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This study evaluates PFKP expression across multiple cancer types (pan-cancer), links it to patient prognosis, and tests its functional impact in lung cancer cells. Using TCGA and GEO analyses plus in vitro assays, results show PFKP as a prognostic risk factor and a driver of tumor cell growth and migration.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/a-comprehensive-prognostic-and-immunological-implications-of-pfkp-in-pan-cancer/328851/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/a-comprehensive-prognostic-and-immunological-implications-of-pfkp-in-pan-cancer/328851.png","ImageObject",300,407,{"name":92,"@type":93},"Levi","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-04","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":24},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the main research goal regarding PFKP in pan-cancer?","Question",{"text":112,"@type":113},"To assess PFKP expression and its prognostic and immunological implications across multiple tumor types, and to determine its potential value as a therapeutic target.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was PFKP analyzed in this study?",{"text":117,"@type":113},"The study assessed PFKP expression, immune cell infiltration, and patient prognosis using TCGA and GEO, then performed multiple lung cancer cell experiments including Western blot, proliferation and migration assays, and flow cytometry.",{"name":119,"@type":110,"acceptedAnswer":120},"What functional effects does PFKP have on lung cancer cells?",{"text":121,"@type":113},"PFKP overexpression promotes proliferation and migration while inhibiting apoptosis; PFKP deficiency produces opposite effects.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},328851,1791448773,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":24,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},7971461740909,"https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d","Ling et al. Cancer Cell International (2024) 24:310 Cancer Cell International  \n[https://doi.org/10.1186/s12935-024-03497-w](https://doi.org/10.1186/s12935-024-03497-w)  \nRESEARCH Open Access  \nA comprehensive prognostic and immunological implications of PFKPin pan-cancer  \nXiaodong Ling 1, Luquan Zhang 1, Chengyuan Fang 1, Hao Liang 1 and Jianqun Ma 1*  \nAbstract  \nBackground Phosphofructokinase P (PFKP) is a key rate-limiting enzyme in glycolysis, playing a crucial role in various pathophysiological processes. However, its specific function in tumors remains unclear. This study aims to evaluate the expression and specific role of PFKP across multiple tumor types (Pan-cancer) and to explore its potential clinical significance as a therapeutic target in cancer treatment.  \nMethods We analyzed the expression of PFKP, immune cell infiltration, and patient prognosis across various cancers using data from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Additionally, we conducted a series of experiments in lung cancer cells, including Western blot, CCK-8 assay, colony formation assay, transwell migration assay, scratch wound healing assay, LDH release assay, and flow cytometry, to evaluate the impact of PFKP on tumor cells.  \nResults PFKP was found to be highly expressed in most cancers and identified as a prognostic risk factor. Elevated PFKP expression is associated with poorer clinical outcomes, particularly in lung adenocarcinoma (LUAD) . Receiver operating characteristic (ROC) curve analysis indicated that PFKP can effectively differentiate between cancerous and normal tissues. The expression of PFKP in most tumors showed significant correlations with tumor mutational burden (TMB), microsatellite instability (MSI), immune score, and immune cell infiltration. In vitro experiments demonstrated that PFKP overexpression promotes lung cancer cell proliferation and migration while inhibiting apoptosis, whereas PFKP deficiency results in the opposite effects.  \nConclusion PFKP acts as an oncogene involved in tumorigenesis and may influence the immune microenvironment within the tumor. Our findings suggest that PFKP could serve as a potential biomarker for predicting prognosis and the efficacy of immunotherapy in tumors.  \nKeywords PFKP, Pan-cancer, LUAD, Prognosis prediction, Immune analysis  \n*Correspondence:  \nJianqun Ma  \n[jianqunma@hrbmu.edu.cn](jianqunma@hrbmu.edu.cn)  \n1Department of Thoracic Surgery, Harbin Medical University Cancer Hospital, 150 Haping Road, Harbin, Heilongjiang 150040, China  \n© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit [http://](http://)[ ](http://)[creativecommons.org/l](creativecommons.org/l)icenses/by-nc-nd/4.0/.  \nLing et al. Cancer Cell International (2024) 24:310  \nIntroduction  \nCancer remains one of the leading causes of death worldwide, with high incidence and mortality rates, making ita critical public health concern. Traditional cancer treatments, such as surgery, radiation therapy, and chemotherapy, have been supplemented in recent year","cbCaim6o9UP0uySy","https://ap.wps.com/l/cbCaim6o9UP0uySy","pdf",4045438,15,"English","# Abstract\n## Background\n## Methods\n## Results\n## Conclusion\n# Introduction","[{\"question\":\"What is the main research goal regarding PFKP in pan-cancer?\",\"answer\":\"To assess PFKP expression and its prognostic and immunological implications across multiple tumor types, and to determine its potential value as a therapeutic target.\"},{\"question\":\"How was PFKP analyzed in this study?\",\"answer\":\"The study assessed PFKP expression, immune cell infiltration, and patient prognosis using TCGA and GEO, then performed multiple lung cancer cell experiments including Western blot, proliferation and migration assays, and flow cytometry.\"},{\"question\":\"What functional effects does PFKP have on lung cancer cells?\",\"answer\":\"PFKP overexpression promotes proliferation and migration while inhibiting apoptosis; PFKP deficiency produces opposite effects.\"}]","A comprehensive prognostic and immunological implications of PFKP in pan-cancer | PDF",1789981353,38]