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Public gene expression and clinical datasets were used to assess survival outcomes via survival analysis, multifactor Cox regression, enrichment, and mutation analyses, followed by experimental verification. Higher SRD5A3 expression in tumor tissues predicts worse overall survival and shorter progression-free survival, acting as an independent risk factor. SRD5A3 is inversely associated with CD4+ T, CD8+ T, and B-cell infiltration, and enrichment links it to signaling and tumor metastasis pathways; SRD5A3 knockdown reduces LIHC migration, invasion, and proliferation.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & 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is the main goal of this study on SRD5A3 in LIHC?","Question",{"text":63,"@type":64},"To determine how steroid 5 alpha-reductase 3 (SRD5A3) affects patient prognosis and the immune microenvironment in liver hepatocellular carcinoma (LIHC), using bioinformatics and in vitro validation.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How does SRD5A3 expression relate to survival outcomes in LIHC patients?",{"text":68,"@type":64},"Higher SRD5A3 expression is associated with poor overall survival and shorter progression-free survival, and multivariate Cox analysis identifies SRD5A3 as an independent risk factor.",{"name":70,"@type":61,"acceptedAnswer":71},"What is the relationship between SRD5A3 and immune cell infiltration?",{"text":72,"@type":64},"SRD5A3 expression is negatively correlated with immune infiltration of CD4+ T cells, CD8+ T cells, and B cells in LIHC.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},332547,1790198665,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,110,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & 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1, 2408463  \n[https://doi.org/10.1080/07853890.2024.2408463](https://doi.org/10.1080/07853890.2024.2408463)  \nReseaRch aRticle    \nAnalysis of the implication of steroid 5 alpha-reductase 3 on prognosis and immune microenvironment in Liver Hepatocellular Carcinoma  \nYuming lua‡ , Ziwei liub‡, Yu Zhengc‡, Xuesong liud, XiaoQin liuc, Nanguan chene, Kai Maoc and Weida linc   \nadepartment of Biostatistics, college of science, city University of Hong Kong, Hong Kong, china; bschool of nursing, Guangzhou Medical University, Guangzhou, Guangdong, china; cdepartment of Hepatobiliary Pancreatic surgery, shenshan Medical center, Memorial Hospital of sun Yat-sen University, shanwei, Guangdong, china; ddepartment of immunology, BinZhou Medical University, Binzhou, shandong, china; eluoding Hospital of Traditional chinese Medicine, luoding, Guangdong, china  \nABSTRACT  \nIntroduction: this study combined the bioinformatics and in vitro experiment-related technologies to analyze the impact of steroid 5 alpha-reductase 3 (sRD5a3) on the prognosis and immune microenvironment of liver hepatocellular carcinoma (lihc).  \nMethod: Gene expression and clinical data were obtained from public databases. the prognosis was evaluated using survival, multifactor cox, enrichment, and mutation analyses. this was then verified through in vitro experiments.  \nResults: the expression level of SRD5A3 in lihc tissues was significantly higher than that in the adjacent tissues. Kaplan–Meier survival analysis showed that high SRD5A3 expression was associated with poor overall survival (Os) and short progression-free survival in patients with lihc. Multivariate cox regression analysis revealed that positive SRD5A3 expression was an independent risk factor for Os in patients with lihc. expression of SRD5A3 was negatively correlated with immune cell infiltration of cD4+ t, cD8+ t, and B cells. GO and KeGG enrichment analyses showed that SRD5A3 was significantly enriched in signaling-and tumor metastasis-related pathways. Nomogram and calibration curve showed that the predicted performance of the model was consistent with the actual results. In vitro results confirmed that SRD5A3 knockdown inhibited the migration, invasion, and proliferation of lihc cells.  \nConclusions: SRD5A3 is actively expressed in lihc, and positive expression of SRD5A3 is an independent risk factor for different prognoses in patients with lihc. SRD5A3 can promote the proliferation, migration, and invasion of liver cancer cells and is related to short immune infiltration in patients with lihc.  \nKEY MESSAGES  \n1. high SRD5A3 expression is significantly associated with poor prognosis in patients with lihcand may help assess tumor progression.  \n2. highly expressed SRD5A3 promotes migration, invasion, and proliferation of liver cancer cells.  \n3. SRD5A3 expression is related to the degree of tumor immune cell infiltration.  \nARTICLE HISTORY  \nReceived 14 October 2023  \nRevised 2 May 2024 accepted 2 May 2024  \nKEYWORDS  \nsteroid 5 alpha-reductase 3; liver hepatocellular carcinoma; single cell sequencing; prognosis; immunotherapy  \n1. Introduction  \nliver hepatocellular carcinoma (lihc) is the most common cause of cancer-related deaths worldwide [1,2] . Most patients are diagnosed with lihc at an advanced stage, and early detection and treatment can greatly improve survival rate of the patient [3] . therefore, identifying new biomarkers and further  \nexploring their possible pathogenesis are crucial for the diagnosis and treatment of lihc.  \ntreatment methods for liver cancer include surgical resection, radiotherapy, chemotherapy, interventional embolization, and targeted therapy. surgical treatment for early stage liver cancer typically has a curative effect [4] . Despite this, most patients miss the  \nCONTACT Kai Mao  [mkz31@163.com](mkz31@163.com); Weida lin  lwd7189@163.com  department of Hepatobiliary Pancreatic surgery, shenshan Medical center, Memorial Ho","cbCaigqXAtCj9QPN","https://ap.wps.com/l/cbCaigqXAtCj9QPN","pdf",3518075,15,"English","# Abstract\n## Introduction\n## Method\n## Results\n## Conclusions\n# Key Messages\n# Keywords\n# Introduction\n## Background and Clinical Need\n## Current Treatment Modalities\n## Tumor Microenvironment and Immune-Related Genes","[{\"question\":\"What is the main goal of this study on SRD5A3 in LIHC?\",\"answer\":\"To determine how steroid 5 alpha-reductase 3 (SRD5A3) affects patient prognosis and the immune microenvironment in liver hepatocellular carcinoma (LIHC), using bioinformatics and in vitro validation.\"},{\"question\":\"How does SRD5A3 expression relate to survival outcomes in LIHC patients?\",\"answer\":\"Higher SRD5A3 expression is associated with poor overall survival and shorter progression-free survival, and multivariate Cox analysis identifies SRD5A3 as an independent risk factor.\"},{\"question\":\"What is the relationship between SRD5A3 and immune cell infiltration?\",\"answer\":\"SRD5A3 expression is negatively correlated with immune infiltration of CD4+ T cells, CD8+ T cells, and B cells in LIHC.\"}]","Analysis of the Implication of Steroid 5 Alpha-Reductase 3 on Prognosis and Immune Microenvironment in Liver Hepatocellular Carcinoma | PDF",1789998654,38]