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Biomolecular condensates are increasingly recognized as membrane-less intracellular compartments that mediate cancer stress responses, yet in vivo evidence remains limited. This study uses human tissues and an orthotopic mouse model to examine the role of Amyloid-body, a stress-induced condensate, in tumorigenesis.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/amyloid-bodies-in-the-evolution-of-malignancies-research-article/351959/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/amyloid-bodies-in-the-evolution-of-malignancies-research-article/351959.png","ImageObject",300,407,{"name":92,"@type":93},"Blitz","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-02","2026-09-22",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",11,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What is the main focus of the study on amyloid-bodies?","Question",{"text":113,"@type":114},"The study examines how the Amyloid-body, a stress-induced membrane-less condensate, contributes to tumorigenesis in human tissues and an in vivo orthotopic mouse model.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How do the authors propose to visualize Amyloid-bodies in tumors?",{"text":118,"@type":114},"They use multiple immunohistochemical approaches and a semi-automated analysis pipeline to detect and assess Amyloid-bodies in tumor samples.",{"name":120,"@type":111,"acceptedAnswer":121},"What relationship is reported between Amyloid-bodies and tumor cell proliferation?",{"text":122,"@type":114},"Analysis across multiple tumor types shows Amyloid-bodies are detectable across tumor grades and stages, with a negative correlation with the proliferation marker Ki-67.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},351959,1790825683,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":145,"read_time":146},24464137899374,"https://us-avatar.wpscdn.com/davatar_3d24733baf745e90a7e4bdd5f77d97b2","OPEN ACCESS  \nCitation: Grunfeld AM, Bokros M, Paz K, Bilbao D, Lee S (2026) Amyloid-bodies in the evolution of malignancies. PLoS One 21(7): e0353464. [https://doi.org/10.1371/journal](https://doi.org/10.1371/journal). pone.0353464  \nEditor: Hualin Fu, Shanghai Jiao Tong University, CHINA  \nReceived: December 2, 2025  \nAccepted: June 22, 2026  \nPublished: July 9, 2026  \nCopyright: © 2026 Grunfeld et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.  \nData availability statement: The data generated in this study are available within the article and in the following Zenodo repository:  \n10.5281/zenodo.19947683.  \nFunding: This work was supported by NIH grants from the National Cancer Institute (R01CA275828) to SL, National Institute of  \nRESEARCH ARTICLE  \nAmyloid-bodies in the evolution of malignancies  \nAlexander Michael Grunfeld1,2,3,4, Michael Bokros2,3, Karelia Paz2,  \nDaniel Bilbao3,5, Stephen Lee1,2,3*  \n1 Sheila and David Fuente Graduate Program in Cancer Biology, Miller School of Medicine, University of Miami, Miami, Florida, United States of America, 2 Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Miami, Florida, United States of America, 3 Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, Florida, United States of America, 4 Medical Scientist Training Program, University of Miami Miller School of Medicine, Miami, Florida, United States of America, 5 Department of Pathology and Laboratory Medicine, University of Miami Miller School of Medicine, Miami, Florida, United States of America  \n* [stephenlee@med.miami.edu](stephenlee@med.miami.edu)  \nAbstract  \nTumorigenesis depends on the capacity for cancer cells to survive in the presence of various environmental stressors. An emerging paradigm in the study of cancer cell stress responses is their regulation by membrane-less intracellular compartments known as biomolecular condensates. While there has been considerable progress in our understanding of biomolecular condensates in cancer in vitro, a paucity of evidence remains for their presence and function in settings which more closely reflect in vivo tumor physiology. In this study, we use human tissues and an in vivo orthotopic mouse model to study the role of the Amyloid-body, a stress-induced condensate, in tumorigenesis. We present methodology to visualize Amyloid-bodies in tumors by multiple immunohistochemical approaches in addition to a semi-automated analysis pipeline. Analysis of multiple tumor types reveals that Amyloid-bodies are detectable at all tumor grades and stages, to varying degrees, and negatively correlate with the cell proliferation marker Ki-67 . Finally, in an orthotopic mouse model of breast cancer, we show that silencing long noncoding ribosomal intergenicspacer RNA (rIGSRNA) involved in Amyloid-body formation accelerates tumorigenesis in vivo. Together, these results suggest that Amyloid-body formation occurs inhuman cancers, further establishing the physiological relevance of biomolecular condensates.  \nIntroduction  \nCancer development is a multistep process which involves the gradual accumulation of genetic and epigenetic alterations resulting in aberrant cellular signaling [ 1] . Within the last decade, there has been a rapid increase in studies investigating how the mechanisms of this dysregulation are related to biomolecular condensates [2] . Biomolecular condensates are functional, membraneless compartments composed  \nPLOS One | [https://doi.org/10.1371/journal.pone.0353464](https://doi.org/10.1371/journal.pone.0353464) July 9, 2026 1 / 21  \nGeneral Medical Sciences (R35GM149221, R01GM115342, R01GM115342-S1) to SL, Sylvester Comprehensive Cancer Center to SL, National Institute of Aging (K99AG080474) to MB.  ","cbCaionlf2azG3k3","https://ap.wps.com/l/cbCaionlf2azG3k3","pdf",2853032,21,"English","# Abstract\n# Introduction","[{\"question\":\"What is the main focus of the study on amyloid-bodies?\",\"answer\":\"The study examines how the Amyloid-body, a stress-induced membrane-less condensate, contributes to tumorigenesis in human tissues and an in vivo orthotopic mouse model.\"},{\"question\":\"How do the authors propose to visualize Amyloid-bodies in tumors?\",\"answer\":\"They use multiple immunohistochemical approaches and a semi-automated analysis pipeline to detect and assess Amyloid-bodies in tumor samples.\"},{\"question\":\"What relationship is reported between Amyloid-bodies and tumor cell proliferation?\",\"answer\":\"Analysis across multiple tumor types shows Amyloid-bodies are detectable across tumor grades and stages, with a negative correlation with the proliferation marker Ki-67.\"}]","Amyloid-bodies in the evolution of malignancies - Research Article | PDF",1790096605,53]