[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-398697-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-398697-en":130},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","amycolatopsis-roodepoor-tensis-strain-ea7-antimicrobial-activity-and-bioactive-compounds-identification","Amycolatopsis roodepoor tensis strain EA7 - Antimicrobial activity and bioactive compounds identification","","Exploration of antimicrobial activity and identification of bioactive compounds from Amycolatopsis roodepoor tensis strain EA7. Actinomycetes were identified using biochemical and molecular methods, with 16S rRNA gene analysis showing 99.63% confidence for EA7. Ethyl acetate extract produced the largest inhibition zone against Gram-positive pathogens (25 mm) and showed MIC values from 312.5 µg/mL to 1250 µg/mL, while the methanolic extract had lower activity. GC-MS and LC-MS profiling revealed major compounds, including candidates with anticancer and antimicrobial potential.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/amycolatopsis-roodepoor-tensis-strain-ea7-antimicrobial-activity-and-bioactive-compounds-identification/398697/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/amycolatopsis-roodepoor-tensis-strain-ea7-antimicrobial-activity-and-bioactive-compounds-identification/398697.png","ImageObject",300,407,{"name":42,"@type":43},"McQueen","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-30","2026-09-27",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",8,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"How was strain EA7 identified in this study?","Question",{"text":63,"@type":64},"EA7 was identified using biochemical and molecular methods, and 16S rRNA gene analysis assigned it to Amycolatopsis roodepoor tensis with 99.63% confidence.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"Which extract showed the strongest antimicrobial effect?",{"text":68,"@type":64},"The ethyl acetate extract exhibited the strongest activity, including a 25 mm inhibition zone against Gram-positive pathogenic bacteria and MIC values ranging from 312.5 µg/mL to 1250 µg/mL.",{"name":70,"@type":61,"acceptedAnswer":71},"What kinds of compounds were identified using GC-MS and LC-MS?",{"text":72,"@type":64},"GC-MS identified compounds based on percentage area, retention time, molecular formula, molecular weight, and spectral quality; LC-MS identified nine major compounds with anticancer and antimicrobial activity. Major candidates mentioned include tetrangomycin, amycolactam, dihydroxybenzamide, dipyrimycin A, and tetracycline.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},398697,1790742718,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,110,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":108,"slug":109},7,40,"healthcare",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":111,"show_sort_weight":112,"slug":113},"Research & Report",30,"research-report",{"id":115,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},9,"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":97,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":107,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":139,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":145,"preview_page_count":4},5909890329169,"https://ap-avatar.wpscdn.com/davatar_9964176cb1d06d4a9deccf72a44ae3dc","Islamic Azad University, Lahijan Branch  \nIslamic Azad University, Lahijan Branch  \nIslamic Azad University, Lahijan Branch  \nIslamic Azad University, Lahijan Branch  \nAmycolatopsis, bioactive compounds, antimicrobial, GC-MS, LC-MS  July 19th, 2024  \n [https://doi.org/10.21203/rs.3.rs-4644566/v1](https://doi.org/10.21203/rs.3.rs-4644566/v1)  \n 􀁱 􀅏 This work is licensed under a Creative Commons Attribution 4.0 International License.  \nRead Full License  \n No competing interests reported.  \nThis paper delves into the antimicrobial activity and identi«cation of bioactive compounds of Amycolatopsis roodepoor tensis strain EA7 . Biochemical and molecular methods were utilized for the identi«cation of actinomycetes. One strain displaying superior antimicrobial activity was chosen for the identi«cation of bioactive compounds. The antimicrobial activity was thoroughly investigated. The analysis of the 16S rRNA gene revealed that strain EA7 belonged to the Amycolatopsis roodepoor tensis specie with 99 . 