[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-420359-105":59,"doc-detail-420359-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","5-azacytidine-enhances-differentiation-of-human-placenta-derived-mesenchymal-stem-cells-towards-schwann-like-cells","5-Azacytidine Enhances Differentiation of Human Placenta-derived Mesenchymal Stem Cells Towards Schwann-like Cells","","Mesenchymal stem cells support regenerative medicine, especially for neurodegenerative diseases and peripheral nerve injuries. Human placenta-derived mesenchymal stem cells (hPMSCs) were isolated, cultured, and characterized as MSCs by CD73, CD90, and CD105 expression. Multilineage differentiation confirmed their multipotency. hPMSCs were then induced into Schwann cell-like cells in Schwann differentiation medium with or without 5-azacytidine. After 14 days, 5-aza increased Schwann marker gene expression (S100β, P75, GFAP, PMP22) and elevated GFAP and MBP proteins, indicating enhanced differentiation.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/5-azacytidine-enhances-differentiation-of-human-placenta-derived-mesenchymal-stem-cells-towards-schwann-like-cells/420359/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/5-azacytidine-enhances-differentiation-of-human-placenta-derived-mesenchymal-stem-cells-towards-schwann-like-cells/420359.png","ImageObject",300,407,{"name":92,"@type":93},"OmBimo","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-09-29","2026-09-28",true,{"@type":102,"interactionType":103,"userInteractionCount":8},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What was the main objective of this study?","Question",{"text":112,"@type":113},"To isolate and induce differentiation of human placenta-derived mesenchymal stem cells (hPMSCs) into Schwann cell-like cells (SC-like cells) that support peripheral nervous system function.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were hPMSCs characterized before differentiation induction?",{"text":117,"@type":113},"Isolated hPMSCs were confirmed as MSCs by surface marker positivity for CD73, CD90, and CD105, and their multipotency was validated by differentiation into osteocytes, chondrocytes, and adipocytes.",{"name":119,"@type":110,"acceptedAnswer":120},"What effect did 5-azacytidine have on Schwann cell-like differentiation?",{"text":121,"@type":113},"Adding 5-azacytidine (5-aza) significantly enhanced differentiation, producing higher expression of Schwann-specific genes (S100β, P75, GFAP, PMP22) and increased GFAP and MBP protein levels compared with control conditions.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},420359,1790642901,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":8,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},962090893581,"https://ap-avatar.wpscdn.com/davatar_276721f389ce27ea32af1340a28f341c","doi: 10.21873/invivo.14187  \n5-Azacytidine Enhances Differentiation of Human Placenta-derived Mesenchymal Stem Cells Towards Schwann-like Cells  \nAREECHUNSOTTHIBUNDHU1, WILASINEE PROMJANTUEK2, PHONGSAKORN KUNHORM2, SITAKAN NATPHOPSUK1, NIPHA CHAICHAROENAUDOMRUNG2 and PARINYA NOISA2  \n1Chulabhorn International College of Medicine, Thammasat University, Patumthani, Thailand;  \n2 Laboratory of Cell-Based Assays and Innovations, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand  \nAbstract  \nBackground/Aim: Mesenchymal stem cells (MSCs) can be used for regenerative medicine, particularly in the treatment of neurodegenerative diseases and peripheral nerve injuries. Among these, human placenta-derived mesenchymal stem cells (hPMSCs) possess a high potential for differentiation into various cell types, including those of the neural lineage under appropriate conditions. The primary aim of this study was to isolate and induce the differentiation of hPMSCs into Schwann cell-like cells (SC-like cells) that function to support and play a role in the peripheral nervous system. Materials and Methods: Human placental tissues were isolated, and hPMSCs were cultured and characterized. The isolated hPMSCs were positive formesenchymal stem cell surface markers CD73, CD90, and CD105. The multipotency of hPMSCs was confirmed by their ability to differentiate into osteocytes, chondrocytes, and adipocytes. To induce Schwann cell differentiation, hPMSCs were cultured in Schwann cell differentiation medium with or without the addition of 5-azacytidine (5-aza). Gene and protein expression analyses were performed to assess Schwann cell-specific markers. Results: After 14 days of induction, hPMSCs differentiated in Schwann cell differentiation medium showed significant upregulation of Schwann cell-specific genes S100β, P75, GFAP, and PMP22 (p\u003C0.01). Interestingly, the group treated with 5-aza exhibited even higher expression levels of these genes compared to the Schwann cell differentiation medium alone and the control group (p\u003C0.01) . Furthermore, protein expression analysis demonstrated that glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP) were highly expressed in the 5-aza-treated condition, confirming enhanced Schwann cell-like differentiation.  \nConclusion: This study demonstrates that hPMSCs can be successfully differentiated into Schwann cell-like cells. The addition of 5-aza significantly promoted this differentiation, leading to higher expression of Schwann cell-specific  \ncontinued  \n✉ Parinya Noisa, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon  \nRatchasima 30000, Thailand. Tel: +66 616266390, Fax: +66 44224154, [e-mail:](e-mail: p.noisa@sut.ac.th)[ p.noisa@sut.ac.th](e-mail: p.noisa@sut.ac.th)  \nReceived September 18, 2025 | Revised October 24, 2025 | Accepted November 20, 2025  \nThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.  \n©2026 The Author(s) . Anticancer Research is published by the International Institute of Anticancer Research.  \ngenes and proteins. These findings suggest that 5-aza plays a supportive role in enhancing Schwann cell differentiation from hPMSCsand may provide a promising approach for future clinical applications in peripheral nerve regeneration.  \nKeywords: Human placenta, mesenchymal stem cells, Schwann cells, 5-azacytidine, DNA demethylation.  \nIntroduction  \nMesenchymal stem cells (MSCs) possess the capability to differentiate into various cell types, including neurons and glial cells within the nervous system (1) . Consequently, understanding the mechanisms that drive MSC differentiation into specific neuronal lineages is crucial for developing cell-based therapies for neurological disorders. This is particula","cbCaie4AP8ShU5LL","https://ap.wps.com/l/cbCaie4AP8ShU5LL","pdf",3735450,14,"English","# Abstract\n# Background/Aim\n# Materials and Methods\n# Results\n# Conclusion\n# Keywords\n# Introduction","[{\"question\":\"What was the main objective of this study?\",\"answer\":\"To isolate and induce differentiation of human placenta-derived mesenchymal stem cells (hPMSCs) into Schwann cell-like cells (SC-like cells) that support peripheral nervous system function.\"},{\"question\":\"How were hPMSCs characterized before differentiation induction?\",\"answer\":\"Isolated hPMSCs were confirmed as MSCs by surface marker positivity for CD73, CD90, and CD105, and their multipotency was validated by differentiation into osteocytes, chondrocytes, and adipocytes.\"},{\"question\":\"What effect did 5-azacytidine have on Schwann cell-like differentiation?\",\"answer\":\"Adding 5-azacytidine (5-aza) significantly enhanced differentiation, producing higher expression of Schwann-specific genes (S100β, P75, GFAP, PMP22) and increased GFAP and MBP protein levels compared with control conditions.\"}]","5-Azacytidine Enhances Differentiation of Human Placenta-derived Mesenchymal Stem Cells Towards Schwann-like Cells | PDF",1790620637,35]