[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-332264-105":59,"doc-detail-332264-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","analysis-of-naproxen-activation-of-cell-death-pathways-in-colo320-cells","Analysis of naproxen activation of cell death pathways in Colo320 cells","","Colorectal cancer is a common, life-threatening disease, and current therapies can cause serious side effects, increasing the need for better options. This study evaluated whether naproxen sodium (NS) shows chemo-preventive activity by examining cancerous Colo320 and healthy CCD-18 cells treated with increasing NS concentrations. NS increased caspase-3 activity and reduced Colo320 survival without affecting CCD-18. NS also inhibited Colo320 migration and altered RNA sequencing profiles, including downregulation of mucin 5B, mucin 5AC, and S100A9, later validated by ELISA results.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/analysis-of-naproxen-activation-of-cell-death-pathways-in-colo320-cells/332264/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/analysis-of-naproxen-activation-of-cell-death-pathways-in-colo320-cells/332264.png","ImageObject",300,407,{"name":92,"@type":93},"Noah","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-29","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":14},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What was the main goal of the study on naproxen sodium (NS)?","Question",{"text":112,"@type":113},"To confirm chemo-preventive potential of NS and to investigate molecular pathways explaining its effects on cell death and cancer phenotypes in Colo320 cells.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How did NS affect caspase-3 activity in Colo320 versus CCD-18 cells?",{"text":117,"@type":113},"NS significantly increased caspase-3 activity in Colo320 cells, while no significant change was reported in healthy CCD-18 cells.",{"name":119,"@type":110,"acceptedAnswer":120},"What did RNA sequencing and ELISA validation show after NS treatment?",{"text":121,"@type":113},"RNA sequencing showed statistically significant downregulation of genes including mucin 5B, mucin 5AC, and S100A9 after NS treatment, and these pathway findings were confirmed using ELISAs.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},332264,1792167301,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":14,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":34,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144,"preview_page_count":4},8796095462418,"https://ap-avatar.wpscdn.com/avatar/80000253c1241d02b47?x-image-process=image/resize,m_fixed,w_180,h_180&k=1778826106357471780","MOLECULAR AND CLINICAL ONCOLOGY 21: 61, 2024  \nAnalysis of naproxen activation of cell death pathways in Colo320 cells  \nANDREW CHEN 1,2 , WEI ZHU2,3 and RIAN GODING2,4  \n1College of Arts and Sciences at The State University of New York at Stony Brook, Stony Brook, NY 11794, USA; 2SCI Research Institute, Jericho, NY 11753, USA; 3Neuroscience Research Institute at The State University of New York at Old Westbury, Old Westbury,  \n4  \nNY 11568, USA; College of Osteopathic Medicine at The New York Institute of Technology, Old Westbury, NY 11568, USA  \nReceived September 17, 2023; Accepted June 7, 2024  \nDOI: 10. 3892/mco.2024.2759  \nAbstract. Colorectal cancer is a life‑threatening and prevalent type of cancer. However, a number of current treatments have serious side effects, which increase the need for alternatives. Non‑steroidal anti‑inflammatory drugs have potential chemo‑ preventive capabilities. The present study aimed to confirm this, as well as to investigate potential pathways and reasons for this trait. To accomplish this, cancerous Colo320 and healthy CCD‑18 cells were treated with various concentra‑ tions of naproxen sodium (NS) . A caspase‑3 assay revealed a statistically significant increase in caspase‑3 activity in Colo320 cells (300%; P\u003C0 .01), but not in CCD‑18 cells. This chemical was also associated with a significant decrease in Colo320 cell survival (‑72 . 888%; P\u003C0 .01), but not CCD‑18 cell survival. Furthermore, NS was found to significantly decrease the migration of Colo320 cells (86.58%; P\u003C0.01) . Finally, RNA sequencing of cells treated with NS revealed the statisti‑ cally significant downregulation of the mucin 5B, oligomeric mucus/gel‑forming, S100 calcium binding protein A9 and mucin 5AC, oligomeric mucus/gel‑forming genes, which are upregulated in colorectal cancer and are known to contribute to cancer proliferation, stemness and drug resistance. These novel biological pathway results were further confirmed using ELISAs. The present study identified a novel molecular mechanism of the anti‑colorectal cancer activity of NS.  \nIntroduction  \nColorectal cancer, also known as colon cancer, is a type of cancer that occurs in the colon or rectum. Cells that undergo mutation may grow and divide at an unusual rate and fail to undergo standard apoptosis . Thus, such cells may develop into unnatural growths known as polyps, which if left alone,  \nCorrespondence to: Dr Wei Zhu, Neuroscience Research Institute at The State University of New York at Old Westbury, 223 Store Hill Road, Old Westbury, NY 11568, USA  \nE‑mail: [zhuwei_69@yahoo.com](zhuwei_69@yahoo.com)  \nKey words: naproxen, colorectal cancer, colo320, nonsteroidal anti‑inflammatory drugs, ccd‑18, apoptosis  \ncan develop into cancerous tumors (1) . According to the American Society of Clinical Oncology, the current treatment methods for colorectal cancer can be categorized into three types: Surgeries, radiation therapies and medication thera‑ pies (2) . Surgeries typically involve the physical removal of the cancer and are also used to mitigate certain symptoms of colorectal cancer, such as in cases where the large intestine is blocked/damaged by cancer, preventing proper exocrine func‑ tion. However, such operations can also result in side effects such as constipation and diarrhea . Radiation therapy, on the other hand, removes cancer using high‑frequency waves. This is often used to complement surgeries, such as by removing small tumors that are difficult to remove surgically. Side effects range from fatigue to bleeding in the rectum. Finally, treatments with medication utilize various drugs to eliminate or prevent the replication of cancer cells through chemotherapy or targeted therapy. Some medications also aim to increase the capabilities of the immune system of the body. Side effects tend to be drug‑specific (2) .  \nNaproxen (NS) is typically known for its use as anon‑steroidal anti‑inflammatory drug (NSAID) and has been a subject of research due t","cbCaifpBwATVIAyX","https://ap.wps.com/l/cbCaifpBwATVIAyX","pdf",1848709,"English","# Abstract\n# Introduction\n# Materials and methods","[{\"question\":\"What was the main goal of the study on naproxen sodium (NS)?\",\"answer\":\"To confirm chemo-preventive potential of NS and to investigate molecular pathways explaining its effects on cell death and cancer phenotypes in Colo320 cells.\"},{\"question\":\"How did NS affect caspase-3 activity in Colo320 versus CCD-18 cells?\",\"answer\":\"NS significantly increased caspase-3 activity in Colo320 cells, while no significant change was reported in healthy CCD-18 cells.\"},{\"question\":\"What did RNA sequencing and ELISA validation show after NS treatment?\",\"answer\":\"RNA sequencing showed statistically significant downregulation of genes including mucin 5B, mucin 5AC, and S100A9 after NS treatment, and these pathway findings were confirmed using ELISAs.\"}]","Analysis of naproxen activation of cell death pathways in Colo320 cells | PDF",1789997601,18]