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Basigin (CD147) is a type 1 integral membrane receptor secreted in exosomes or released by ectodomain shedding. This study examines how an ADAM12-generated basigin ectodomain supports cancer progression. Results show recombinant ectodomain binding to β1 integrin, promoting gelatin degradation and cancer cell migration through MMP- and β1-integrin-dependent mechanisms.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/adam12-generated-basigin-ectodomain-binds-1-integrin-and-enhances-the-expression-of-cancer-related-extracellular-matrix-proteins-abstract/332140/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/adam12-generated-basigin-ectodomain-binds-1-integrin-and-enhances-the-expression-of-cancer-related-extracellular-matrix-proteins-abstract/332140.png","ImageObject",300,407,{"name":92,"@type":93},"Hazel","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-24","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":29},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the role of basigin in aggressive cancer biology?","Question",{"text":112,"@type":113},"Basigin is a type 1 integral membrane receptor that can be released via exosomes or ectodomain shedding. Elevated soluble basigin is linked to poor cancer prognosis and relates to cancer progression processes such as invasion.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How does the ADAM12-generated basigin ectodomain influence cancer cell behavior?",{"text":117,"@type":113},"The recombinant ADAM12-generated basigin ectodomain binds β1 integrin and stimulates gelatin degradation and cancer cell migration. The effects occur in an MMP- and β1-integrin-dependent manner.",{"name":119,"@type":110,"acceptedAnswer":120},"What extracellular matrix changes were observed in vitro and in vivo?",{"text":121,"@type":113},"In vitro and in vivo experiments showed altered expression of extracellular matrix proteins, including fibronectin and collagen type 5. Tumors in nude mice bearing ADAM12-expressing MCF7 cells showed increased collagen type 5 deposits, mimicking desmoplasia-like stromal response.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},332140,1790809152,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":29,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},137441390410,"https://ap-avatar.wpscdn.com/avatar/2000252f4ab5702993?_k=1776741390130283984","Article  \nADAM12-Generated Basigin Ectodomain Binds β1 Integrin and Enhances the Expression of Cancer-Related Extracellular Matrix Proteins  \nKasper J. Mygind 1, Denise Nikodemus 1, Sebastian Gnosa 1, Ramya Kweder 1, Nicolai J. Wewer Albrechtsen 2, Marie Kveiborg 1, Janine T. Erler 1 and Reidar Albrechtsen 1, *  \nCitation: Mygind, K.J.; Nikodemus, D.; Gnosa, S.; Kweder, R.; Albrechtsen, N.J.W.; Kveiborg, M.; Erler, J.T.; Albrechtsen, R. ADAM12-Generated Basigin Ectodomain Binds β1 Integrin and Enhances the Expression of Cancer-Related Extracellular Matrix Proteins. Int. J. Mol. Sci. 2024, 25, 5871 . [https://doi.org/10.3390/ijms25115871](https://doi.org/10.3390/ijms25115871)  \n[Academic Editor: Zolt](Academic Editor: Zolt)án Wiener  \nReceived: 21 March 2024  \nRevised: 15 May 2024  \nAccepted: 25 May 2024  \nPublished: 28 May 2024  \nCopyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://](https://)[ ](https://)[creativecommons.org/licenses/by/](creativecommons.org/licenses/by/)[ ](creativecommons.org/licenses/by/)[4.0/](4.0/)) .  \n1 Biotech Research and Innovation Centre (BRIC), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark; [kmygind@sund.ku.dk](kmygind@sund.ku.dk) (K.J.M.);  \ndenise.nikodemus@biologie.uni-freiburg.de (D.N.); [seg@minervaimaging.com](seg@minervaimaging.com) (S.G.);  \n[ramya.kweder@sund.ku.dk](ramya.kweder@sund.ku.dk) (R.K.); [marie.kveiborg@sund.ku.dk](marie.kveiborg@sund.ku.dk) (M.K.); [janine.erler@bric.ku.dk](janine.erler@bric.ku.dk) (J.T.E.)  \n2 Department of Clinical Biochemistry, Copenhagen University Hospital-Bispebjerg, 2400 Copenhagen, Denmark; [nicolai.albrechtsen@regionh.dk](nicolai.albrechtsen@regionh.dk)  \n* Correspondence: [reidaral@sund.ku.dk](reidaral@sund.ku.dk)  \nAbstract: Desmoplasia is a common feature of aggressive cancers, driven by a complex interplay of protein production and degradation. Basigin is a type 1 integral membrane receptor secreted inexosomes or released by ectodomain shedding from the cell surface. Given that soluble basigin is increased in the circulation of patients with a poor cancer prognosis, we explored the putative role of the ADAM12-generated basigin ectodomain in cancer progression. We show that recombinant basigin ectodomain binds β1 integrin and stimulates gelatin degradation and the migration of cancer cells in a matrix metalloproteinase (MMP)-and β1-integrin-dependent manner. Subsequent in vitro and in vivo experiments demonstrated the altered expression of extracellular matrix proteins, including fibronectin and collagen type 5 . Thus, we found increased deposits of collagen type 5 in the stroma of nude mice tumors of the human tumor cell line MCF7 expressing ADAM12—mimicking the desmoplastic response seen in human cancer. Our findings indicate a feedback loop between ADAM12 expression, basigin shedding, TGFβ signaling, and extracellular matrix (ECM) remodeling, which could be a mechanism by which ADAM12-generated basigin ectodomain contributes to the regulation of desmoplasia, a key feature in human cancer progression.  \nKeywords: CD147/Basigin; disintegrin and metalloproteinase; extracellular matrix  \n1. Introduction  \nThe extracellular matrix metalloproteinase inducer (EMMPRIN), also termed CD147/ basigin, is a glycoprotein belonging to the immunoglobulin family [1,2] . Four variants (basigin 1–4) exist, but the most abundant and well-studied is isoform 2 (henceforth termed basigin) . Basigin (BSG) is a 269 amino acid long type I transmembrane protein composed of an N-terminal signal sequence, a 186 residue-long extracellular portion consisting of two Ig-like domains (D1 and D2), a transmembrane domain, and a C-terminal cytoplasmic domain [3] . It has been shown to exert several different functions at the plasma membrane, acting as a homo-or heterodimer in eith","cbCaim6Uud2Bd2WP","https://ap.wps.com/l/cbCaim6Uud2Bd2WP","pdf",2642995,15,"English","# Abstract\n## Basigin and desmoplasia in aggressive cancer\n## ADAM12-generated ectodomain mechanism\n## Effects on extracellular matrix proteins\n## Feedback loop model","[{\"question\":\"What is the role of basigin in aggressive cancer biology?\",\"answer\":\"Basigin is a type 1 integral membrane receptor that can be released via exosomes or ectodomain shedding. Elevated soluble basigin is linked to poor cancer prognosis and relates to cancer progression processes such as invasion.\"},{\"question\":\"How does the ADAM12-generated basigin ectodomain influence cancer cell behavior?\",\"answer\":\"The recombinant ADAM12-generated basigin ectodomain binds β1 integrin and stimulates gelatin degradation and cancer cell migration. The effects occur in an MMP- and β1-integrin-dependent manner.\"},{\"question\":\"What extracellular matrix changes were observed in vitro and in vivo?\",\"answer\":\"In vitro and in vivo experiments showed altered expression of extracellular matrix proteins, including fibronectin and collagen type 5. Tumors in nude mice bearing ADAM12-expressing MCF7 cells showed increased collagen type 5 deposits, mimicking desmoplasia-like stromal response.\"}]","ADAM12-Generated Basigin Ectodomain Binds β1 Integrin and Enhances the Expression of Cancer-Related Extracellular Matrix Proteins - Abstract | PDF",1789997001,38]