63% con«dence. The ethyl acetate extract exhibited the largest zone of inhibition against gram-positive pathogenic bacteria (25mm) using the disc diffusion method. In the MIC method, the ethyl acetate extract displayed the lowest MIC values ranging from 312.5 µg/mL ( S. aureus PTCC 1112) to 1250 µg/mL ( P. aeruginosa clinical and standard strain) . However, the methanolic extract showed lower antimicrobial activity. In the GC-MS analysis, compounds were identi«ed based on their percentage of area, retention time, molecular formula, molecular weight, and quality in the strain EA7 extract, with acetic acid, 2-methylpropyl ester (15 .8%) being the major compound. In the LC-MS analysis, nine major compounds with anticancer and antimicrobial activity were identi«ed. Among these, tetrangomycin, amycolactam, dihydroxybenzamide, and dipyrimycin A are compounds with potential anticancer activity, while tetracycline exhibits potential antimicrobial activity.  \nThe rising challenges posed by antibiotic-resistant pathogens, the emergence of new diseases, and the toxicity associated with current bioactive compounds have led to a growing demand for antibiotics worldwide (Adamek et al., 2018; Jha et al., 2022) . In particular, infections caused by multidrug-resistant (MDR) gram-negative pathogens present a signi«cant global challenge, highlighting the urgent need for the discovery of novel antimicrobial compounds and treatment approaches. Nature has long served as a valuable source of bioactive compounds with diverse applications in pharmaceuticals, medicine, and biochemistry (Djebbah et al., 2021) . Microorganisms, especially actinomycetes, have been instrumental in contributing to human health through the production of antibiotics. Among actinomycetes, the genus Streptomyces is a potential source of all secondary metabolites.Additionally, genera such as Amycolatopsis, Actinoplanes, Micromonospora, and Sacchar opolyspor a are vital producers of antibiotics (Singh, 2021) . Amycolatopsis, a member of the Pseudonocardiaceae family, consists of nocar dioform actinomycetes characterized by the absence of mycolic acids and the presence of aminopimelic acid, arabinose, and galactose mesodies. Notably, the cell wall peptidoglycan of Amycolatopsis is renowned for yielding antibiotic strains that produce ansamycin compounds, distinguishing them from other groups. Due to their versatility in producing antibiotics, anti-cancer agents, and other therapeutically important compounds, Amycolatopsis is considered to be among the key microorganisms of the 21st century (Adamek et al., 2018; Julianti et al., 2022) . This study focuses on the exploration of the lesserknown group of non-streptomycete actinobacteria, which remain underutilized despite their vast chemical diversity. These rare actinomycetes are known to produce a wide array of unique, often complex compounds with potent bioactivity and low toxicity. While research on the biosynthesis of secondary metabolite","cbCailLy0cU4CyvJ","https://ap.wps.com/l/cbCailLy0cU4CyvJ","pdf",1278359,17,"English","# Introduction\n## Background and motivation\n# Materials and Methods\n## Soil sampling and isolation\n## Culture conditions and contamination control\n## Biochemical and morphological characterization\n# Antimicrobial assays and bioactive compound analysis\n## Disc diffusion and MIC\n## GC-MS and LC-MS identification\n# Results and implications\n## Anticancer and antimicrobial candidate compounds","[{\"question\":\"How was strain EA7 identified in this study?\",\"answer\":\"EA7 was identified using biochemical and molecular methods, and 16S rRNA gene analysis assigned it to Amycolatopsis roodepoor tensis with 99.63% confidence.\"},{\"question\":\"Which extract showed the strongest antimicrobial effect?\",\"answer\":\"The ethyl acetate extract exhibited the strongest activity, including a 25 mm inhibition zone against Gram-positive pathogenic bacteria and MIC values ranging from 312.5 µg/mL to 1250 µg/mL.\"},{\"question\":\"What kinds of compounds were identified using GC-MS and LC-MS?\",\"answer\":\"GC-MS identified compounds based on percentage area, retention time, molecular formula, molecular weight, and spectral quality; LC-MS identified nine major compounds with anticancer and antimicrobial activity. Major candidates mentioned include tetrangomycin, amycolactam, dihydroxybenzamide, dipyrimycin A, and tetracycline.\"}]","Amycolatopsis roodepoor tensis strain EA7 - Antimicrobial activity and bioactive compounds identification | PDF",1790488202,43]