[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-333643-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-333643-en":130},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","a-new-evaluation-of-the-rearranged-noncoding-control-region-of-jc-virus-in-patients-with-colorectal-cancer-research-findings","A new evaluation of the rearranged noncoding control region of JC virus in patients with colorectal cancer - Research Findings","","A case-control evaluation assesses rearranged noncoding control region (NCCR) variants of JC virus (JCV) in colorectal cancer (CRC). The study compares tumor tissues (n=60), adjacent normal tissues (n=60), and urine samples (n=60), detecting VP1 and NCCR by nested PCR, followed by sequencing and phylogenetic genotyping. JCV LTAg expression is examined in tissues using GraphPad Prism. JCV DNA is detected in tumor, normal, and urine specimens, with genotype 3 predominance and distinct rrNCCR block patterns, while LTAg expression differs significantly between tumor and normal tissue.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & Report",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/a-new-evaluation-of-the-rearranged-noncoding-control-region-of-jc-virus-in-patients-with-colorectal-cancer-research-findings/333643/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/a-new-evaluation-of-the-rearranged-noncoding-control-region-of-jc-virus-in-patients-with-colorectal-cancer-research-findings/333643.png","ImageObject",300,407,{"name":42,"@type":43},"Liam","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-26","2026-09-21",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",8,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What was the main goal of evaluating JCV in colorectal cancer patients?","Question",{"text":63,"@type":64},"To assess the rearranged NCCR region of detected JCV DNA in CRC patient tissue samples and examine associated genotypes and expression patterns.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How were JCV DNA and genotypes determined in the study?",{"text":68,"@type":64},"Nested PCR detected VP1 and NCCR regions, positive products were sequenced, and a phylogenetic tree was constructed to determine JCV genotypes.",{"name":70,"@type":61,"acceptedAnswer":71},"What relationship did the study find between JCV LTAg expression and tumor versus normal tissue?",{"text":72,"@type":64},"JCV LTAg expression was significantly different in tumor tissue compared with adjacent normal tissue, indicating a potential latent JCV status during cancer development.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},333643,1790160314,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,111,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":108,"show_sort_weight":109,"slug":110},7,"Healthcare",40,"healthcare",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":112,"slug":113},30,"research-report",{"id":115,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},9,"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":97,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":55,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":139,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":145,"preview_page_count":4},8796095461564,"https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d","Haghi Navand etal. BMC Cancer (2024) 24:1001 BMC Cancer  \n[https://doi.org/10.1186/s12885-024-12684-x](https://doi.org/10.1186/s12885-024-12684-x)  \nRESEARCH Open Access  \nA new evaluation of the rearranged noncoding control region of JC virus in patients with colorectal cancer  \nAzadeh Haghi Navand 1, Shahram Jalilian 1, Kambiz Ahmadi Angali2, Mohammad Karimi Babaahmadi3, Abdol hassanTalaiezadeh 1 and Manoochehr Makvandi 1*  \nAbstract  \nBackground Several studies have reported the presence of JC virus (JCV) in human tumors, The association of JCVand CRC remains controversial. This study aimed to evaluate the rearranged NCCR region of the detected JCV DNA in CRC patients’ tissue samples.  \nMethods In this case-control study, tumor tissues (n = 60), adjacent normal tissues (n = 60), and urine samples (n = 60) of the CRC patients were collected. The nested PCR was employed to detect the VP1 and NCCR regions of the JCV genome. The positive JCV PCR products were sequenced and a phylogenetic tree was constructed to determine the JCV genotypes. After extracting RNA and preparing cDNA, the expression of JCV LTAg was examined in 60 tumor tissues and 60 adjacent normal tissues. The analysis of JCV LTAg expression was performed using GraphPad Prism software version 8.  \nResults The analysis reveals that JCV DNA was detected in 35/60 (58 . 3%) tumor tissues, while 36/60 (60 . 0%) of adjacent normal tissues (p = 0 . 85) . JCV DNA was detected in 42/60 (70 . 0%) urine samples when compared to 35/60 (58 . 3%) tumor tissues of CRC patients and was not found significant (P = 0 . 25) . The phylogenetic tree analysis showed the dominant JCV genotype 3, followed by genotype 2D was distributed in tumor tissue, normal tissue, and urine samples ofthe CRC patients. Analysis of randomly selected NCCR sequences from JCV regions in tumor tissue samples revealed the presence of rearranged NCCR blocks of different lengths.: 431 bp, 292 bp, 449 bp, and 356 bp. These rearranged NCCR blocks differ from the rearranged NCCR blocks described in PML-type Mad-1, Mad-4, Mad-7, and Mad-8 prototypes. The expression of JCV LTAg was significantly different in tumor tissue compared to normal tissue, with a p-value of less than 0.002.  \nConclusion A significant proportion of 35%> of the tumor tissue and urine samples ofthe CRC patients was found to be positive for JCV DNA (P = 0 . 25) . The parallel analysis of tumor and urine samples for JCV DNA further supports the potential for non-invasive screening tools. This study provides new insights into Rearranged NCCR variant isolates from patients with CRC. The significant difference in JCV LTAg expression between tumor and normal tissue indicates a latent JCV status potentially leading to cancer development.  \n*Correspondence:  \nManoochehr Makvandi[manoochehrmakvandi299@gmail.com](manoochehrmakvandi299@gmail.com)  \nFull list of author information is available at the end of the article  \n© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit [http://](http://)[ ](http://)[creativecommons.org/l](creativ","cbCailm1DP7GlF2b","https://ap.wps.com/l/cbCailm1DP7GlF2b","pdf",1391445,11,"English","# Abstract\n## Background\n## Methods\n## Results\n## Conclusion\n# Keywords\n## Background\n## Epidemiology and risk factors\n## JCV acquisition and relevance","[{\"question\":\"What was the main goal of evaluating JCV in colorectal cancer patients?\",\"answer\":\"To assess the rearranged NCCR region of detected JCV DNA in CRC patient tissue samples and examine associated genotypes and expression patterns.\"},{\"question\":\"How were JCV DNA and genotypes determined in the study?\",\"answer\":\"Nested PCR detected VP1 and NCCR regions, positive products were sequenced, and a phylogenetic tree was constructed to determine JCV genotypes.\"},{\"question\":\"What relationship did the study find between JCV LTAg expression and tumor versus normal tissue?\",\"answer\":\"JCV LTAg expression was significantly different in tumor tissue compared with adjacent normal tissue, indicating a potential latent JCV status during cancer development.\"}]","A new evaluation of the rearranged noncoding control region of JC virus in patients with colorectal cancer - Research Findings | PDF",1790003429,28]