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Because GBM relies on aerobic glycolysis, glucose metabolism offers a potential vulnerability. This study evaluates PFKFB4, a regulatory kinase in glucose metabolism, and tests 5MPN, a PFKFB4 inhibitor, for effects on glycolysis and invasiveness in GBM cells. Bioinformatic analyses use TCGA and CGGA datasets to link PFKFB4 expression with prognosis and gene interactions, then experimental results assess metabolic and invasive outcomes.",{"@graph":69,"@context":126},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/5mpn-effectively-targets-pfkfb4-and-inhibits-the-glucose-metabolism-process-and-invasion-of-glioblastoma-research-paper-summary/448435/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/5mpn-effectively-targets-pfkfb4-and-inhibits-the-glucose-metabolism-process-and-invasion-of-glioblastoma-research-paper-summary/448435.png","ImageObject",300,407,{"name":92,"@type":93},"Mia  ","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-05","2026-09-30",true,{"@type":102,"interactionType":103,"userInteractionCount":52},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118,122],{"name":109,"@type":110,"acceptedAnswer":111},"What is the main therapeutic target investigated in this study?","Question",{"text":112,"@type":113},"The study focuses on PFKFB4, a key regulatory kinase involved in glucose metabolism, and evaluates its relevance to glioblastoma progression.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was 5MPN assessed in glioblastoma cells?",{"text":117,"@type":113},"5MPN was tested for its ability to inhibit glycolysis and invasiveness in GBM cell lines, with reported IC50 values indicating dose-dependent suppression.",{"name":119,"@type":110,"acceptedAnswer":120},"What do the results show about PFKFB4 expression and prognosis?",{"text":121,"@type":113},"PFKFB4 is highly expressed in glioblastoma multiforme and correlates with poor prognosis according to analyses of TCGA and CGGA datasets.",{"name":123,"@type":110,"acceptedAnswer":124},"What is the key implication of the study’s findings?",{"text":125,"@type":113},"Targeting PFKFB4 using 5MPN shows significant anti-glycolytic and anti-invasive effects, supporting PFKFB4 as a potential therapeutic strategy and suggesting further mechanistic and clinical evaluation.","https://schema.org",{"og:url":83,"og:type":128,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":130,"canonical":83},"index,follow",{"doc_id":132,"site_id":62},448435,1790796280,{"code":4,"msg":5,"data":135},{"doc_id":132,"user_id":136,"nickname":92,"user_avatar":137,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":138,"file_id":139,"file_url":140,"file_type":141,"file_size":142,"view_count":52,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":143,"language":144,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":145,"faqs":146,"seo_title":147,"seo_description":67,"update_tm":148,"read_time":149,"preview_page_count":4},687207024478,"https://ap-avatar.wpscdn.com/davatar_a8503ba1806abce46bf441b54a3ca4cd","5MPN effectively targets PFKFB4 and inhibits the glucose metabolism process and invasion of glioblastoma  \nChen Yu1, Zhennan Tao1,2,3, Tianyu Lu1,2,3, Hongbin Ni1,2, Weibang Liang1,2,3  \n1Department of Neurosurgery, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing, China; 2Department of Neurosurgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, China; 3Neurosurgical Institute, Nanjing University, Nanjing, China  \nContributions: (I) Conception and design: C Yu, W Liang; (II) Administrative support: W Liang; (III) Provision of study materials or patients: T Lu; (IV) Collection and assembly of data: H Ni; (V) Data analysis and interpretation: Z Tao, T Lu; (VI) Manuscript writing: All authors; (VII) Final approval  \nof manuscript: All authors.  \nCorrespondence to: Weibang Liang, MD, PhD. Department of Neurosurgery, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, No. 321 Zhongshan Road, Nanjing 210008, China; Department of Neurosurgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, China; Neurosurgical Institute, Nanjing University, Nanjing, [China. Email: neurosurgery12c@163.com](China. Email: neurosurgery12c@163.com).  \nBackground: Gliomas are the most common malignant tumors of the central nervous system, with glioblastoma multiforme (GBM) being the most aggressive and having the poorest prognosis. Although there are few targeted therapies available, the glucose metabolism of GBM, characterized by aerobic glycolysis, presents a potential therapeutic target. While many studies have focused on glucose metabolism in GBM, research on phosphofructokinase-2/fructose-2,6-bisphosphatase 4 (PFKFB4), a key regulatory kinase, remains limited. This study aims to investigate the inhibitory effects of targeting PFKFB4 in GBM and  \nexplore its molecular mechanisms.  \nMethods: In this study, we analyzed the expression levels of PFKFB4, its correlation with prognosis, and its relationship with other genes using The Cancer Genome Atlas (TCGA) and Chinese Glioma Genome Atlas (CGGA) datasets. We also selected 5MPN (a specific PFKFB4 inhibitor not yet studied in GBM) as the focus of our experiments, evaluating its impact on glucose metabolism and invasiveness  \nin GBM cells.  \nResults: Our study found that PFKFB4 is highly expressed in GBM and correlates with poor prognosis.  \n5MPN [half-maximal inhibitory concentration (IC50) of 14.62 μM in U251 cells and 10.15 μM in T98 cells] effectively inhibits glycolysis (at 5 μM) and invasiveness (at 2 μM) in GBM cells. We also explored potential molecular mechanisms that may work synergistically with PFKFB4, supported by data from  \npublic databases.  \nConclusions: PFKFB4 shows therapeutic potential as a target for GBM treatment, and its inhibitor 5MPN significantly inhibits glycolysis and invasiveness in GBM. This study provides new insights and  \npotential drug options for targeted therapy in GBM.  \nKeywords: Glioblastoma; phosphofructokinase-2/fructose-2,6-bisphosphatase 4 (PFKFB4); 5MPN; glucose  \nmetabolism; invasion  \nSubmitted Aug 07, 2025. Accepted for publication Oct 14, 2025. Published online Dec 23, 2025.  \ndoi: 10.21037/tcr-2025-1740  \nView this article at: [https://dx.doi.org/10.21037/tcr-2025-1740](https://dx.doi.org/10.21037/tcr-2025-1740)  \n© AME Publishing Company. Transl Cancer Res 2025;14(12):8243-8253 | [https://dx.doi.org/10.21037/tcr-2025-1740](https://dx.doi.org/10.21037/tcr-2025-1740)  \n8244 Yu et al. 5MPN inhibits glioblastoma by targeting PFKFB4  \nIntroduction  \nGlioma is the most common malignant tumor in the central nervous system, with glioblastoma multiforme (GBM) defined as World Health Organization (WHO) grade 4, having a very poor prognosis and being prone to recurrence (1) . Currently, except for temozolomide, no effective chemotherapy has significantly benefited GBM patients. Adjuvant chemotherapy drugs such ","cbCaiffaYLah75Gu","https://ap.wps.com/l/cbCaiffaYLah75Gu","pdf",7550751,11,"English","# Background\n## Glioblastoma and targeted therapy need\n## Aerobic glycolysis and Warburg effect\n## PFKFB4 as a regulatory kinase\n# Methods\n## Bioinformatic analysis using TCGA and CGGA\n## In vitro evaluation of 5MPN in GBM cells\n# Results\n## PFKFB4 expression and prognostic relevance\n## 5MPN inhibition of glycolysis and invasiveness\n## Proposed molecular mechanisms\n# Conclusions\n## Therapeutic potential of targeting PFKFB4 with 5MPN","[{\"question\":\"What is the main therapeutic target investigated in this study?\",\"answer\":\"The study focuses on PFKFB4, a key regulatory kinase involved in glucose metabolism, and evaluates its relevance to glioblastoma progression.\"},{\"question\":\"How was 5MPN assessed in glioblastoma cells?\",\"answer\":\"5MPN was tested for its ability to inhibit glycolysis and invasiveness in GBM cell lines, with reported IC50 values indicating dose-dependent suppression.\"},{\"question\":\"What do the results show about PFKFB4 expression and prognosis?\",\"answer\":\"PFKFB4 is highly expressed in glioblastoma multiforme and correlates with poor prognosis according to analyses of TCGA and CGGA datasets.\"},{\"question\":\"What is the key implication of the study’s findings?\",\"answer\":\"Targeting PFKFB4 using 5MPN shows significant anti-glycolytic and anti-invasive effects, supporting PFKFB4 as a potential therapeutic strategy and suggesting further mechanistic and clinical evaluation.\"}]","5MPN effectively targets PFKFB4 and inhibits the glucose metabolism process and invasion of glioblastoma - Research paper summary | PDF",1790727010,28]