[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-332930-105":59,"doc-detail-332930-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","alterations-in-mirna-expression-and-their-role-in-the-pathogenesis-of-cervical-cancer","Alterations in miRNA Expression and Their Role in the Pathogenesis of Cervical Cancer","","Cervical cancer has high incidence and mortality, driven largely by high-risk HPV infection. The study investigates whether HPV-associated changes in microRNA (miRNA) expression contribute to cervical cancer pathogenesis. Transcriptome data were analyzed for miRNA and gene expression differences between normal tissues and tumors, using GEO2R with significance at p\u003C0.05. Predicted miRNA targets informed pathway network construction (Cytoscape) and cancer hallmarks mapping (COSMIC).",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/alterations-in-mirna-expression-and-their-role-in-the-pathogenesis-of-cervical-cancer/332930/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/alterations-in-mirna-expression-and-their-role-in-the-pathogenesis-of-cervical-cancer/332930.png","ImageObject",300,407,{"name":92,"@type":93},"Theodora","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-01","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the study’s main objective regarding miRNAs and cervical cancer?","Question",{"text":112,"@type":113},"To investigate changes in microRNA expression associated with cervical cancer and assess the biological significance of those changes induced by HPV proteins.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were miRNA and gene expression alterations identified?",{"text":117,"@type":113},"Transcriptome data from NCBI were analyzed using GEO2R to compare normal tissues and cervical cancer, selecting miRNAs and genes with significantly altered expression (p-value \u003C 0.05).",{"name":119,"@type":110,"acceptedAnswer":120},"Which downstream biological effects were linked to deregulated miRNAs?",{"text":121,"@type":113},"Deregulated genes associated with altered miRNAs produced changes across 52 biological pathways, affecting processes including interferon signaling, interleukin signaling, oxidative stress-induced senescence, cell-cycle checkpoints, and transcriptional regulation by P53.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},332930,1790013155,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":44,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144,"preview_page_count":4},687197207919,"https://ap-avatar.wpscdn.com/avatar/a000253d6f5f7c60be?x-image-process=image/resize,m_fixed,w_180,h_180&k=1779446848396160552","RESEARCH ARTICLE  \nEditorial Process: Submission:03/20/2024 Acceptance:10/18/2024  \nAlterations in miRNA Expression and Their Role in the Pathogenesis of Cervical Cancer  \nPaula Samyla Fernandes Feitosa1 *, Fernando Liberalino Fernandes1, Tauê Posada Pereira1, Daria Raquel Queiroz de Almeida1, Amanda Estevam Carvalho1, Valéria Duarte de Almeida1, Gilson Aquino Cavalcante1, José Antonio da Silva Júnior1, Tayline Dantas Rodrigues1, Ellany Gurgel Cosme do Nascimento1, Christiane Medeiros Bezerra2, José Veríssimo Fernandes2, Thales Allyrio Araújo de Medeiros Fernandes1  \nAbstract  \nBackground: Cervical cancer has a high incidence and mortality rate, affecting more than half a million women in 2018. Its development is strongly related to high-risk HPV infection. After infection, several cellular molecules are affected, including microRNAs (miRNAs), which are the focus of our study. We aimed to investigate changes in microRNA expression associated with cervical cancer and analyze the biological significance of these changes induced by HPV proteins. Methods: We analyzed transcriptome data retrieved from theNCBI website to investigate miRNA and gene expression in cervical cancer. We evaluated the alteration in expression of miRNAs and genes (between normal tissues and cervical cancer) using the GEO2R tool and selected those with significantly altered expression (p-value \u003C 0.05). The target genes of miRNAs were predicted using the miRNA Pathway Dictionary Database. Subsequently, we created a network of biological pathways affected by miRNA deregulation using Cytoscape software and associated the altered miRNAs with the Hallmarks of Cancer using COSMIC v84 . Results: We identified 10 miRNAs and 82 target genes with significantly altered expression levels in cervical cancer that matched the predicted results. In addition, the deregulation of these genes causes changes in 52 biological pathways. These miRNAs affected pathways such as interferon signaling (miR-106b-5p and miR-1183), signaling by interleukins (miR-557, miR-106b-5p, miR-15a-5p, and miR-21-5p), oxidative stress-induced senescence (miR-557 and miR-15a-5p), cell cycle checkpoints (miR-557), transcriptional regulation by P53 (miR-557 and miR-15a-5p), and the exchange of oxygen and carbon dioxide in erythrocytes (miR-15a-5p) . Conclusion: Alterations in miRNA expression play an important role in the pathogenesis of cervical cancer, affecting several biological pathways and Hallmarks of Cancer, such as immune system regulation, cell cycle regulation, and energy metabolism. Thus, their analysis can contribute to the development of diagnostic and prognostic biomarkers and more effective treatments for cervical cancer.  \nKeywords: MicroRNAs-Uterine Cervical Neoplasms-Human Papillomavirus Viruses  \nAsian Pac J Cancer Prev, 25 (10), 3533-3541  \nIntroduction  \nCervical cancer is the fourth most common cancer in women worldwide [1]. With large global variations in mortality rates, it is the leading cause of cancer-related death among women in 36 countries. In 2020, 604.127 new cases were diagnosed, with 341.831 deaths projected [2] . The development of cervical squamous cell carcinomas occurs through well-recognized premalignant precursor lesions, known as cervical intraepithelial neoplasia (CIN), graded 1-3, while the precursor stages preceding cervical adenocarcinomas are less well understood [3] .  \nThe pathogenic mechanisms of cervical cancer are initiated by high-risk human papillomavirus (hrHPV) infection. The HPV genome contains genes such as E1, E2, and E4-E7, which encode viral oncoproteins. Among these viral oncoproteins, E5, E6, and E7 deserve special mention. The E5 protein can act by activating the epidermal growth factor receptor (EGFR). The hr-HPVE6 protein binds to a component of the E3 family of ubiquitin ligases, called E6-AP (E6-associated protein ligase), and induces the formation of a complex with the p53 tumor suppressor protein, leading to genetic instability. E7 b","cbCaipV4rNU3sULj","https://ap.wps.com/l/cbCaipV4rNU3sULj","pdf",901720,"English","# Abstract\n# Background\n# Methods\n## Differential expression and statistical filtering\n## Target prediction and pathway construction\n## Hallmarks mapping\n# Results\n# Conclusion\n# Introduction","[{\"question\":\"What is the study’s main objective regarding miRNAs and cervical cancer?\",\"answer\":\"To investigate changes in microRNA expression associated with cervical cancer and assess the biological significance of those changes induced by HPV proteins.\"},{\"question\":\"How were miRNA and gene expression alterations identified?\",\"answer\":\"Transcriptome data from NCBI were analyzed using GEO2R to compare normal tissues and cervical cancer, selecting miRNAs and genes with significantly altered expression (p-value \\u003c 0.05).\"},{\"question\":\"Which downstream biological effects were linked to deregulated miRNAs?\",\"answer\":\"Deregulated genes associated with altered miRNAs produced changes across 52 biological pathways, affecting processes including interferon signaling, interleukin signaling, oxidative stress-induced senescence, cell-cycle checkpoints, and transcriptional regulation by P53.\"}]","Alterations in miRNA Expression and Their Role in the Pathogenesis of Cervical Cancer | PDF",1790000047,23]