[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-408405-105":59,"doc-detail-408405-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","a-pilot-study-for-decellularizing-porcine-cornea-for-future-use-in-corneal-regeneration","A pilot study for decellularizing porcine cornea for future use in corneal regeneration","","Porcine corneas were decellularized for future use in corneal regeneration using washing steps with the CHAPS detergent, Benzonase, and EDTA. Decellularization quality was evaluated by quantitative and qualitative DNA measurements, assessment of glycosaminoglycans (GAGs), immunofluorescent staining for extracellular matrix proteins (including collagens and keratocyte-associated markers), and transmission electron microscopy to examine collagen fibril structure. The method achieved 99.5% DNA reduction while preserving GAGs, removing nuclei, and maintaining collagen fibril patterns comparable to native tissue.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/a-pilot-study-for-decellularizing-porcine-cornea-for-future-use-in-corneal-regeneration/408405/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/a-pilot-study-for-decellularizing-porcine-cornea-for-future-use-in-corneal-regeneration/408405.png","ImageObject",300,407,{"name":92,"@type":93},"jayni","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-30","2026-09-28",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What agents were used to decellularize the porneal tissue?","Question",{"text":112,"@type":113},"The porcine corneas were decellularized using washing steps with CHAPS detergent, Benzonase, and EDTA.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was decellularization effectiveness assessed?",{"text":117,"@type":113},"Effectiveness was assessed using DNA content measurements, GAG assessment, immunofluorescent staining for matrix proteins, and transmission electron microscopy of collagen fibrils.",{"name":119,"@type":110,"acceptedAnswer":120},"What were the main results regarding DNA and extracellular matrix preservation?",{"text":121,"@type":113},"The protocol reduced DNA content by 99.5% and preserved GAGs to an acceptable extent while maintaining collagen fibril structure and associated proteins, with no cell nuclei detected by staining.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},408405,1790719371,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},3985747859343,"https://ap-avatar.wpscdn.com/davatar_276721f389ce27ea32af1340a28f341c","OPEN ACCESS  \nCitation: Josifovska N, Niemi EM, Ramalingam M, Manivasagam G, Scholz H, Petrovski G (2025) A pilot study for decellularizing porcine cornea for future use in corneal regeneration. PLoSOne 20(12): e0339462. [https://doi.org/10.1371/](https://doi.org/10.1371/)[ ](https://doi.org/10.1371/)[journal.pone.0339462](journal.pone.0339462)  \nEditor: Panayiotis Maghsoudlou, University College London Institute of Child Health, UNITED KINGDOM OF GREAT BRITAIN AND NORTHERN IRELAND  \nReceived: September 17, 2025  \nAccepted: December 8, 2025  \nPublished: December 31, 2025  \nCopyright: © 2025 Josifovska et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.  \nData availability statement: All relevant data are within the manuscript and its Supporting  Information files.  \nFunding: This research was supported by the Norwegian Research Council (Grant No.  \nRESEARCH ARTICLE  \nA pilot study for decellularizing porcine cornea for future use in corneal regeneration  \nNatasha Josifovska1☯, Essi M. Niemi2,3☯, Murugan Ramalingam4, 5, 6, 7, 8, 9, Geetha Manivasagam4, Hanne Scholz3,10, Goran Petrovski1, 11, 12*  \n1 Center for Eye Research and Innovative Diagnostics, Department of Ophthalmology, Oslo University Hospital, and Institute for Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway,  \n2 Vascular Biology and Surgery Group, Institute for Surgical Research and Department of Vascular Surgery, Oslo University Hospital, Oslo, Norway, 3 Hybrid Technology Hub, Centre of Excellence, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway, 4 Centre for Biomaterials, Cellular and Molecular Theranostics, Vellore Institute of Technology, Vellore, Tamil Nadu, India, 5 NanoBioCel Group, Department of Pharmacy and Food Sciences, Faculty of Pharmacy, University of the Basque Country (UPV/EHU),Vitoria-Gasteiz, Spain, 6 Bioaraba Health Research Institute, Jose Atxotegi, s/n, Vitoria-Gasteiz, Spain, 7 Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Institute of Health Carlos III, Madrid, Spain, 8 Joint Research Laboratory (JRL) on Bioprinting and Advanced Pharma Development, A Joint Venture of TECNALIA (Basque Research and Technology Alliance), Lascaray Research Center, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain, 9 IKERBASQUE, Basque Foundation for Science, Bilbao, Spain, 10 Institute for Surgical Research and Section for Transplant Surgery, Cell Transplantation and Tissue Engineering Group, Oslo University Hospital, Oslo, Norway, 11 Department of Ophthalmology, University Hospital Split; School of Medicine, University of Split, Split, Croatia, 12 UKLONetwork, University St. Kliment Ohridski, Bitola, North Macedonia  \n☯ These authors contributed equally to this work.  \n* [goran.petrovski@medisin.uio.no](goran.petrovski@medisin.uio.no)  \nAbstract  \nPorcine corneas were decellularized for future use in corneal regeneration by  \nusing various washing steps with 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) detergent, Benzonase, and Ethylenediaminetetraacetic acid (EDTA) . The quality of the decellularized corneas was assessed by quantitative and qualitative measurement of DNA content, glycosaminoglycans (GAGs), immunofluorescent staining for Collagen (Col) I, V, Keratocan, Fibronectin, Laminin, Lumicanand Decorin, and Transmission Electron Microscopy (TEM) to observe the structure of the collagen fibrils was used. The decellularization process showed 99.5% DNA content reduction in the corneas and a similar pattern was observed in the preservation of GAGs. Hematoxylin & Eosin (H&E) and immunofluorescent staining showed no presence of cell nuclei, while Alcian blue staining confirmed the presence of GAGs. Col I, V, Keratocan, Fibronectin, Lamin","cbCaitDozgK0cqJH","https://ap.wps.com/l/cbCaitDozgK0cqJH","pdf",2073935,15,"English","# Abstract\n# Introduction","[{\"question\":\"What agents were used to decellularize the porneal tissue?\",\"answer\":\"The porcine corneas were decellularized using washing steps with CHAPS detergent, Benzonase, and EDTA.\"},{\"question\":\"How was decellularization effectiveness assessed?\",\"answer\":\"Effectiveness was assessed using DNA content measurements, GAG assessment, immunofluorescent staining for matrix proteins, and transmission electron microscopy of collagen fibrils.\"},{\"question\":\"What were the main results regarding DNA and extracellular matrix preservation?\",\"answer\":\"The protocol reduced DNA content by 99.5% and preserved GAGs to an acceptable extent while maintaining collagen fibril structure and associated proteins, with no cell nuclei detected by staining.\"}]","A pilot study for decellularizing porcine cornea for future use in corneal regeneration | PDF",1790579284,38]