[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-289638-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-289638-en":130},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","a-study-of-clinical-and-serological-correlation-of-positive-direct-antiglobulin-test-in-blood-bank-at-a-tertiary-care-center","A Study of Clinical and Serological Correlation of Positive Direct Antiglobulin Test in Blood Bank at a Tertiary Care Center","","Prospective observational study evaluated clinical and serological correlations of positive direct antiglobulin test (DAT) in a tertiary care blood bank. Two hundred DAT-positive samples (polyspecific baseline) were tested with monospecific anti-IgG and anti-C3 reagents using tube techniques, with DAT graded from 1+ to 4+ and autocontrol included. Patients were categorized by clinical conditions, and in vivo hemolysis was assessed across categories and DAT grades. Incidence of in vivo hemolysis increased significantly with higher DAT grades; autoimmune diseases predominated. Only anti-C3 positivity showed no hemolysis.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/a-study-of-clinical-and-serological-correlation-of-positive-direct-antiglobulin-test-in-blood-bank-at-a-tertiary-care-center/289638/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/a-study-of-clinical-and-serological-correlation-of-positive-direct-antiglobulin-test-in-blood-bank-at-a-tertiary-care-center/289638.png","ImageObject",300,407,{"name":42,"@type":43},"Paura","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-21","2026-09-17",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",9,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What was the study’s main objective regarding positive DAT?","Question",{"text":63,"@type":64},"To evaluate clinical and serological correlation of positive DAT by categorizing clinical conditions, assessing in vivo hemolysis for positive DAT, and correlating DAT strength with hemolysis.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How were DAT-positive samples tested in the study?",{"text":68,"@type":64},"Two hundred samples positive by polyspecific antiglobulin reagent were retested with anti-IgG and anti-C3 monospecific DAT reagents. Tube technique was used, with DAT positivity graded 1+ to 4+ and autocontrol included.",{"name":70,"@type":61,"acceptedAnswer":71},"What did the results show about the relationship between DAT grade and hemolysis?",{"text":72,"@type":64},"The incidence of in vivo hemolysis increased significantly with higher grades of DAT positivity across the antihuman globulin reagents.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},289638,1789636126,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,110,115,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":108,"slug":109},7,40,"healthcare",{"id":111,"doc_module":4,"doc_module_name":25,"category_name":112,"show_sort_weight":113,"slug":114},8,"Research & Report",30,"research-report",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":97,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":107,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":111,"language":139,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":12,"update_tm":80,"read_time":117},13056712833777,"https://ap-avatar.wpscdn.com/davatar_29158cc5080c5b710cf443261637dec0","Published online: 2022-01-18  \nOriginal Article  \nA Study of Clinical and Serological Correlation of Positive Direct AntiglobulinTest in Blood Bank ata Tertiary Care Center  \nAlisha Suresh Kerkar 1 Swarupa Nikhil Bhagwat 1 Jayashree Harihara Sharma 1  \n1 Department of Transfusion Medicine, Seth GS Medical College and KEM Hospital, Mumbai, Maharashtra, India  \nJ Lab Physicians  \nAddress for correspondence Swarupa Nikhil Bhagwat, MD, Department of Transfusion Medicine, Seth GS Medical College and KEM Hospital, Mumbai 400012, India  \n(e-mail: [swarupabhagwat@kem.edu](swarupabhagwat@kem.edu)).  \nAbstract  \nKeywords  \n► direct antiglobulin test  \n► in vivo hemolysis  \n► autoimmune hemolytic anemia  \n► connective tissue disorders  \nObjectives Detection of red cell bound immunoglobulins and/or complement by direct antiglobulin test (DAT) is a crucial serological assay in the diagnosis of autoimmune hemolytic anemia (AIHA) . However, DAT may be positive in a variety of clinical conditions with or without hemolysis. We aimed at evaluating the clinical and serological correlation of positive DAT by categorizing the clinical conditions associatedwith positive DAT, estimating the presence of in vivo hemolysis incase of positive DATwith polyspecific and monospecificantisera and correlating the strength of positive DAT with the presence of hemolysis. Materials and Methods The prospective observational study was performed on 200 samples that were positive for DAT with polyspecific antiglobulin reagent as the baseline investigation. These samples were further tested with anti-immunoglobulin G and anti-C3 monospecific DAT reagents to evaluate the type of protein responsible for positive DAT. The antiglobulin tests were performed by tube technique. DAT positivity was graded (1þ to 4 þ ) in each patient. Autocontrol test was included. The patients with positive polyspecific DAT were categorized into different clinical conditions. The presence or absence of in vivo hemolysis was evaluated in all clinical categories and also for each grade of positivity with polyspecific and monospecific antiglobulin reagents. Statistical Analysis Binomial logistic regression and Mann–Whitney U test were applied to between the group analyses. For categorical variables, Fisher’sexact test and relative risk were used. The qualitative data were expressed in numbers and percentages.  \nResults The highest number of patients (75/200, 37.5%) belonged to the autoimmune diseases group. Tuberculosis and hepatitis C were the main infectious diseases associated with positive DAT. Out of200DAT-positive patients,98(49%)had in vivo hemolysis and102 (51%) did not have hemolysis. AIHA (22) and systemic lupus erythematosus (18) were the commonest clinical conditions associated with in vivo hemolysis. All the 11 samples that showed positivity with only anti-C3 reagent did not show any hemolysis. There was statistically significant increase in the incidence of in vivo hemolysis with increasing grades of DAT positivity with all the three antihuman globulin reagents.  \nConclusion There are different disease conditionswhich show positive DATwith or without hemolysis. So, it is important to clinically and serologically correlate positive DAT results.  \nDOI [https://doi.org/](https://doi.org/)[ ](https://doi.org/)10.1055/s-0041-1741442 . ISSN 0974-2727.  \n© 2022 . The Indian Association of Laboratory Physicians. All rights reserved.  \nThis is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. ([https://creativecommons.org/](https://creativecommons.org/)[ ](https://creativecommons.org/)[licenses/by-nc-nd/4.0/](licenses/by-nc-nd/4.0/))  \nThieme Medical and Scientific Publishers Pvt. Ltd. , A-12, 2nd Floor, Sector 2, Noida-201301 UP","cbCaia4spTudL2ZO","https://ap.wps.com/l/cbCaia4spTudL2ZO","pdf",171110,"English","# Objectives\n# Materials and Methods\n# Results\n# Conclusion\n# Introduction","[{\"question\":\"What was the study’s main objective regarding positive DAT?\",\"answer\":\"To evaluate clinical and serological correlation of positive DAT by categorizing clinical conditions, assessing in vivo hemolysis for positive DAT, and correlating DAT strength with hemolysis.\"},{\"question\":\"How were DAT-positive samples tested in the study?\",\"answer\":\"Two hundred samples positive by polyspecific antiglobulin reagent were retested with anti-IgG and anti-C3 monospecific DAT reagents. Tube technique was used, with DAT positivity graded 1+ to 4+ and autocontrol included.\"},{\"question\":\"What did the results show about the relationship between DAT grade and hemolysis?\",\"answer\":\"The incidence of in vivo hemolysis increased significantly with higher grades of DAT positivity across the antihuman globulin reagents.\"}]","A Study of Clinical and Serological Correlation of Positive Direct Antiglobulin Test in Blood Bank at a Tertiary Care Center | PDF"]