[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-416978-105":59,"doc-detail-416978-en":131},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":124,"head_meta":126,"extra_data":128,"updated_unix":130},105,"en","altered-high-density-lipoprotein-expression-pattern-in-the-aqueous-humor-from-eyes-with-age-related-macular-degeneration","Altered High-Density Lipoprotein Expression Pattern in the Aqueous Humor From Eyes With Age-Related Macular Degeneration","","Age-related macular degeneration (AMD) is linked to altered protein expression in aqueous humor (AH). This study performs an unbiased proteomic analysis of AH to define proteomic signatures for neovascular AMD (nAMD) and non-neovascular AMD (nnAMD) stages, using liquid chromatography-tandem mass spectrometry and functional characterization of differentially expressed proteins. HDL-associated pathway proteins are increased in nAMD, while nnAMD shows no differential proteins versus controls. HDL pathway proteins correlate negatively with serum HDL-C, suggesting serum levels may not reflect ocular HDL particle abundance.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/altered-high-density-lipoprotein-expression-pattern-in-the-aqueous-humor-from-eyes-with-age-related-macular-degeneration/416978/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/altered-high-density-lipoprotein-expression-pattern-in-the-aqueous-humor-from-eyes-with-age-related-macular-degeneration/416978.png","ImageObject",300,407,{"name":92,"@type":93},"Mafia Boss","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-31","2026-09-28",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",12,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What is the main goal of the study?","Question",{"text":113,"@type":114},"To perform an unbiased proteomic analysis of aqueous humor to identify disease-related proteomic signatures distinguishing neovascular and non-neovascular AMD stages.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How were AH protein profiles analyzed?",{"text":118,"@type":114},"Aqueous humor samples were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS), followed by functional analysis of differentially expressed proteins.",{"name":120,"@type":111,"acceptedAnswer":121},"What did the study find about HDL-associated proteins in nAMD?",{"text":122,"@type":114},"Proteins associated with the spherical HDL particle pathway are upregulated in nAMD aqueous humor, and they show a negative correlation with serum HDL-C levels.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},416978,1790760560,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":52,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},962090760730,"https://ap-avatar.wpscdn.com/davatar_9964176cb1d06d4a9deccf72a44ae3dc","Retina  \nAltered High-Density Lipoprotein Expression Pattern in the Aqueous Humor From Eyes With Age-Related Macular Degeneration  \nAdi Kramer,1 Batya Rinsky,1 Sarah Elbaz-Hayoun,1 Samer Khateb,1 Tareq Jaouni,1 Shlomit Jaskoll,1 Liran Tiosano,1 Ronen Durst,2 and Itay Chowers1  \n1Department of Ophthalmology, Hadassah Medical Center, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel  \n2 Cardiology Department, Hadassah-Hebrew University Medical Center, Jerusalem, Israel  \nCorrespondence: Itay Chowers, Department of Ophthalmology, Hadassah Medical Center, POB 12000, Jerusalem 91120, Israel; [chowers@hadassah.org.il](chowers@hadassah.org.il).  \nReceived: June 17, 2025  \nAccepted: October 24, 2025  \nPublished: December 30, 2025  \nCitation: Kramer A, Rinsky B, Elbaz-Hayoun S, et al. Altered high-density lipoprotein expression pattern in the aqueous humor from eyes with age-related macular degeneration. Invest Ophthalmol Vis Sci. 2025;66(15):73 .  \n[https://doi.org/10.1167/iovs.66.15.73](https://doi.org/10.1167/iovs.66.15.73)  \nPURPOSE. Age-related macular degeneration (AMD) is associated with altered protein expression in the aqueous humor (AH) . We aim to perform an unbiased proteomic analysis of the AH to identify proteomic signatures characterizing the disease’s neovascular AMD (nAMD) and non-neovascular AMD (nnAMD) stages.  \nMETHODS. AH samples were collected from eyes with nAMD (n = 39), nnAMD (n = 30), and healthy control eyes (n = 36) . Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to analyze the AH proteome; differentially expressed proteins underwent functional analysis. Serum high-density lipoprotein cholesterol (HDL-C) levels were correlated with AH high-density lipoprotein (HDL) pathway proteins.  \nRESULTS. Seventeen proteins were upregulated and five were downregulated in nAMD eyes compared with healthy controls. Enriched pathways include the fibrinogen complex, the complement alternate, the spherical HDL particle, and the serine protease inhibitor. Forty-six proteins were upregulated in nAMD versus nnAMD, whereas nine were downregulated. Enriched pathways included the spherical HDL particle, peptidase S1A, membrane attack complex, Sushi domain, and complement alternate pathway. No differentially expressed proteins were found between nnAMD and control eyes. Upregulated proteins associated with the spherical HDL particle pathway in nAMD, including apolipoprotein A-I, apolipoprotein A-II, and paraoxonase 1, exhibited a negative correlation with serum HDL-C levels (r = −0 .43, P = 0.012; r = −0 .37, P = 0.031; and r = −0.52, P = 0.002, respectively) .  \nCONCLUSIONS. HDL-associated proteins exhibit increased expression in AH of nAMD eyes, independent of serum HDL-C levels. These findings suggest that serum HDL-C may not accurately reflect ocular HDL particle levels, highlighting the role of retinal lipid dysregulation in nAMD.  \nKeywords: age-related macular degeneration (AMD), aqueous humor (AH), proteomics, high density lipoprotein (HDL), apolipoprotein A1 (ApoA1)  \nP roteomic analysis of involved tissues can provide  \nimportant insights into complex multifactorial diseases, such as age-related macular degeneration (AMD) . Recent advancements in proteomic technologies have made it possible to characterize tissue proteomes in an unbiased manner, and improvements in methodology and technology have made it possible to generate large datasets from minimal sample volumes. Aqueous humor (AH) proteomics reflects retinal metabolic and pathological processes. Several studies have identified alterations in AH protein composition associated with eye diseases, among them high myopia,1 glaucoma, 2 and diabetic retinopathy.3  \nAlthough AMD is localized to the macula, which constitutes less than 1% of the total fundus area, and the AH  \nreflects the broader intraocular environment, AH sampling remains one of the least invasive methods available for investigating biochemical changes within the","cbCaioZjIxmHjbk6","https://ap.wps.com/l/cbCaioZjIxmHjbk6","pdf",1559540,"English","# Purpose\n# Methods\n# Patients\n# Results\n# Conclusions","[{\"question\":\"What is the main goal of the study?\",\"answer\":\"To perform an unbiased proteomic analysis of aqueous humor to identify disease-related proteomic signatures distinguishing neovascular and non-neovascular AMD stages.\"},{\"question\":\"How were AH protein profiles analyzed?\",\"answer\":\"Aqueous humor samples were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS), followed by functional analysis of differentially expressed proteins.\"},{\"question\":\"What did the study find about HDL-associated proteins in nAMD?\",\"answer\":\"Proteins associated with the spherical HDL particle pathway are upregulated in nAMD aqueous humor, and they show a negative correlation with serum HDL-C levels.\"}]","Altered High-Density Lipoprotein Expression Pattern in the Aqueous Humor From Eyes With Age-Related Macular Degeneration | PDF",1790607513,25]