[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-374247-105":59,"doc-detail-374247-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","a-mechanistic-study-of-the-feasibility-of-ursodeoxycholic-acid-in-the-treatment-of-colon-adenocarcinoma","A Mechanistic Study of the Feasibility of Ursodeoxycholic Acid in the Treatment of Colon Adenocarcinoma","","Ursodeoxycholic acid (UDCA) is recognized as a bile-acid–related drug, yet its mechanistic role in colon adenocarcinoma remains unclear. This study evaluates the connection between colon adenocarcinoma and bile-acid metabolism and tests the therapeutic feasibility of UDCA. Differential expression and WGCNA from TCGA identify COAD core genes, followed by GO/KEGG pathway enrichment. Target intersection, molecular docking, and in vitro assays (MTT, wound healing, transwell migration, angiogenesis) assess anti-tumor effects.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/a-mechanistic-study-of-the-feasibility-of-ursodeoxycholic-acid-in-the-treatment-of-colon-adenocarcinoma/374247/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/a-mechanistic-study-of-the-feasibility-of-ursodeoxycholic-acid-in-the-treatment-of-colon-adenocarcinoma/374247.png","ImageObject",300,407,{"name":92,"@type":93},"Theodore","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-28","2026-09-23",true,{"@type":102,"interactionType":103,"userInteractionCount":44},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the primary research goal of this study?","Question",{"text":112,"@type":113},"To investigate the relationship between colon adenocarcinoma and bile-acid metabolism and to assess the feasibility of ursodeoxycholic acid for colon adenocarcinoma treatment.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were COAD-related targets and core genes identified?",{"text":117,"@type":113},"The study used TCGA-based differential expression and weighted gene co-expression network analysis (WGCNA), followed by GO annotation and KEGG pathway enrichment.",{"name":119,"@type":110,"acceptedAnswer":120},"What evidence supports UDCA’s potential anti-tumor activity?",{"text":121,"@type":113},"Database-based target intersection identified UDCA anti-tumor targets, STRING/Cytoscape selected core targets, molecular docking showed good binding, and in vitro assays demonstrated inhibition of proliferation, migration, invasion, and neovascularization.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},374247,1790243250,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":44,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},7971461740886,"https://ap-avatar.wpscdn.com/davatar_3d24733baf745e90a7e4bdd5f77d97b2","Drug Design, Development and Therapy  \nOpen Access Full Text Article ORIGINAL RESEARCH  \nA Mechanistic Study of the Feasibility of Ursodeoxycholic Acid in the Treatment of Colon Adenocarcinoma  \nShuyu Liu 1 , 2 , *, Mengyue Zhou 3 , *, Xiaoli Huang2 , *, Peng Chen2 , Quanpeng Li 1 , Yuting Wang 1 , Xianxiu Ge 1 , Fei Wang 1 , Jianing Xu 1 , Jiayi Gu4 , Lin Miao 1 , Xueting Deng 1  \n1Medical Center for Digestive Disease, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, People’s Republic of China; 2Department of Gastroenterology, The Fourth Affiliated Hospital of Nanjing Medical University, Nanjing, People’s Republic of China; 3Department of Gastroenterology, Nanjing Pukou Hospital of Traditional Chinese Medicine, Nanjing, People’s Republic of China; 4Department of Neurology, The Fourth Affiliated Hospital of Nanjing Medical University, Nanjing, People’s Republic of China  \n*These authors contributed equally to this work  \nCorrespondence: Lin Miao; Xueting Deng, Medical Center for Digestive Disease, The Second Affiliated Hospital of Nanjing Medical University, 121 Jiangjiayuan, Nanjing, Jiangsu, 210011, People’s Republic of China, Tel +86-13913862526, Email [linmiao@njmu.edu.cn](linmiao@njmu.edu.cn); [2926325679@qq.com](2926325679@qq.com)  \n\n| Purpose: Bile acids promote the progression of colon adenocarcinoma (COAD), and ursodeoxycholic acid (UDCA) is a key drug in promoting bile acid excretion, but its role in COAD unclear. Our study aims to investigate the relationship between COAD and bile acid metabolism and to assess the feasibility of UDCA for the treatment of COAD.\u003Cbr>Methods: Firstly, biological targets closely related to COAD were identified: Based on the cancer genome atlas (TCGA) database, the core genes of COAD were obtained by differential expression analysis and weighted gene-coexpression network analysis (WGCNA), and subjected to gene ontology (GO) function annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Secondly, finding a drug by target, after identifying UDCA as a candidate drug, the feasibility of UDCA in treating COAD was verified in reverse: Using databases to collect potential targets for COAD and UDCA, and the intersecting genes were the potential targets for UDCA to exert anti-tumor effects. Then Autodock was used for molecular docking to analyze the interaction between UDCA and core target proteins. Finally, experimental validation was performed: MTT assay, wound healing, transwell migration, and angiogenesis assays were used to detect the effects of UDCA on cell proliferation, migration, invasion, and neovascularization.\u003Cbr>Results: 2064 differential genes were screened from TCGA. WGCNA obtained the module most relevant to CRC, containing 493 genes. KEGG analysis found that overlapping genes were mainly concentrated in bile acid metabolic pathways. A total of 26 UDCA anti-tumor targets were obtained in database, and 5 core targets were selected by STRING database and Cytoscape software: TNF, CYP27B1, MDM2, MMP2, CASP3 . Molecular docking results showed that UDCA had good binding activity with the core targets. In vitro experiment showed UDCA effectively inhibited the proliferation, migration, invasion and neovascularization in colon cancer cells. Conclusion: The antitumor activity of ursodeoxycholic acid may be related to cell apoptosis, proliferation, migration and vascular neogenesis.\u003Cbr>Keywords: ursodeoxycholic acid, colon adenocarcinoma treatment, bile acid metabolism, cell death |\n| --- |\n| Background\u003Cbr>Colon adenocarcinoma is at the forefront of the incidence and mortality of malignant tumors. According to the 2023 Global Cancer Data report, the incidence of colon adenocarcinoma ranks the third among malignant tumors (10%) and the mortality ranks the second among malignant tumors (9 .4%), 1 ,2 which seriously endangers public life and health. The pathogenesis o f colon adenocarcinoma is complex, and the only way to treat the disea","cbCaisxZ7JHRvOTB","https://ap.wps.com/l/cbCaisxZ7JHRvOTB","pdf",8507714,14,"English","# Purpose\n# Methods\n## Target identification and drug-target selection\n## Molecular docking and experimental validation\n# Results\n# Conclusion\n# Background\n# Publication information","[{\"question\":\"What is the primary research goal of this study?\",\"answer\":\"To investigate the relationship between colon adenocarcinoma and bile-acid metabolism and to assess the feasibility of ursodeoxycholic acid for colon adenocarcinoma treatment.\"},{\"question\":\"How were COAD-related targets and core genes identified?\",\"answer\":\"The study used TCGA-based differential expression and weighted gene co-expression network analysis (WGCNA), followed by GO annotation and KEGG pathway enrichment.\"},{\"question\":\"What evidence supports UDCA’s potential anti-tumor activity?\",\"answer\":\"Database-based target intersection identified UDCA anti-tumor targets, STRING/Cytoscape selected core targets, molecular docking showed good binding, and in vitro assays demonstrated inhibition of proliferation, migration, invasion, and neovascularization.\"}]","A Mechanistic Study of the Feasibility of Ursodeoxycholic Acid in the Treatment of Colon Adenocarcinoma | PDF",1790201355,35]