[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-446508-105":59,"doc-detail-446508-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","a-simple-and-standardized-method-supports-efficient-derivation-of-clinical-grade-human-embryonic-stem-cells-under-feeder-and-xenofree-conditions","A simple and standardized method supports efficient derivation of clinical-grade human embryonic stem cells under feeder-and xenofree conditions","","Human embryonic stem cells (hESCs) are valuable for replacement therapies, yet clinically compliant hESC line generation under feeder-free and xeno-free conditions remains inefficient. This work develops and tests a standardized, translation-ready culture approach to derive hESCs from clinically surplus, discarded low-quality frozen-thawed embryos. By optimizing culture and inner cell mass isolation, derivation efficiency improves markedly, yielding 16 lines, including two clinical-grade lines meeting GMP criteria.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/a-simple-and-standardized-method-supports-efficient-derivation-of-clinical-grade-human-embryonic-stem-cells-under-feeder-and-xenofree-conditions/446508/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/a-simple-and-standardized-method-supports-efficient-derivation-of-clinical-grade-human-embryonic-stem-cells-under-feeder-and-xenofree-conditions/446508.png","ImageObject",300,407,{"name":92,"@type":93},"Stanford","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-30","2026-09-29",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"Why is deriving clinically compliant hESC lines under feeder-free and xeno-free conditions important?","Question",{"text":112,"@type":113},"Clinically compliant hESC lines are needed for reliable translation to clinical settings. Feeder- and xeno-free conditions provide a more consistent and standardized culture environment, supporting reproducibility essential for clinical application.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What method is proposed to improve hESC derivation efficiency?",{"text":117,"@type":113},"The study optimizes culture conditions and the inner cell mass (ICM) isolation approach to enable efficient hESC derivation from clinically surplus, discarded frozen-thawed embryos under feeder-and xeno-free conditions.",{"name":119,"@type":110,"acceptedAnswer":120},"How do derivation efficiencies vary with ICM quality in this protocol?",{"text":121,"@type":113},"For blastocysts with ICM grades A and B, derivation efficiency reaches about 60%, with 100% derivation for grade A ICMs. For grade C embryos with poorly developed ICMs, efficiency approaches about 30%, demonstrating robustness across ICM quality.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},446508,1791204400,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},2336477552062,"https://ap-avatar.wpscdn.com/davatar_994ba38a5ba835b3df7d355c54d3ed8d","Zhang et al. Stem Cell Research & Therapy (2026) 17:9 [https://doi.org/10.1186/s13287-025-04831-3](https://doi.org/10.1186/s13287-025-04831-3)  \nStem Cell Research & Therapy  \nRESEARCH Open Access  \nA simple and standardized method supports  efficient derivation of clinical-grade human embryonic stem cells under feeder-and xenofree conditions  \nQiong Zhang 1†, Huicheng Chen 1†, Jintao Cun2†, Qianqian Liu3†, Hao Wang4, Luxi Jiang 1, Shubo Hu5, Hongkun Wang3, Jingxin Wang3, Jian Xu 1, Jinyue Shi 1, Fangyuan Sun2, Xianlang Xiong6, Zhen Liang6*, Yuansong Yu5* and  \nYuanyuan Du3*  \nAbstract  \nBackground Human embryonic stem cells (hESCs), as naturally pl uripotent stem cells, constitute a pivotal cell source for cell replacement therapies. Yet, the generation of clinically compliant hESC lines under feeder-free and xeno-free conditions remains inefficient. Moreover, the derivation of hESCs from clinically surplus and discarded low-quality embryos using the standardized, translation-ready culture systems has not been reported.  \nMethods By optimizing culture conditions and inner cell mass (ICM) isolation method, we developed a method that significantly improves the derivation efficiency of hESC lines from clinically surplus and discarded frozenthawed embryos under feeder-and xeno-free conditions. The derivation protocol is operationally simple and easily standardized, with the reagents commercially available and of GMP-grade.  \nResults Using this protocol, we successfully established 16 hESC lines. Among blastocysts with morphologically distinct ICMs of grades A and B, the derivation efficiency achieved approximately 60%, with all three grade A ICMs yielding viable hESC lines (100% derivation efficiency for grade A) . Notably, for embryos with poorly developed ICMs (grade C), the derivation efficiency of hESC lines approached 30%, showing the protocol’s robustness across varying ICM quality. Adhering to GMP standards, we derived two clinical-grade hESC lines, which were demonstrated biological safety, sustained pl uripotency, and the capacity for three-germ-layer differentiation.  \n†Qiong Zhang, Huicheng Chen, Jintao Cun and Qianqian Liu have contributed equally to this work.  \n*Correspondence:  \nZhen Liang [liangzhenzzu@163.com](liangzhenzzu@163.com)[ ](liangzhenzzu@163.com)Yuansong Yu[yuyuansong@eetop.com](yuyuansong@eetop.com)[ ](yuyuansong@eetop.com)Yuanyuan Du[duyuanyuan@him.cas.cn](duyuanyuan@him.cas.cn)  \nFull list of author information is available at the end of the article  \n© The Author(s) 2025. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit [http://creativecommons.org/l](http://creativecommons.org/l)icenses/by/4.0/.  \nZhang et al. Stem Cell Research & Therapy (2026) 17:9 Page 2 of 13  \nConclusions Our study offers a robust, standardized, and simple method for deriving clinical-grade hESCs. Efficient derivation, propagation and banking of hESC lines from frozen-thawed embryos would offer a valuable cell source for advancing regenerative medicine, disease modeling, and drug development.  \nKeywords Human embryonic stem cells, Clinical-grade, Derivation under feeder-and xeno-free conditions  \nBackground  \nHuman pluripotent stem cells (hPSCs) can self-renew indefinitely and differen","cbCaivyQtPD6PUry","https://ap.wps.com/l/cbCaivyQtPD6PUry","pdf",8809481,13,"English","# Abstract\n## Background\n## Methods\n## Results\n## Conclusions\n## Keywords\n# Background\n## Prior feeder-based approaches\n## Need for standardized xeno-free derivation\n# Protocol overview\n## Derivation efficiency across ICM grades","[{\"question\":\"Why is deriving clinically compliant hESC lines under feeder-free and xeno-free conditions important?\",\"answer\":\"Clinically compliant hESC lines are needed for reliable translation to clinical settings. Feeder- and xeno-free conditions provide a more consistent and standardized culture environment, supporting reproducibility essential for clinical application.\"},{\"question\":\"What method is proposed to improve hESC derivation efficiency?\",\"answer\":\"The study optimizes culture conditions and the inner cell mass (ICM) isolation approach to enable efficient hESC derivation from clinically surplus, discarded frozen-thawed embryos under feeder-and xeno-free conditions.\"},{\"question\":\"How do derivation efficiencies vary with ICM quality in this protocol?\",\"answer\":\"For blastocysts with ICM grades A and B, derivation efficiency reaches about 60%, with 100% derivation for grade A ICMs. For grade C embryos with poorly developed ICMs, efficiency approaches about 30%, demonstrating robustness across ICM quality.\"}]","A simple and standardized method supports efficient derivation of clinical-grade human embryonic stem cells under feeder-and xenofree conditions | PDF",1790715683,33]