[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-363962-105":59,"doc-detail-363962-en":129},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":122,"head_meta":124,"extra_data":126,"updated_unix":128},105,"en","acetyltransferase-nat10-promotes-aggressiveness-of-colorectal-cancer-cells-via-ac4c-acetylation-of-serpina1-mrna","Acetyltransferase NAT10 promotes aggressiveness of colorectal cancer cells via ac4C acetylation of SERPINA1 mRNA","","N-acetyltransferase 10 (NAT10) catalyzes N4-acetylcytidine (ac4C) RNA modification and influences tumor development. This study examines whether NAT10 drives colorectal cancer progression through ac4C modification of SERPINA1 mRNA. NAT10 dysregulation was assessed in colorectal cancer using transcriptome data and RT-qPCR, while ac4C levels were quantified by dot blotting. Functional assays evaluated cell viability, proliferation, invasion, and migration, with mechanistic validation by RIP and luciferase reporter experiments. In vivo xenografts and pharmacological NAT10 inhibition further established a therapeutic axis via ac4C-dependent stabilization of SERPINA1.",{"@graph":69,"@context":121},[70,84,104],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/acetyltransferase-nat10-promotes-aggressiveness-of-colorectal-cancer-cells-via-ac4c-acetylation-of-serpina1-mrna/363962/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":98,"encodingFormat":97,"isAccessibleForFree":99,"interactionStatistic":100},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/acetyltransferase-nat10-promotes-aggressiveness-of-colorectal-cancer-cells-via-ac4c-acetylation-of-serpina1-mrna/363962.png","ImageObject",300,407,{"name":92,"@type":93},"kopisore","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-09-23",true,{"@type":101,"interactionType":102,"userInteractionCount":14},"InteractionCounter",{"@type":103},"ViewAction",{"@type":105,"mainEntity":106},"FAQPage",[107,113,117],{"name":108,"@type":109,"acceptedAnswer":110},"What role does NAT10 play in colorectal cancer according to the study?","Question",{"text":111,"@type":112},"NAT10 promotes colorectal cancer aggressiveness by regulating ac4C modification of SERPINA1 mRNA. Increased NAT10 and global ac4C levels were observed in colorectal cancer cells.","Answer",{"name":114,"@type":109,"acceptedAnswer":115},"How did the study measure ac4C modification and NAT10 expression?",{"text":116,"@type":112},"ac4C modification levels were assessed using a dot blotting assay, while NAT10 levels were measured by RT-qPCR.",{"name":118,"@type":109,"acceptedAnswer":119},"What mechanism connects NAT10 to SERPINA1 mRNA?",{"text":120,"@type":112},"NAT10 directly binds to SERPINA1 mRNA and enhances its ac4C modification, increasing SERPINA1 mRNA stability in an ac4C-dependent manner.","https://schema.org",{"og:url":83,"og:type":123,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":125,"canonical":83},"index,follow",{"doc_id":127,"site_id":62},363962,1790194449,{"code":4,"msg":5,"data":130},{"doc_id":127,"user_id":131,"nickname":92,"user_avatar":132,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":133,"file_id":134,"file_url":135,"file_type":136,"file_size":137,"view_count":14,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":138,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144},962090880963,"https://ap-avatar.wpscdn.com/davatar_6f874abed73319feea01a86fa6f0fab8","Fan and Ma BMC Cancer (2026) 26:917 BMC Cancer  \n[https://doi.org/10.1186/s12885-026-16215-8](https://doi.org/10.1186/s12885-026-16215-8)  \nRESEARCH Open Access  \nAcetyltransferase NAT10 promotes aggressiveness of colorectal cancer cells via ac4 C acetylation of SERPINA1 mRNA  \nWeicong Fan 1 and Xinlei Ma2*  \nAbstract  \nBackground N-acetyltransferase 10 (NAT10) mediated N4-acetylcytidine (ac4C) modification is a significant RNA modification in various tumors. This study aimed to investigate the underlying mechanism of NAT10 modulating the progression of colorectal cancer via mRNA ac4C modification.  \nMethods Aberrant expressed NAT10 in colorectal cancer was identified from GSE37182 dataset. The ac4C modification levels in colorectal cancer cells were accessed by dot blotting assay, and the NAT10 levels were accessed by RT-qPCR. Cell viability, proliferation, invasion, and migration of colorectal cancer cells were evaluated to determine the regulatory role of NAT10 on cell aggressiveness. The interaction between NAT10 and Serine protease inhibitor A1 (SERPINA1) was verified using RNA immunoprecipitation (RIP) and ac4C RNA immunoprecipitation (ac4C RIP), and Luciferase reporter assay. Moreover, the regulatory role of NAT10 on tumorigenesis of colorectal cancer cells in vivo was investiaged in xenograft tumor model.  \nResults Global ac4C modification and NAT10 expression were significantly elevated in colorectal cancer cells.. Downregulation of NAT10 inhibited cell proliferation, invasion, and migration of colorectal cancer cells. Mechanistically, NAT10 bound directly to SERPINA1 mRNA and enhanced its ac4C modification, thereby increasing its stability. This regulation was proven to be ac4C-dependent. Over-expression of SERPINA1 partially weakened the suppression effects of NAT10 knockdown on aggressiveness of colorectal cancer cells. Furthermore, pharmacological inhibition of NAT10 significantly suppressed tumor growth in vivo.  \nConclusion Our findings demonstrate that NAT10 promotes the aggressiveness of colorectal cancer cells by regulating the ac4C modification of SERPINA1 mRNA, highlighting this axis as a potential therapeutic target. Keywords Colorectal cancer, NAT10, SERPINA1, Ac4C  \n*Correspondence:  \nXinlei Ma  \n[fxwcong2025@163.com](fxwcong2025@163.com)  \n1Department of Colorectal Medicine, Dongguan Traditional Chinese Medicine Hospital, Dongguan, China  \n2Department of Spleen and Stomach Diseases, Dongguan Traditional Chinese Medicine Hospital, No. 3, Dongcheng Section, Songshan Lake Avenue, Dongcheng District, Dongguan City,  \nGuangdong Province 523000, China  \n© The Author(s) 2026. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit [http://creati](http://creati)[vecommons.org/licenses/by-nc-nd/4.0/](vecommons.org/licenses/by-nc-nd/4.0/.)[.](vecommons.org/licenses/by-nc-nd/4.0/.)  \nIntroduction  \nColorectal cancer remains a leading cause of global cancer-related morbidity and mortality. A significant proportion of patients are diagnosed at advanced stages, presenting with malignant features such as local invasion ","cbCaitiXOKtBMdCt","https://ap.wps.com/l/cbCaitiXOKtBMdCt","pdf",9714346,13,"English","# Abstract\n## Background\n## Methods\n## Results\n## Conclusion\n# Introduction","[{\"question\":\"What role does NAT10 play in colorectal cancer according to the study?\",\"answer\":\"NAT10 promotes colorectal cancer aggressiveness by regulating ac4C modification of SERPINA1 mRNA. Increased NAT10 and global ac4C levels were observed in colorectal cancer cells.\"},{\"question\":\"How did the study measure ac4C modification and NAT10 expression?\",\"answer\":\"ac4C modification levels were assessed using a dot blotting assay, while NAT10 levels were measured by RT-qPCR.\"},{\"question\":\"What mechanism connects NAT10 to SERPINA1 mRNA?\",\"answer\":\"NAT10 directly binds to SERPINA1 mRNA and enhances its ac4C modification, increasing SERPINA1 mRNA stability in an ac4C-dependent manner.\"}]","Acetyltransferase NAT10 promotes aggressiveness of colorectal cancer cells via ac4C acetylation of SERPINA1 mRNA | PDF",1790154523,33]