom Province 73170, Tailand  \nCorrespondence should be addressed to Waraporn Sutthisa; [waraporn.s@msu.ac.th](waraporn.s@msu.ac.th)  \nReceived 7 May 2025; Revised 18 November 2025; Accepted 24 November 2025  \nAcademic Editor: Linton Munyai  \nCopyright © 2025 Waraporn Sutthisa et al. Scientifca published by John Wiley & Sons Ltd. Tis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.  \nShiitake mushrooms (Lentinus edodes (Berk.) Sing.) have been widely recognized for their bioactive properties, including antimicrobial activity. Tis study aimed to investigate the antibacterial potential and chemical composition of shiitake mushroom extracts prepared using diferent solvents (95% ethanol, ethyl acetate, and chloroform) . Te highest extraction yield (31.16%) was obtained with 95% ethanol. Te antimicrobial activity of the extracts was evaluated using the paper disc difusion method against nine pathogenic bacterial isolates, including Gram-positive (Bacillus cereus, Staphylococcus aureus, and S. aureus DMST20654) and Gram-negative (Escherichia coli, E. coli ATCC25922, Enterobacter cloacae, Pseudomonas aeruginosa, Serratia marcescens, and Salmonella enterica serovar Typhi ATCC16122) bacteria. Plates were incubated at 37°C for 24 h. All experiments were performed in triplicate. Te ethyl acetate extract exhibited the strongest antibacterial activity, with the largest inhibition zone observed for  \nE. coli ATCC25922 (30.00 ± 0.00 mm), followed by S. enterica serovar Typhi ATCC16122 (28.33 ± 2.00 mm) . Te minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays confrmed the superior antibacterial potential of the ethyl acetate extract, particularly against S. aureus DMST20654 (MIC 􀂈 1.95 mg/mL, MBC 􀂈 31.25 mg/mL) . Gas chromatography–mass spectrometry (GC–MS) analysis identifed key bioactive compounds, including ergosterol (62.38%,%Prob 62.6 in the chloroform extract) and linoleic acid (28.65%,%Prob 56.5 in the ethyl acetate extract), which are known for their antimicrobial properties. Te fndings highlight the potential of ethyl acetate-extracted shiitake mushroom compounds as natural antibacterial agents, with applications for food preservation, nutraceuticals, and pharmaceuticals.  \nKeywords: antimicrobial activity; bioactive compounds; ergosterol; gas chromatography–mass spectrometry (GC–MS); shiitake mushroom  \n1. Introduction  \nShiitake mushroom (Lentinus edodes (Berk.) Sing.) is an aromatic and widely recognized edible mushroom, commonly used in various cuisines, both in fresh and dried forms. It has a soft texture, a distinctive umami favor, and is frequently included in vegetarian dishes. Beyond its culinary  \nvalue, shiitake mushrooms have been historically regarded for their medicinal benefts. In traditional Chinese medicine, they are considered a longevity enhancing food, believed to stimulate the immune system, combat bacterial infections, alleviate colds, and improve blood circulation [1] . Additionally, they have been associated with protective efects against heart disease, tumor growth, viral infections, and  \n2 Scientifca  \neven snake venom toxicity. Shiitake mushrooms are nutritionally rich, containing essential amino acids, including eritadenine, which aids in cholesterol metaboli","cbCaijxKd0aLLNfb","https://ap.wps.com/l/cbCaijxKd0aLLNfb","pdf",695035,"English","# Antibacterial Potential and Chemical Composition of Shiitake Mushroom Extract Against Pathogenic Bacteria\n## Introduction","[{\"question\":\"Which solvents were used to prepare the shiitake mushroom extracts?\",\"answer\":\"The study prepared extracts using 95% ethanol, ethyl acetate, and chloroform.\"},{\"question\":\"How was antibacterial activity evaluated against the bacterial isolates?\",\"answer\":\"Antibacterial activity was measured using the paper disc diffusion method against nine pathogenic bacterial isolates, with incubation at 37°C for 24 h.\"},{\"question\":\"What key compounds were identified by GC–MS and how do they relate to antimicrobial effects?\",\"answer\":\"GC–MS identified bioactive compounds such as ergosterol and linoleic acid, which are associated with antimicrobial properties and support the extracts’ antibacterial potential.\"}]","Antibacterial Potential and Chemical Composition of Shiitake Mushroom Extract Against Pathogenic Bacteria | PDF",1790754775,25]