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This study analyzed TCGA and GEO gastric adenocarcinoma data to assess prognosis links, used UALCAN for clinical correlations, and applied GO/KEGG/GSEA for function and pathway enrichment. TIMER2 evaluated immune infiltration associations. Validation used qPCR and immunohistochemistry.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/analyzing-the-expression-and-clinical-significance-of-cenpe-in-gastric-cancer/339289/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/analyzing-the-expression-and-clinical-significance-of-cenpe-in-gastric-cancer/339289.png","ImageObject",300,407,{"name":92,"@type":93},"Emma Wilson","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-28","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":29},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"How was CENPE-related prognosis evaluated in gastric cancer?","Question",{"text":112,"@type":113},"CENPE expression and patient outcomes were assessed using GEPIA based on TCGA and GEO gastric adenocarcinoma data, showing higher CENPE associated with worse prognosis.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What experimental methods were used to validate CENPE findings?",{"text":117,"@type":113},"The study validated CENPE overexpression using real-time quantitative PCR (qRT-PCR) and immunohistochemical analysis.",{"name":119,"@type":110,"acceptedAnswer":120},"What is the relationship between CENPE expression and immune cells in the gastric cancer microenvironment?",{"text":121,"@type":113},"CENPE expression correlated with immune-infiltrating cells, with positive links reported for NK cells and mast cells, supported by TIMER2-based analysis.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},339289,1790162099,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":29,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":41},3848291630094,"https://eur-avatar.wpscdn.com/davatar_085a072bc5b1113ac321206ff7593b45","Wang et al. BMC Medical Genomics (2024) 17:119 [https://doi.org/10.1186/s12920-024-01887-7](https://doi.org/10.1186/s12920-024-01887-7)  \nBMC Medical Genomics  \n RESEARCH Open Access  \nAnalyzing the expression and clinical significance of CENPE in gastric cancer  \nJing Wang1, Xiaofei Li1, Xihui Qiang1, Xueqing Yin1 and Lianyi Guo1*  \nAbstract  \nBackground Gastric cancer (GC) is a prevalent type of malignant gastrointestinal tumor. Many studies have shown that CENPE acts as an oncogene in some cancers. However, its expression level and clinical value in GC are not clear. Methods Obtaining clinical data information on gastric adenocarcinoma from TCGA and GEO databases. The gene expression profiling interaction analysis (GEPIA) was used to evaluate the relationship between prognosis and CENPE expression in gastric cancer patients. Utilizing the UALCAN platform, the correlation between CENPE expression and clinical parameters was examined. Functions and signaling pathways of CENPE were analyzed using the Gene Ontology (GO), the Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA) . The association between immunological infiltrating cells and CENPE expression was examined using TIMER2 .0. Validation was performed by real-time quantitative PCR (qPT-PCR) and immunohistochemical analysis.  \nResults According to the analysis of the GEPIA database, the expression of CENPE is increased in gastric cancer tissues compared to normal tissues. It was also found to have an important relationship with the prognosis of the patient (p\u003C0 . 05) . The prognosis was worse and overall survival was lower in individuals with increased expression of CENPE. In line with the findings ofthe GEPIA, real-time fluorescence quantitative PCR (qPT-PCR) confirmed that CENPE was overexpressed in gastric cancer cells. Furthermore, It was discovered that H. pylori infection status and tumor grade were related to CENPE expression. Enrichment analysis revealed that CENPE expression was linked to multiple biological functions and tumor-associated pathways. CENPE expression also correlated with immuneinfiltrating cells in the gastric cancer microenvironment and was positively connected to NK cells and mast cells. According to immunohistochemical examination, paracancerous tissues had minimal expression of CENPE, but gastric cancer showed significant expression of the protein.  \nConclusions According to our findings, CENPE is substantially expressed in GC and may perhaps contribute to its growth. CENPE might be a target for gastric cancer therapy and a predictor of a bad prognosis.  \nKeywords CENPE, Gastric cancer, Bioinformatics  \n*Correspondence: Lianyi Guo [angel_gly@163.com](angel_gly@163.com)  \n1 Department of Gastroenterology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121001, China  \nIntroduction  \nGastric cancer (GC), which positions fourth in death and fifth in incidence worldwide, is one of the most deadly diseases, with approximately 7,690,000 new diagnosesin 2020 [1]. It affects males six times more than women [1]. Despite significant progress in research on GC, the majority of cases are still identified at the late stages of the disease due to multiple risk factors and molecular mechanisms that are not yet fully understood. Radiation, chemotherapy, surgery, and targeted therapy are  \n© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence a","cbCaio7DUx0j6bUG","https://ap.wps.com/l/cbCaio7DUx0j6bUG","pdf",8807539,12,"English","# Abstract\n## Background\n## Methods\n## Results\n## Conclusions\n# Introduction","[{\"question\":\"How was CENPE-related prognosis evaluated in gastric cancer?\",\"answer\":\"CENPE expression and patient outcomes were assessed using GEPIA based on TCGA and GEO gastric adenocarcinoma data, showing higher CENPE associated with worse prognosis.\"},{\"question\":\"What experimental methods were used to validate CENPE findings?\",\"answer\":\"The study validated CENPE overexpression using real-time quantitative PCR (qRT-PCR) and immunohistochemical analysis.\"},{\"question\":\"What is the relationship between CENPE expression and immune cells in the gastric cancer microenvironment?\",\"answer\":\"CENPE expression correlated with immune-infiltrating cells, with positive links reported for NK cells and mast cells, supported by TIMER2-based analysis.\"}]","Analyzing the expression and clinical significance of CENPE in gastric cancer | PDF",1790029255]