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It analyzes genetic and DNA methylation alterations, survival outcomes, co-expression patterns, and immune infiltration using multiple computational tools. Functional EdU and transwell assays test effects on proliferation, migration, and invasion, while xenograft and transplantation models assess immunotherapy response in TNBC with PD-1/PD-L1 inhibition. P4HA3 expression is associated with tumor microenvironment infiltration and EMT-related markers, while P4HA3 deficiency suppresses malignant behavior and enhances anti-tumor immunotherapy.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & Report",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/a-computational-analysis-of-the-oncogenic-and-anti-tumor-immunity-role-of-p4ha3-in-human-cancers/332761/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/a-computational-analysis-of-the-oncogenic-and-anti-tumor-immunity-role-of-p4ha3-in-human-cancers/332761.png","ImageObject",300,407,{"name":42,"@type":43},"Quinn","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-11-04","2026-09-21",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",6,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What is P4HA3 and why is it studied in cancers?","Question",{"text":63,"@type":64},"P4HA3 is a procollagen synthesis protein. Its role in cancer development and anti-tumor immunity is not well known, motivating a pan-cancer investigation of its expression, prognostic value, and immune associations.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How was P4HA3 expression and genomic context evaluated across cancers?",{"text":68,"@type":64},"P4HA3 expression was analyzed using TIMER2.0, GTEx, GEPIA2.0, and TCGA. The study further examined genetic and DNA methylation alterations, survival, and protein co-expression across additional portals and datasets.",{"name":70,"@type":61,"acceptedAnswer":71},"What functional evidence supports P4HA3’s role in tumor progression and immunotherapy?",{"text":72,"@type":64},"EdU and transwell assays assessed effects on proliferation, migration, and invasion, showing that P4HA3 deficiency inhibits these tumor cell capabilities. Xenograft and subcutaneous transplantation models in TNBC were used to link P4HA3 with response to PD-1/PD-L1 immunotherapy.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},332761,1790183597,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,105,110,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":102,"show_sort_weight":103,"slug":104},"Technology",50,"technology",{"id":106,"doc_module":4,"doc_module_name":25,"category_name":107,"show_sort_weight":108,"slug":109},7,"Healthcare",40,"healthcare",{"id":111,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":112,"slug":113},8,30,"research-report",{"id":115,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},9,"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":97,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":111,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":127,"language":139,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":12,"update_tm":143,"read_time":144,"preview_page_count":4},962075114765,"https://ap-avatar.wpscdn.com/davatar_a8503ba1806abce46bf441b54a3ca4cd","PLOS COMPUTATIONAL BIOLOGY  \nOPEN ACCESS  \nCitation: Huang HY, Zhang FW, Yu J, Xiao YH, Zhu D, Yi X, et al. (2024) A computational analysis of the oncogenic and anti-tumor immunity role of P4HA3 in human cancers. PLoS Comput Biol 20(11): e1012284 . [https://doi.org/10.1371/journal](https://doi.org/10.1371/journal). pcbi.1012284  \nEditor: Hailin Tang, Sun Yat-Sen University Cancer Center, CHINA  \nReceived: July 4, 2024  \nAccepted: October 9, 2024  \nPublished: November 6, 2024  \nPeer Review History: PLOS recognizes the benefits of transparency in the peer review process; therefore, we enable the publication of all of the content of peer review and author responses alongside final, published articles. The editorial history of this article is available here:  \n[https://doi.org/10.1371/journal.pcbi.1012284](https://doi.org/10.1371/journal.pcbi.1012284)  \n[Copyright:](Copyright:) © [2024 Huang](2024 Huang) et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.  \nData Availability Statement: All relevant data are in the manuscript and its Supporting information files.  \nRESEARCH ARTICLE  \nA computational analysis of the oncogenicand anti-tumor immunity role of P4HA3 inhuman cancers  \nHong Yan Huang1☯, Fu Wei Zhang2☯, Jie Yu3☯, Yan Hong Xiao4,5, Di Zhu1, XiaoLin Yi1, XiaoHua Lin1, Ming Jin6, Hai Yun Jin7 *, Yong Sheng Huang8 *, Shu Wei Ren4,5 *  \n1 Department of Breast Surgery, Zhujiang Hospital, Southern Medical University, Guangdong, China,  \n2 Department of Thoracic Surgery, Zhujiang Hospital, Southern Medical University, Guangdong, China, 3 Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangdong, China, 4 Department of Clinical Laboratory, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangdong, China, 5 Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangdong, China, 6 Department of Gastroenterology, Shenzhen Hospital, Southern Medical University, Guangdong, China, 7 Department of Gynaecology and Obstetrics, Southern Hospital Taihe Branch, Southern Medical University, Guangdong, China, 8 Cellular & Molecular Diagnostics Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangdong, China  \n☯ These authors contributed equally to this work.  \n* [shucai42521@126.com](shucai42521@126.com) (HYJ); [huangysh65@mail.sysu.edu.cn](huangysh65@mail.sysu.edu.cn) (YSH); [renshw3@mail.sysu.edu.cn](renshw3@mail.sysu.edu.cn) (SWR)  \nAbstract  \nProlyl-4-hydroxylase subunit alpha3 (P4HA3) is a triple helical procollagen synthesis protein. The role of P4HA3 in cancer development is not well known and lacks comprehensive analyses among human cancers. This study aimed to investigate the relationship between P4HA3 expression and anti-tumor immunity and its prognostic value in pan-cancer. P4HA3 expression was analyzed from TIMER2 .0, GTEx, GEPIA2 .0 and TCGA databases. Genetic and DNA methylation alterations, survival analysis and proteins co-expression analysis of P4HA3 in cBio Cancer Genomics Portal, TCGA, GSCA and TIMER2 .0. The correlation between P4HA3 expression and immune infiltration was analyzed by TIDE, XCELL, MCPCOUNTER, and EPIC. We performed EdU and transwell experiments to evaluate the influence of P4HA3 on the proliferation , migration and invasion abilities of different tumors. Patients derived xenograft (PDX) and subcutaneous transplantation models were utilized to explore the correlation between P4HA3 and immunotherapy response in triple-negative breast cancer (TNBC) . Among 33 types of cancers, P4HA3 had generally different expression between different tumors, further analysis showed that the expression of P4HA3 was correlated with the cells infiltration of the tumor microenvironment (TME) . The expression of P4HA3 was positively with the cell proliferation mar","cbCaiiAg2pAITYCR","https://ap.wps.com/l/cbCaiiAg2pAITYCR","pdf",5495272,"English","# Abstract\n## Expression and data integration\n## Immune infiltration, prognosis, and alterations\n## Functional validation and immunotherapy response\n## Conclusions and biomarker potential","[{\"question\":\"What is P4HA3 and why is it studied in cancers?\",\"answer\":\"P4HA3 is a procollagen synthesis protein. Its role in cancer development and anti-tumor immunity is not well known, motivating a pan-cancer investigation of its expression, prognostic value, and immune associations.\"},{\"question\":\"How was P4HA3 expression and genomic context evaluated across cancers?\",\"answer\":\"P4HA3 expression was analyzed using TIMER2.0, GTEx, GEPIA2.0, and TCGA. The study further examined genetic and DNA methylation alterations, survival, and protein co-expression across additional portals and datasets.\"},{\"question\":\"What functional evidence supports P4HA3’s role in tumor progression and immunotherapy?\",\"answer\":\"EdU and transwell assays assessed effects on proliferation, migration, and invasion, showing that P4HA3 deficiency inhibits these tumor cell capabilities. Xenograft and subcutaneous transplantation models in TNBC were used to link P4HA3 with response to PD-1/PD-L1 immunotherapy.\"}]","A computational analysis of the oncogenic and anti-tumor immunity role of P4HA3 in human cancers | PDF",1789999419,48]