[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-385308-105":59,"doc-detail-385308-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","a-highly-sensitive-pan-cancer-test-for-microsatellite-instability","A Highly Sensitive Pan-Cancer Test for Microsatellite Instability","","A multiplex long mononucleotide repeat (LMR) assay was developed to enable highly sensitive microsatellite instability (MSI) detection across multiple cancer types. The assay tested 469 tumor samples from 20 cancers, including 319 from Lynch syndrome patients. LMR results were validated against deficient mismatch repair (dMMR) status using immunohistochemistry as reference and compared with the Promega pentaplex MSI panel. Sensitivity for dMMR detection was 99% for CRC and 96% for non-CRC, with overall agreement of 99% for CRC and 89% for non-CRC. 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Overall percent agreement between the LMR and Promega pentaplex panels was 99% for CRC and 89% for non-CRC tumors.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},385308,1790318377,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":29,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},2336478940917,"https://ap-avatar.wpscdn.com/davatar_6f874abed73319feea01a86fa6f0fab8","The Journal of Molecular Diagnostics, Vol. 25, No. 11, November 2023  \nA Highly Sensitive Pan-Cancer Test for Microsatellite Instability  \nJeffery W. Bacher, *y Eshwar B. Udho, * Ethan E. Strauss,z Irina Vyazunova, * Steven Gallinger,x Daniel D. Buchanan,{k ** Rish K. Pai,yy Allyson S. Templeton,zz Douglas R. Storts, * James R. Eshleman,xx and Richard B. Halbergy{{kk  \nFrom R&D Clinical Diagnostics* and R&D Bioinformatics,z Promega Corporation, Madison, Wisconsin; the Department of Mediciney and Department of Oncology,{{ McArdle Laboratory of Cancer Research, University of Wisconsin, Madison, Wisconsin; the Mount Sinai Hospital,x University of Toronto, Toronto, Ontario, Canada; the Colorectal Oncogenomics Group,{ Department of Clinical Pathology, The University of Melbourne, Parkville, Victoria, Australia; the University of Melbourne Centre for Cancer Research, k Victorian Comprehensive Cancer Centre, Parkville, Victoria, Australia; the Genomic Medicine and Family Cancer Clinic,** Royal Melbourne Hospital, Parkville, Victoria, Australia; Health Science Research,yy Mayo Clinic, Scottsdale, Arizona; the Fred Hutchinson Cancer Center,zz Seattle, Washington; the School of Medicine,xx Johns Hopkins University, Baltimore, Maryland; and the University of Wisconsin Carbone Cancer Center,kk Madison, Wisconsin  \nAccepted for publication July 18, 2023 .  \nAddress correspondence to Jeffery W. Bacher, Ph.D., RD Clinical Diagnostics Group, Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711-5399; or Richard B. Halberg, Ph.D., Departments of Medicine and Oncology, University of Wisconsin, Madison, Wisconsin, 7533 WIMR II, 600 Highland Ave., Madison, WI 53792.  \nE-mail: jeff.bacher@promega. com or richard.halberg@ [medicine.wisc.edu](medicine.wisc.edu).  \nMicrosatellite instability (MSI) is an evolving biomarker for cancer detection and treatment. MSI was first used to identify patients with Lynch syndrome, a hereditary form of colorectal cancer (CRC), but has recently become indispensable in predicting patient response to immunotherapy. To address the need for pan-cancer MSI detection, a new multiplex assay was developed that uses novel long mononucleotide repeat (LMR) markers to improve sensitivity. A total of 469 tumor samples from 20 different cancer types, including 319 from patients with Lynch syndrome, were tested for MSI using the new LMR MSI Analysis System. Results were validated by using deficient mismatch repair (dMMR) status according to immunohistochemistry as the reference standard and compared versus the Promega pentaplex MSI panel. The sensitivity of the LMR panel for detection of dMMR status by immunohistochemistry was 99% for CRC and 96% for non-CRC. The overall percent agreement between the LMR and Promega pentaplex panels was 99% for CRC and 89% for non-CRC tumors. An increased number of unstable markers and the larger size shifts observed in dMMR tumors using the LMR panel increased confidence in MSI determinations. The LMR MSI Analysis System expands the spectrum of cancer types in which MSI can be accurately detected. (J Mol Diagn 2023, 25: 806e826; [https://doi.org/10.1016/j.jmoldx.2023.07.003](https://doi.org/10.1016/j.jmoldx.2023.07.003))  \nMicrosatellite Instability  \nMicrosatellites are 1 to 6 bp short tandem DNA repeats constituting approximately 3% of the human genome.1 Microsatellites are prone to DNA replication errors resulting from polymerase slippage, which are effectively  \ncorrected by the DNA mismatch repair (MMR) system.2 Inactivation of any of the MMR genes (MLH1, MSH2, MSH6, and PMS2) results in hypermutability of these microsatellite repeats, a condition referred to as microsatellite instability (MSI) .3e6 Individuals carrying a germline pathogenic variant in one copy of an MMR gene are said to have  \nThe Colon Cancer Family Registry (CCFR) is supported in part by funding from the National Cancer Institute, NIH (award U01 CA167551) . Support for case ascertainment was provided in part from the","cbCaipD5W91l7hX8","https://ap.wps.com/l/cbCaipD5W91l7hX8","pdf",1931666,21,"English","# A Highly Sensitive Pan-Cancer Test for Microsatellite Instability\n## Microsatellite Instability\n## MSI-High/Deficient Mismatch Repair Detection","[{\"question\":\"What problem does the study address?\",\"answer\":\"The study addresses the need for sensitive pan-cancer detection of microsatellite instability (MSI), including accurate identification of deficient mismatch repair (dMMR) status.\"},{\"question\":\"How was the new MSI test validated?\",\"answer\":\"Validation used dMMR status determined by immunohistochemistry as the reference standard, and results were compared with the Promega pentaplex MSI panel.\"},{\"question\":\"What were the reported performance results for CRC and non-CRC?\",\"answer\":\"For dMMR detection by immunohistochemistry, the sensitivity was 99% for CRC and 96% for non-CRC. Overall percent agreement between the LMR and Promega pentaplex panels was 99% for CRC and 89% for non-CRC tumors.\"}]","A Highly Sensitive Pan-Cancer Test for Microsatellite Instability | PDF",1790267942,53]